LOCUS BC011381 2466 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens cystathionine-beta-synthase, mRNA (cDNA clone
MGC:17143 IMAGE:3869615), complete cds.
ACCESSION BC011381
VERSION BC011381.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2466)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2466)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 13 Row: i Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4557414.
FEATURES Location/Qualifiers
source 1..2466
/db_xref="H-InvDB:HIT000035232"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:17143 IMAGE:3869615"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2466
/gene="CBS"
/gene_synonym="HIP4"
/db_xref="GeneID:875"
/db_xref="MIM:236200"
CDS 109..1764
/gene="CBS"
/gene_synonym="HIP4"
/codon_start=1
/product="CBS protein"
/protein_id="AAH11381.1"
/db_xref="GeneID:875"
/db_xref="MIM:236200"
/translation="MPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWI
RPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFGLK
CELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIEPTSGNTGIGLALAAAV
RGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSH
ILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCR
IIGVDPEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTF
ARMLIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWML
QKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVD
EAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHF
ALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK"
misc_feature 334..1260
/gene="CBS"
/gene_synonym="HIP4"
/note="CysK; Region: Cysteine synthase [Amino acid
transport and metabolism]"
/db_xref="CDD:COG0031"
misc_feature 1372..1515
/gene="CBS"
/gene_synonym="HIP4"
/note="Region: Domain in cystathionine beta-synthase and
other proteins. Domain present in all 3 forms of cellular
life. Present in two copies in inosine monophosphate
dehydrogenase, of which one is disordered in the crystal
structure. A number of disease states are associated with
CBS-containing proteins including homocystinuria, Becker's
and Thomsen disease"
/db_xref="CDD:smart00116"
BASE COUNT 550 a 686 c 757 g 473 t
ORIGIN
1 ccgtctccgc ctcgccgcag tcggggcagc cgctcgcccc tcttttccat gtatccgtcc
61 agagtttgag cggtgctgaa ctgtcagcac catctgtccg gtcccagcat gccttctgag
121 accccccagg cagaagtggg gcccacaggc tgcccccacc gctcagggcc acactcggcg
181 aaggggagcc tggagaaggg gtccccagag gataaggaag ccaaggagcc cctgtggatc
241 cggcccgatg ctccgagcag gtgcacctgg cagctgggcc ggcctgcctc cgagtcccca
301 catcaccaca ctgccccggc aaaatctcca aaaatcttgc cagatattct gaagaaaatc
361 ggggacaccc ctatggtcag aatcaacaag attgggaaga agttcggcct gaagtgtgag
421 ctcttggcca agtgtgagtt cttcaacgcg ggcgggagcg tgaaggaccg catcagcctg
481 cggatgattg aggatgctga gcgcgacggg acgctgaagc ccggggacac gattatcgag
541 ccgacatccg ggaacaccgg gatcgggctg gccctggctg cggcagtgag gggctatcgc
601 tgcatcatcg tgatgccaga gaagatgagc tccgagaagg tggacgtgct gcgggcactg
661 ggggctgaga ttgtgaggac gcccaccaat gccaggttcg actccccgga gtcacacgtg
721 ggggtggcct ggcggctgaa gaacgaaatc cccaattctc acatcctaga ccagtaccgc
781 aacgccagca accccctggc tcactacgac accaccgctg atgagatcct gcagcagtgt
841 gatgggaagc tggacatgct ggtggcttca gtgggcacgg gcggcaccat cacgggcatt
901 gccaggaagc tgaaggagaa gtgtcctgga tgcaggatca ttggggtgga tcccgaaggg
961 tccatcctcg cagagccgga ggagctgaac cagacggagc agacaaccta cgaggtggaa
1021 gggatcggct acgacttcat ccccacggtg ctggacagga cggtggtgga caagtggttc
1081 aagagcaacg atgaggaggc gttcaccttt gcccgcatgc tgatcgcgca agaggggctg
1141 ctgtgcggtg gcagtgctgg cagcacggtg gcggtggccg tgaaggctgc gcaggagctg
1201 caggagggcc agcgctgcgt ggtcattctg cccgactcag tgcggaacta catgaccaag
1261 ttcctgagcg acaggtggat gctgcagaag ggctttctga aggaggagga cctcacggag
1321 aagaagccct ggtggtggca cctccgtgtt caggagctgg gcctgtcagc cccgctgacc
1381 gtgctcccga ccatcacctg tgggcacacc atcgagatcc tccgggagaa gggcttcgac
1441 caggcgcccg tggtggatga ggcgggggta atcctgggaa tggtgacgct tgggaacatg
1501 ctctcgtccc tgcttgccgg gaaggtgcag ccgtcagacc aagttggcaa agtcatctac
1561 aagcagttca aacagatccg cctcacggac acgctgggca ggctctcgca catcctggag
1621 atggaccact tcgccctggt ggtgcacgag cagatccagt accacagcac cgggaagtcc
1681 agtcagcggc agatggtgtt cggggtggtc accgccattg acttgctgaa cttcgtggcc
1741 gcccaggagc gggaccagaa gtgaagtccg gagcgctggg cggtgcggag cgggcccgcc
1801 acccttgccc acttctcctt cgctttcctg agccctaaac acacgcgtga ttggtaactg
1861 cctggcctgg caccgttatc cctgcacacg gcacagagca tccgtctccc ctcgttaaca
1921 catggcttcc taaatggccc tgtttacggc ctatgagatg aaatatgtga ttttctctaa
1981 tgtaacttcc tcttaggatg tttcaccaag gaaatattga gagagaagtc ggccaggtag
2041 gatgaacaca ggcaatgact gcgcagagtg gattaaaggc aaaagagaga agagtccagg
2101 aaggggcggg gagaagcctg ggtggctcag catcctccac gggctgcgcc gtctgctcgg
2161 ggctgagctg gcgggagcag tttgcgtgtt tgggtttttt aattgagatg aaattcaaat
2221 aacctaaaaa tcaatcactt gaaagtgaac aatcagcggc atttagtaca tccagaaagt
2281 tgtgtaggca ccacctctgt cacgttccgg aacattctgt catcaccctg tgaagcaatc
2341 atttcccctc ccgtcttcct cctcccctgg caactgctga tcgactttgt gtctctgttg
2401 tctaaaatag gttttccctg ttctggacat ttcatataaa tggaatcaca caaaaaaaaa
2461 aaaaaa
//