LOCUS BC009872 1787 bp mRNA linear HUM 24-JAN-2005
DEFINITION Homo sapiens branched chain ketoacid dehydrogenase kinase, mRNA
(cDNA clone MGC:16430 IMAGE:3944953), complete cds.
ACCESSION BC009872
VERSION BC009872.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1787)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1787)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC009872.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 25 Row: g Column: 23.
FEATURES Location/Qualifiers
source 1..1787
/db_xref="H-InvDB:HIT000034633"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:16430 IMAGE:3944953"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1787
/gene="BCKDK"
/db_xref="GeneID:10295"
CDS 227..1234
/gene="BCKDK"
/codon_start=1
/product="BCKDK protein"
/protein_id="AAH09872.1"
/db_xref="GeneID:10295"
/translation="MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMAR
ERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRI
AHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLD
DHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFV
GIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPEL
LKNAMRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMH
G"
BASE COUNT 352 a 601 c 494 g 340 t
ORIGIN
1 actgttcgag cccttccgct gggacccggg ccctggctcc ggccccgcga tgggagctgc
61 tctccgcggg ctgagcctgt cagcatcctc gacgcaccct ggtccctgaa gtcggagaag
121 agcccctacc cacccacacc cccttgccca attttgggtc gcctgggtcc tcagtcctag
181 cggatcctca gtcctagcgg ccaccgggtc tgaaaggagc aagacgatga tcctggcgtc
241 ggtgctgagg agcggtcccg ggggcgggct tccgctccgg cccctcctgg gacccgcact
301 cgcgctccgg gcccgctcga cgtcggccac cgacacacac cacgtggaga tggctcggga
361 gcgctccaag accgtcacct ccttttacaa ccagtcggcc atcgacgcgg cagcggagaa
421 gccctcagtc cgcctaacgc ccaccatgat gctctacgct ggccgctctc aggacggcag
481 ccaccttctg aaaagtgctc ggtacctgca gcaagaactt ccagtgagga ttgctcaccg
541 catcaagggc ttccgctgcc ttcctttcat cattggctgc aaccccacca tactgcacgt
601 gcatgagcta tatatccgtg ccttccagaa gctgacagac ttccctccga tcaaggacca
661 ggcggacgag gcccagtact gccagctggt gcgacagctg ctggatgacc acaaggatgt
721 ggtgaccctc ttggcagagg gcctacgtga gagccggaag cacatagagg atgaaaagct
781 cgtccgctac ttcttggaca agacgctgac ttcgaggctt ggaatccgca tgttggccac
841 gcatcacctg gcgctgcatg aggacaagcc tgactttgtc ggcatcatct gtactcgtct
901 ctcaccaaag aagattattg agaagtgggt ggactttgcc agacgcctgt gtgagcacaa
961 gtatggcaat gcgccccgtg tccgcatcaa tggccatgtg gctgcccggt tccccttcat
1021 ccctatgcca ctggactaca tcctgccgga gctgctcaag aatgccatga ggatctcaga
1081 ccgtggtgga ggaatcgctc acaaagatct ggaccgggtc atggactacc acttcactac
1141 tgctgaggcc agcacacagg acccccggat cagccccctc tttggccatc tggacatgca
1201 tagtggcgcc cagtcaggac ccatgcacgg gtgagaccct gccaggccag gatggagggg
1261 tgggggaccc caggagactc aagcctctga agcctcctgt cctgtccccc tgcccacccc
1321 cagctttggc ttcgggttgc ccacgtcacg ggcctacgcg gagtacctcg gtgggtctct
1381 gcagctgcag tccctgcagg gcattggcac ggacgtctac ctgcggctcc gccacatcga
1441 tggccgggag gaaagcttcc ggatctgacc ccacagcctt tggcctgctc acccgaccag
1501 cctgggccgc attccctgca ggacctcccg ggtcaggcag ggcggccccc tgctccacac
1561 actgctgcat cttgggtctc agggacccag acagatggac ttacatggag ctgggcactg
1621 ccctgcctca acagggtcca ttgcctcctc gcctccagaa cttggagcag ggaagtgggc
1681 accctgaggc ctccagcacc agttccgtca ttctcgttcc tggggaaccc ccactctgac
1741 ctgttattaa agttcacatt ttgaatgcca aaaaaaaaaa aaaaaaa
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