LOCUS BC011609 3041 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens DnaJ (Hsp40) homolog, subfamily B, member 2, mRNA
(cDNA clone MGC:3506 IMAGE:3619734), complete cds.
ACCESSION BC011609
VERSION BC011609.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3041)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3041)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC011609.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Louis M. Staudt, M.D., Ph.D.
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 12 Row: h Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34222304
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..3041
/db_xref="H-InvDB:HIT000035327"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3506 IMAGE:3619734"
/tissue_type="Lymph, Burkitt lymphoma"
/clone_lib="NIH_MGC_8"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3041
/gene="DNAJB2"
/gene_synonym="HSPF3"
/db_xref="GeneID:3300"
/db_xref="HGNC:HGNC:5228"
/db_xref="MIM:604139"
CDS 180..1154
/gene="DNAJB2"
/gene_synonym="HSPF3"
/codon_start=1
/product="DNAJB2 protein"
/protein_id="AAH11609.1"
/db_xref="GeneID:3300"
/db_xref="HGNC:HGNC:5228"
/db_xref="MIM:604139"
/translation="MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEK
KFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEV
FREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPG
AGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLEL
SRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGG
REAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL"
BASE COUNT 587 a 872 c 968 g 614 t
ORIGIN
1 gcaccgctga cgcatgcctg cgcgcacagc tgggccgggc gcgtcctacg cagcagccgc
61 gagccgggct gcgggtgcca gacggttccc ggcggggggc aggggcgggg cgccgcagga
121 ggccgggact cctggcggag gagccccaag gaggcccgcc tgacgactga ccagttgcca
181 tggcatccta ctacgagatc ctagacgtgc cgcgaagtgc gtccgctgat gacatcaaga
241 aggcgtatcg gcgcaaggct ctccagtggc acccagacaa aaacccagat aataaagagt
301 ttgctgagaa gaaatttaag gaggtggccg aggcatatga agtgctgtct gacaagcaca
361 agcgggagat ttacgaccgc tatggccggg aagggctgac agggacagga actggcccat
421 ctcgggcaga agctggcagt ggtgggcctg gcttcacctt caccttccgc agccccgagg
481 aggtcttccg ggaattcttt gggagtggag acccttttgc agagctcttt gatgacctgg
541 gccccttctc agagcttcag aaccggggtt cccgacactc aggccccttc tttaccttct
601 cttcctcctt ccctgggcac tccgatttct cctcctcatc tttctccttc agtcctgggg
661 ctggtgcttt tcgctctgtt tctacatcta ccacctttgt ccaaggacgc cgcatcacca
721 cacgcagaat catggagaac gggcaggagc gggtggaagt ggaggaggat gggcagctga
781 agtcagtcac aatcaatggt gtcccagatg acctggcact gggcttggag ctgagccgtc
841 gcgagcagca gccgtcagtc acttccaggt ctgggggcac tcaggtccag cagacccctg
901 cctcatgccc cttggacagc gacctctctg aggatgagga cctgcagctg gccatggcct
961 acagcctgtc agagatggag gcagctggga agaaacccgc aggtgggcgg gaggcacagc
1021 accgacggca ggggcggccc aaggcccagc accaagatcc aggcttgggg gggacccagg
1081 agggtgcgag gggtgaagca accaaacgca gtccatcccc agaggagaag gcctctcgct
1141 gcctcatcct ctgaacaccg ggcccaacct gatctgatcc agatcttgac tggggggtct
1201 gactcactgt gggaagagaa gaggggagta tcctgagttg taggaactgc tttccaactc
1261 caagctccct ccacaagttt ccctcccagg ccccccacac cccagtgtgg acttgggatt
1321 tgctgtgctc agcccagggc tgataggtcc ctggtgaagc ccagggtggg gggtgtcagg
1381 gcagtggagg ggcccgagga gccaggttgc atttattgga tggggagctc caaggggcat
1441 tagtggtttg ggctgggcct tttgtgccct ggtactctgc cacctgtgtt gctgatggtg
1501 tcaaggaagg aggacttggc ctagggttgt ctgagccgga gccggcagct ccactggaga
1561 gcagtgcagg cagagtggag cctcctgctc tcctggacca gctgcagacc cccaaccctg
1621 gtttctgtgc catgttgcgc tctgaccgtc tctgttgctt ctcttctggt gttgcttctc
1681 ctccctccca ttctctctgc aactccctgc gggccgcatc gcttgctttc actgccgtct
1741 ggctaggact cccttcttcc ttccttcccc gagaaggcct caatgtggcg aggaagatgc
1801 tggggccggt agggctgtga gatcttctgg ggaggctagc cgggtggggc gggagcctct
1861 cagctgtcca gattcagaac tggagcccac tcctcctccc tctcgttgcc tcagcctgcc
1921 ctcaccctca ggactaggca gaggtgaggc tggctcaccc tgaagaggtg ggataggacc
1981 gggggacccc agagggaggc ctaggagggg actgcaccca tactgcttcc ctaccacaaa
2041 tcagggctca gggagaggcc atgcggccag cccaggtctg catgctgagc cccatcctcc
2101 acagcttgcc gctgacgctc tctcctgtca ccccgcccct gctctctccc cagatgtgtt
2161 ctgagctgga tgccgggttc cagaatcgct gcacagttcc aacaggacag cgccttcccc
2221 catgcgctgg gaggggaccc tccatttctc cccctcaccc atgctgagtg tagagccggg
2281 gcctgggtgg cgggtggggg ccgggtggga ggtggcagta gtcttagcct gtgcactctc
2341 ttccttgggt gtttggtgct ggctcctggg gactacaaat cccagagtgc ggtgtgcccg
2401 gcctcatttc tgatagatcc cgcttggggg aggtggtgta tggttacgga gctgtgcatc
2461 ttgggacatg tagtagccca ggtcggcttg tcactcgctg tgagatgggg agattttgtc
2521 ttttgattta gccccagggt tagagtgggc agggcagagc cgcgcagcac ctgggagcgg
2581 tacctttccc ttgggcagcc tggggtccca ggaacaagcc agggcgagtg gcatgtctgc
2641 ctgagcaggg tgtggcccca gaaagctgag gagtgtgggc tggcagagag cttcgagggc
2701 aaggccaccc gcgggggcgt gtgtgtggtg gggcttggca tgtgatggca gctccaggca
2761 tgccgctgct tgtatggctt tctttggcct ctgaccctgc tgcccattct ttccaacatc
2821 acagatgaac tgcctctcct cctccctgcc tggggagccc agtggccagg gaggggagtg
2881 gtggagccag tcgctgtaac actgagcctc agagacgaac caaaaccagc tgggctgagc
2941 tcagatccag ggggaaatgc tggaagtcaa taaaactgag ttttgagagc ttgaaaaaaa
3001 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//