LOCUS BC011179 1963 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens amylase, alpha 2B (pancreatic), mRNA (cDNA clone
MGC:17195 IMAGE:4150646), complete cds.
ACCESSION BC011179
VERSION BC011179.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1963)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1963)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 19 Row: m Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21359830.
FEATURES Location/Qualifiers
source 1..1963
/db_xref="H-InvDB:HIT000035191"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:17195 IMAGE:4150646"
/tissue_type="Brain, glioblastoma with EGFR amplification"
/clone_lib="NCI_CGAP_Brn64"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1963
/gene="AMY2B"
/db_xref="GeneID:280"
/db_xref="HGNC:HGNC:478"
/db_xref="MIM:104660"
CDS 365..1900
/gene="AMY2B"
/codon_start=1
/product="amylase, alpha 2B (pancreatic)"
/protein_id="AAH11179.1"
/db_xref="GeneID:280"
/db_xref="HGNC:HGNC:478"
/db_xref="MIM:104660"
/translation="MKFFLLLFTIGFCWAQYSPNTQQGRTSIVHLFEWRWVDIALECE
RYLAPKGFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCN
NVGVRIYVDAVINHMSGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGS
GDIENYNDATQVRDCRLVGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMW
PGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAK
LGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDAR
LYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNGNDVNDWVGPPNNNGVIKEVTINPDTT
CGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWT
FSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAE
SKL"
BASE COUNT 594 a 336 c 447 g 586 t
ORIGIN
1 aaatggttca gctcttagtt atttttaaac taatgacctg tgttatactt cctattttta
61 atgggctttt atgatgtttt aggttttttt gaatcccgtg tccttcaagt gctttctaac
121 tttgagagga agaaattgac cacctggact atggaacttt ggttgaatct ggcgatgtgg
181 aacccatgga tatggagggc tgactatata caccatgttt tttcctggca catcactcaa
241 ctactattag ctggcgagtc aactcacgtt gttggaatga ctagggacaa ctagacttca
301 agtgatcttt catcccagga atataaatag tttctggaaa ggacactgac aacttcaaag
361 caaaatgaag ttctttctgt tgcttttcac cattgggttc tgctgggctc agtattcccc
421 aaatacacaa caaggacgga catctattgt tcatctgttt gaatggcgat gggttgatat
481 tgctcttgaa tgtgagcgat atttagctcc caagggattt ggaggggttc aggtctctcc
541 accaaatgaa aatgttgcaa ttcacaaccc tttcagacct tggtgggaaa gataccaacc
601 agttagctat aaattatgca caagatctgg aaatgaagat gaatttagaa acatggtgac
661 tagatgtaac aatgttgggg ttcgtattta tgtggatgct gtaattaatc atatgtctgg
721 taatgctgtg agtgcaggaa caagcagtac ctgtggaagt tacttcaacc ctggaagtag
781 ggactttcca gcagtcccat attctggatg ggattttaat gatggtaaat gtaaaactgg
841 aagtggagat atcgagaact acaatgatgc tactcaggtc agagattgtc gtctggttgg
901 tcttcttgat cttgcactgg agaaagatta tgtgcgttcc aagattgccg aatatatgaa
961 tcatctcatt gacattggtg ttgcagggtt cagacttgat gcttccaagc acatgtggcc
1021 tggagacata aaggcaattt tggacaaact gcataatcta aacagtaact ggttccctgc
1081 aggaagtaaa cctttcattt accaggaggt aattgatctg ggtggtgagc caattaaaag
1141 cagtgactac tttggaaatg gccgggtgac agaattcaag tatggtgcaa aactcggcac
1201 agttattcgc aagtggaatg gagagaagat gtcttaccta aagaactggg gagaaggttg
1261 gggtttcatg ccttctgaca gagcacttgt ctttgtggat aaccatgaca atcaacgagg
1321 acatggggct ggaggagcct ctattcttac cttctgggat gctaggctgt ataaaatggc
1381 agttggattt atgcttgctc atccttatgg ttttacacga gtaatgtcaa gctaccgttg
1441 gccaagacag tttcaaaatg gaaacgatgt taatgattgg gttgggccac caaataataa
1501 tggagtaatt aaagaagtta ctattaatcc agacactact tgtggcaatg actgggtctg
1561 tgaacatcga tggcgccaaa taaggaacat ggttaatttc cgcaatgtag tggatggcca
1621 gccttttaca aactggtatg ataatgggag caaccaagtg gcttttggga gaggaaacag
1681 aggattcatt gttttcaaca atgatgactg gacattttct ttaactttgc aaactggtct
1741 tcctgctggc acatactgtg atgtcatttc tggagataaa attaatggca attgcacagg
1801 cattaaaatc tacgtttctg acgatggcaa agctcatttt tctattagta actctgctga
1861 ggatccattt attgcaattc atgctgaatc taaattataa aatttaaaat taaatgcata
1921 tcctcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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