LOCUS BC009139 1454 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog,
yeast), mRNA (cDNA clone MGC:9268 IMAGE:3853408), complete cds.
ACCESSION BC009139
VERSION BC009139.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1454)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1454)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-JUN-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 13 Row: a Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33359690.
FEATURES Location/Qualifiers
source 1..1454
/db_xref="H-InvDB:HIT000034179"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9268 IMAGE:3853408"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_65"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1454
/gene="UBE2E1"
/gene_synonym="UBCH6"
/db_xref="GeneID:7324"
/db_xref="HGNC:HGNC:12477"
/db_xref="MIM:602916"
CDS 154..735
/gene="UBE2E1"
/gene_synonym="UBCH6"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2E 1 (UBC4/5
homolog, yeast)"
/protein_id="AAH09139.1"
/db_xref="GeneID:7324"
/db_xref="HGNC:HGNC:12477"
/db_xref="MIM:602916"
/translation="MSDDDSRASTSSSSSSSSNQQTEKETNTPKKKESKVSMSKNSKL
LSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITF
TPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCN
PADPLVGSIATQYMTNRAEHDRMARQWTKRYAT"
BASE COUNT 444 a 287 c 318 g 405 t
ORIGIN
1 agttgctgtt gctgcacttc cgcttctctc ccagcgagag agagacacga gtggccaggc
61 ccagccgcag ccgcagcagc agccgccgcg gcggcacgga ggagccagac acaaagagag
121 gggctgtttg cggggtgggg tggggggttc gctatgtcgg atgacgattc gagggccagc
181 accagctcct cctcatcttc gtcttccaac cagcaaaccg agaaagaaac aaacaccccc
241 aagaagaagg agagtaaagt cagcatgagc aaaaactcca aactcctctc caccagcgcc
301 aagagaattc agaaggagct ggcggacatc actttagacc ctccacctaa ttgcagtgct
361 ggtcccaaag gcgataacat ctatgaatgg agatcaacca ttctagggcc tccaggatcc
421 gtgtatgagg gtggtgtatt ctttctcgat atcactttta caccagaata tcccttcaag
481 cctccaaagg ttacatttcg gacaagaatc tatcattgta atattaacag tcaaggtgtt
541 atttgcttgg acatattgaa agataattgg agtccagcac taaccatttc taaagtcctc
601 ctttctatct gctcacttct tacagactgt aatcctgccg accccttggt gggaagtatt
661 gccactcagt atatgaccaa cagagcagaa catgacagaa tggccagaca gtggaccaag
721 agatacgcta cataaattgg ggtttcacaa ttcttacatt atttgtctgt cacagaagag
781 agctgcttat gattttgaag gggtcaggga gggtgggagt tggtaaagag tagggtattt
841 ctataacaga tattattcag tcttatttcc taagattttg ttgtaactta aggtatcttg
901 ctacagtaga cagaattggt aatagcaact tttaaaattg tcattagttc tgcaatatta
961 gctgaaatgt agtacagaaa agaatgtaca tttagacatt tgggttcagt tgcttgtagt
1021 ctgtaaattt aaaacagctt aatttggtac aggttacaca tatggccatt tatgtaaagt
1081 ccctctaaga ctacatactt tttgtttaaa acaaaattgg aatttgtttt cccttcttgg
1141 aagggaacat tgatatttaa cagagttttt agagattgtc atctcatata aaatggacac
1201 gtggctataa aacaccatat aagagatgag tagtgcgttt tattttatat gccaatctac
1261 tttgtttaaa aaaggtctga atcaggactt gtgaaaacct gtagtgaaat accttaagct
1321 gttaactaac tgtaaggcgt ggaataggag ttgctcagtg gattggttct atgttgtgga
1381 ctacttaagt ctgcatttgt tactgtgcta ataaacaata ttaaaaacca cctaataaac
1441 aaaaaaaaaa aaaa
//