LOCUS BC009350 3016 bp mRNA linear HUM 03-AUG-2005
DEFINITION Homo sapiens eukaryotic translation initiation factor 2 alpha
kinase 4, mRNA (cDNA clone IMAGE:4137621), partial cds.
ACCESSION BC009350
VERSION BC009350.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3016)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3016)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-JUN-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 9, 2003 this sequence version replaced BC009350.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 26 Row: m Column: 6.
FEATURES Location/Qualifiers
source 1..3016
/db_xref="H-InvDB:HIT000034299"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4137621"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..3016
/gene="EIF2AK4"
/gene_synonym="GCN2"
/gene_synonym="KIAA1338"
/db_xref="GeneID:440275"
/db_xref="MIM:609280"
CDS <1..2492
/gene="EIF2AK4"
/gene_synonym="GCN2"
/gene_synonym="KIAA1338"
/codon_start=3
/product="EIF2AK4 protein"
/protein_id="AAH09350.2"
/db_xref="GeneID:440275"
/db_xref="MIM:609280"
/translation="DTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKI
GDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQ
KVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISW
LLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQR
ISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIY
EHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPK
ELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLH
CGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDL
MPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLCIKLQVLINLGLVYKVQQHNGII
FQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVL
NMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCR
HHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGRE
ASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQVQTRLQT
SLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKL
VCDEIYNIKVEKKVSVLFLYSYRDDYYRILF"
BASE COUNT 927 a 649 c 672 g 768 t
ORIGIN
1 gagacaccgt cagactctgg aggctttttc gagagattct ggatggatta gcttatatcc
61 atgagaaagg aatgattcac cgggatttga agcctgtcaa catttttttg gattctgatg
121 accatgtgaa aataggtgat tttggtttgg cgacagacca tctagccttt tctgctgaca
181 gcaaacaaga cgatcagaca ggagacttga ttaagtcaga cccttcaggt cacttaactg
241 ggatggttgg cactgctctc tatgtaagcc cagaggtcca aggaagcacc aaatctgcat
301 acaaccagaa agtggatctc ttcagcctgg gaattatctt ctttgagatg tcctatcacc
361 ccatggtcac ggcttcagaa aggatctttg ttctcaacca actcagagat cccacttcgc
421 ctaagtttcc agaagacttt gacgatggag agcatgcaaa gcagaaatca gtcatctcct
481 ggctgttgaa ccacgatcca gcaaaacggc ccacagccac agaactgctc aagagtgagc
541 tgctgccccc accccagatg gaggagtcag agctgcatga agtgctgcac cacacgctga
601 ccaacgtgga tgggaaggcc taccgcacca tgatggccca gatcttctcg cagcgcatct
661 cccctgccat cgattacacc tatgacagcg acatactgaa gggcaacttc tcaatccgta
721 cagccaagat gcagcagcat gtgtgtgaaa ccatcatccg catctttaaa agacatggag
781 ctgttcagtt gtgtactcca ctactgcttc cccgaaacag acaaatatat gagcacaacg
841 aagctgccct attcatggac cacagcggga tgctggtgat gcttcctttt gacctgcgga
901 tcccttttgc aagatatgtg gcaagaaata atatattgaa tttaaaacga tactgcatag
961 aacgtgtgtt caggccgcgc aagttagatc gatttcatcc caaagaactt ctggagtgtg
1021 catttgatat tgtcacttct accaccaaca gctttctgcc cactgctgaa attatctaca
1081 ctatctatga aatcatccaa gagtttccag cacttcagga aagaaattac agtatttatt
1141 tgaaccatac catgttattg aaagcaatac tcttacactg tgggatccca gaagataaac
1201 tcagtcaagt ctacattatt ctgtatgatg ctgtgacaga gaagctgacg aggagagaag
1261 tggaagctaa attttgtaat ctgtctttgt cttctaatag tctgtgtcga ctctacaagt
1321 ttattgaaca gaagggagat ttgcaagatc ttatgccaac aataaattca ttaataaaac
1381 agaaaacagg tattgcacag ttggtgaagt atggcttaaa agacctagag gaggttgttg
1441 gactgttgaa gaaactctgc atcaagttac aggtcttgat caatttgggc ttggtttaca
1501 aggtgcagca gcacaatgga atcatcttcc agtttgtggc tttcatcaaa cgaaggcaaa
1561 gggctgtacc tgaaatcctc gcagctggag gcagatatga cctgctgatt ccccagttta
1621 gagggccaca agctctgggg ccagttccca ctgccattgg ggtcagcata gctatagaca
1681 agatatctgc tgctgtcctc aacatggagg aatctgttac aataagctct tgtgacctcc
1741 tggttgtaag tgttggccag atgtctatgt ccagggccat caacctaacc cagaaactct
1801 ggacagcagg catcacagca gaaatcatgt acgactggtc acagtcccaa gaggaattac
1861 aagagtactg cagacatcat gaaatcacct atgtggccct tgtctcggat aaagaaggaa
1921 gccatgtcaa ggttaagtct ttcgagaagg aaaggcagac agagaagcgt gtgctggaga
1981 ctgaacttgt ggaccatgta ctgcagaaac tgaggactaa agtcactgat gaaaggaatg
2041 gcagagaagc ttccgataat cttgcagtgc aaaatctgaa ggggtcattt tctaatgctt
2101 caggtttgtt tgaaatccat ggagcaacag tggttcccat tgtgagtgtg ctagccccgg
2161 agaagctgtc agccagcact aggaggcgct atgaaactca ggtacaaact cgacttcaga
2221 cctcccttgc caacttacat cagaaaagca gtgaaattga aattctggct gtggatctac
2281 ccaaagaaac aatattacag tttttatcat tagagtggga tgctgatgaa caggcattta
2341 acacaactgt gaagcagctg ctgtcacgcc tgccaaagca aagatacctc aaattagtct
2401 gtgatgaaat ttataacatc aaagtagaaa aaaaggtgtc tgtgctattt ctgtacagct
2461 atagagatga ctactacaga atcttatttt aaccctaaag aactgtcgtt aacctcattc
2521 aaacagacag aggcttatac tggaataatg gaatgttgta cattcatcat aatttaaaat
2581 taaattctaa gaagaggctg ggtgcagtgg ctcacacctt taatcccagc actttgggaa
2641 gccaaggcag gaagactgct tgaaaccagg agtttgagac cagcctgagc aacaaagcaa
2701 gaccccatct ctataaaaac taaaaaaatt agttgggcat ggtggcacat gcctgtagtc
2761 ccagctactc cagaggctga gatggatcat ctgagcctca ggaggttgag gctgcagtga
2821 gctgtgactg cgccactgca ctccagtctg ggacaacaga gcaagaccct gtcttaaaaa
2881 aaaaaagaaa aaaaaaattt ttttgtaaga agctgtccta caaagttgag ctttgttagt
2941 ttttcatgtg taatatatta taaatttatc ttttgggata taataaatgc tttcatataa
3001 aaaaaaaaaa aaaaaa
//