LOCUS BC010648 1968 bp mRNA linear HUM 30-JAN-2008
DEFINITION Homo sapiens BRF2, subunit of RNA polymerase III transcription
initiation factor, BRF1-like, mRNA (cDNA clone MGC:9916
IMAGE:3871326), complete cds.
ACCESSION BC010648
VERSION BC010648.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1968)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1968)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 13 Row: k Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 22035561.
FEATURES Location/Qualifiers
source 1..1968
/db_xref="H-InvDB:HIT000035031"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9916 IMAGE:3871326"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1968
/gene="BRF2"
/gene_synonym="BRFU"
/gene_synonym="TFIIIB50"
/db_xref="GeneID:55290"
/db_xref="HGNC:HGNC:17298"
/db_xref="MIM:607013"
CDS 84..1343
/gene="BRF2"
/gene_synonym="BRFU"
/gene_synonym="TFIIIB50"
/codon_start=1
/product="BRF2, subunit of RNA polymerase III
transcription initiation factor, BRF1-like"
/protein_id="AAH10648.1"
/db_xref="GeneID:55290"
/db_xref="HGNC:HGNC:17298"
/db_xref="MIM:607013"
/translation="MPGRGRCPDCGSTELVEDSHYSQSQLVCSDCGCVVTEGVLTTTF
SDEGNLREVTYSRSTGENEQVSRSQQRGLRRVRDLCRVLQLPPTFEDTAVAYYQQAYR
HSGIRAARLQKKEVLVGCCVLITCRQHNWPLTMGAICTLLYADLDVFSSTYMQIVKLL
GLDVPSLCLAELVKTYCSSFKLFQASPSVPAKYVEDKEKMLSRTMQLVELANETWLVT
GRHPLPVITAATFLAWQSLQPADRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRM
AEQLAWLRVLRLDKRSVVKHIGDLLQHRQSLVRSAFRDGTAEVETREKEPPGWGQGQG
EGEVGNNSLGLPQGKRPASPALLLPPCMLKSPKRICPVPPVSTVTGDENISDSEIEQY
LRTPQEVRDFQRAQAARQAATSVPNPP"
BASE COUNT 449 a 490 c 565 g 464 t
ORIGIN
1 ctcctacttc ttgctgcctc acggttgctg ttgcagaccc aggagtctct gaaggcttcc
61 gcggctttgg ggccggttgt gagatgccag gcagaggccg ctgcccggac tgcggctcca
121 cggagctggt ggaagactcg cactattcgc agagccagct ggtgtgctcc gactgcggct
181 gcgtggtcac cgagggggtc cttaccacta ccttcagcga cgagggcaat ctccgagagg
241 taacatattc ccgaagcaca ggggaaaacg aacaagttag tcgcagccag caacgaggtc
301 tccggcgagt gagagacctt tgtcgagttc tgcagttgcc accaacattt gaggataccg
361 cggttgccta ctaccaacag gcatatcggc actctggcat ccgagcggcc aggctgcaaa
421 agaaggaggt gttggttggg tgctgcgtct taatcacctg ccgacagcat aactggcccc
481 taacaatggg ggccatctgc acgctgttgt atgcagattt ggatgtgttt tctagcactt
541 acatgcagat agtgaagctc ctgggactgg atgtgccatc tctgtgcttg gcagaactgg
601 tgaagaccta ttgcagcagc ttcaaactgt tccaagcttc accttctgtg ccagccaaat
661 acgtggaaga caaagagaag atgctgtctc gaacaatgca gttggtggag ctggcaaatg
721 agacgtggct ggtgaccggg aggcatccct tgcccgtcat cactgctgcg actttcctgg
781 cttggcagtc gctgcagcct gcagatcggc tttcatgttc ccttgcccga ttttgtaaat
841 tggcaaatgt ggacctgccc tacccggcgt cctcccgcct gcaggagctg ctggctgtgc
901 tgctgcggat ggctgagcag ctggcctggt tacgagttct gagacttgac aaacggtctg
961 tggtgaagca catcggtgac cttctccagc accgccagtc actggtccgc tctgcctttc
1021 gggatgggac agcagaagtg gagacccgag agaaggagcc accggggtgg ggacaggggc
1081 aaggagaagg ggaggtggga aataattcct taggtttacc ccaggggaag cggccggcca
1141 gtcctgccct tctcttgcca ccctgcatgt tgaagtcccc gaagcggatc tgccctgtac
1201 cccctgtctc cactgtcact ggagatgaga acatttctga tagtgaaata gaacagtatt
1261 tgcgtacccc tcaggaagtt agggactttc agagagccca ggctgctaga caggctgcca
1321 cgagtgtccc taaccctccc tgatggatat ccactgggag cacttcatcc tgttctgaca
1381 gcttgataac attcctgtta taaccaagga tggaagtgta caccagtccg taggtattgc
1441 ttttcttgtt tgaaggaacc aagaggggct ctgccattag ttggaccctg ggtcctggag
1501 taaagtcagg agtgcaggga tgactatagg taggagagat tcccatccct tggtggtggg
1561 agagcaagtt gcctatgtcc atgttctgtg agatggcttt cctcatagat ggatgggaaa
1621 atgtcaggct ctttgctgct ggtttgaatt ggacacactg ctgcggctcc tcctgcaggc
1681 ctgagggggc ttccctctgc ttgtggagtg gttggcattc ccagcagtat caaccctcag
1741 aggagcggga actggggaat tctggcccta cgtgcattca caggcaatga tgggtttgtg
1801 tgtatggtgt catgagatcc tctacctcat aacaaaagga cagtgggtag actaaggcag
1861 tagctcaaag ggctttgcaa aattttaata tattaaaaca agaggcatct gctagaaaac
1921 attctattgt ataaaacccg agttcttaaa aaaaaaaaaa aaaaaaaa
//