LOCUS BC009701 1626 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens peptidyl arginine deiminase, type II, mRNA (cDNA clone
MGC:9250 IMAGE:3901790), complete cds.
ACCESSION BC009701
VERSION BC009701.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1626)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1626)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (29-JUN-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 14 Row: o Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 15042936.
FEATURES Location/Qualifiers
source 1..1626
/db_xref="H-InvDB:HIT000034515"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9250 IMAGE:3901790"
/tissue_type="Pancreas, epithelioid carcinoma"
/clone_lib="NIH_MGC_70"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1626
/gene="PADI2"
/gene_synonym="KIAA0994"
/gene_synonym="PDI2"
/db_xref="GeneID:11240"
/db_xref="MIM:607935"
CDS 65..1378
/gene="PADI2"
/gene_synonym="KIAA0994"
/gene_synonym="PDI2"
/codon_start=1
/product="PADI2 protein"
/protein_id="AAH09701.1"
/db_xref="GeneID:11240"
/db_xref="MIM:607935"
/translation="MLRERTVRLQYGSRVEAVYVLGTYLWTDVYSAAPAGAQTFSLKH
SEHVWVEVVRDGEAEEVATNGKQRWLLSPSTTLRVTMSQASTEASSDKVTVNYYDEEG
SIPIDQAGLFLTAIEISLDVDADRDGVVEKNNPKKASWTWGPEGQGAILLVNCDRETP
WLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVE
NPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYM
AQDIPLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCE
LKVCFQYLNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGY
VTREPLFESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPL"
misc_feature 65..1375
/gene="PADI2"
/gene_synonym="KIAA0994"
/gene_synonym="PDI2"
/note="PAD; Region: Protein-arginine deiminase (PAD).
Members of this family are found in mammals. In the
presence of calcium ions, PAD enzymes EC:3.5.3.15 catalyse
the post-translational modification reaction responsible
for the formation of citrulline residues: Protein
L-arginine + H2O <=> Protein L-citrulline + NH3. Several
types are recognized (and included in the family) on the
basis of molecular mass, substrate specificity, and tissue
localization. The expression of type I PAD is known to be
under the control of oestrogen"
/db_xref="CDD:pfam03068"
BASE COUNT 359 a 456 c 481 g 330 t
ORIGIN
1 cgcacctgct gcaggtgctc ccggccgccc cggaccagcg agcgcgggca ctgcggcggg
61 gaggatgctg cgcgagcgga ccgtgcggct gcagtacggg agccgcgtgg aggcggtgta
121 cgtgctgggc acctacctct ggaccgatgt ctacagcgcg gccccagccg gggcccaaac
181 cttcagcctg aagcactcgg aacacgtgtg ggtggaggtg gtgcgtgatg gggaggctga
241 ggaggtggcc accaatggca agcagcgctg gcttctctcg cccagcacca ccctgcgggt
301 caccatgagc caggcgagca ccgaggccag cagtgacaag gtcaccgtca actactatga
361 cgaggaaggg agcattccca tcgaccaggc ggggctcttc ctcacagcca ttgagatctc
421 cctggatgtg gacgcagacc gggatggtgt ggtggagaag aacaacccaa agaaggcatc
481 ctggacctgg ggccccgagg gccagggggc catcctgctg gtgaactgtg accgagagac
541 accctggttg cccaaggagg actgccgtga tgagaaggtc tacagcaagg aagatctcaa
601 ggacatgtcc cagatgatcc tgcggaccaa aggccccgac cgcctccccg ccggatacga
661 gatagttctg tacatttcca tgtcagactc agacaaagtg ggcgtgttct acgtggagaa
721 cccgttcttc ggccaacgct atatccacat cctgggccgg cggaagctct accatgtggt
781 caagtacacg ggtggctccg cggagctgct gttcttcgtg gaaggcctct gtttccccga
841 cgagggcttc tcaggcctgg tctccatcca tgtcagcctg ctggagtaca tggcccagga
901 cattcccctg actcccatct tcacggacac cgtgatattc cggattgctc cgtggatcat
961 gacccccaac atcctgcctc ccgtgtcggt gtttgtgtgc tgcatgaagg ataattacct
1021 gttcctgaaa gaggtgaaga accttgtgga gaaaaccaac tgtgagctga aggtctgctt
1081 ccagtaccta aaccgaggcg atcgctggat ccaggatgaa attgagtttg gctacatcga
1141 ggccccccat aaaggcttcc ccgtggtgct ggactctccc cgagatggaa acctaaagga
1201 cttccctgtg aaggagctcc tgggcccaga ttttggctac gtgacccggg agcccctctt
1261 tgagtctgtc accagccttg actcatttgg aaacctggag gtcagtcccc cagtgaccgt
1321 gaatggcaag acatacccgc ttggccgcat cctcatcggg agcagctttc ctctgtaaga
1381 gaagccaggc tggggctagg ggctgtggga gtggggaagt cactgtttct cttttggggt
1441 ggcctgggtt gctcacacat ggagcaagtg gctgggggaa ttattccctc ccacgacttc
1501 ctgttaagag gccgacactc ttggaaagaa aatcaagcta gcctttatat ctttgtgtga
1561 tttaggatct aatatataac taaataaaca actcttttcc ccaagtgaaa aaaaaaaaaa
1621 aaaaaa
//