LOCUS BC010037 1410 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens glutamate-ammonia ligase (glutamine synthetase), mRNA
(cDNA clone MGC:19730 IMAGE:3545927), complete cds.
ACCESSION BC010037
VERSION BC010037.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1410)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1410)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC010037.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 27 Row: e Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361767.
FEATURES Location/Qualifiers
source 1..1410
/db_xref="H-InvDB:HIT000034756"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19730 IMAGE:3545927"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1410
/gene="GLUL"
/gene_synonym="GS"
/gene_synonym="PIG43"
/db_xref="GeneID:2752"
/db_xref="HGNC:HGNC:4341"
/db_xref="MIM:138290"
CDS 114..1235
/gene="GLUL"
/gene_synonym="GS"
/gene_synonym="PIG43"
/codon_start=1
/product="glutamate-ammonia ligase (glutamine synthetase)"
/protein_id="AAH10037.1"
/db_xref="GeneID:2752"
/db_xref="HGNC:HGNC:4341"
/db_xref="MIM:138290"
/translation="MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKT
RTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVF
KYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGP
YYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHL
WVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEK
LSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKK
GYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN"
BASE COUNT 367 a 374 c 358 g 311 t
ORIGIN
1 gggagaagag cggagcgtgt gagcagtact gcggcctcct ctcctctcct aacctcgctc
61 tcgcggccta cctttacccg cccgcctgct cggcgaccag aacaccttcc accatgacca
121 cctcagcaag ttcccactta aataaaggca tcaagcaggt gtacatgtcc ctgcctcagg
181 gtgagaaagt ccaggccatg tatatctgga tcgatggtac tggagaagga ctgcgctgca
241 agacccggac cctggacagt gagcccaagt gtgtggaaga gttgcctgag tggaatttcg
301 atggctccag tactttacag tctgagggtt ccaacagtga catgtatctc gtgcctgctg
361 ccatgtttcg ggaccccttc cgtaaggacc ctaacaagct ggtgttatgt gaagttttca
421 agtacaatcg aaggcctgca gagaccaatt tgaggcacac ctgtaaacgg ataatggaca
481 tggtgagcaa ccagcacccc tggtttggca tggagcagga gtataccctc atggggacag
541 atgggcaccc ctttggttgg ccttccaacg gcttcccagg gccccagggt ccatattact
601 gtggtgtggg agcagacaga gcctatggca gggacatcgt ggaggcccat taccgggcct
661 gcttgtatgc tggagtcaag attgcgggga ctaatgccga ggtcatgcct gcccagtggg
721 aatttcagat tggaccttgt gaaggaatca gcatgggaga tcatctctgg gtggcccgtt
781 tcatcttgca tcgtgtgtgt gaagactttg gagtgatagc aacctttgat cctaagccca
841 ttcctgggaa ctggaatggt gcaggctgcc ataccaactt cagcaccaag gccatgcggg
901 aggagaatgg tctgaagtac atcgaggagg ccattgagaa actaagcaag cggcaccagt
961 accacatccg tgcctatgat cccaagggag gcctggacaa tgcccgacgt ctaactggat
1021 tccatgaaac ctccaacatc aacgactttt ctgctggtgt agccaatcgt agcgccagca
1081 tacgcattcc ccggactgtt ggccaggaga agaagggtta ctttgaagat cgtcgcccct
1141 ctgccaactg cgaccccttt tcggtgacag aagccctcat ccgcacgtgt cttctcaatg
1201 aaaccggcga tgagcccttc cagtacaaaa attaagtgga ctagacctcc agctgttgag
1261 cccctcctag ttcttcatcc cactccaact cttccccctc tcccagttgt cccgattgta
1321 actcaaaggg tggaatatca aggtcgtttt tttcattcca aaaaaaaaaa aaaaaaaaaa
1381 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//