LOCUS BC008943 1465 bp mRNA linear HUM 19-NOV-2003
DEFINITION Homo sapiens PX domain containing serine/threonine kinase, mRNA
(cDNA clone IMAGE:2924122), partial cds.
ACCESSION BC008943
VERSION BC008943.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1465)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1465)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC008943.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 2 Row: m Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 23618917.
FEATURES Location/Qualifiers
source 1..1465
/db_xref="H-InvDB:HIT000087175"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:2924122"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..>1465
/gene="PXK"
/gene_synonym="FLJ20335"
/db_xref="GeneID:54899"
CDS 81..>1465
/gene="PXK"
/gene_synonym="FLJ20335"
/codon_start=1
/product="PXK protein"
/protein_id="AAH08943.1"
/db_xref="GeneID:54899"
/translation="MAFMEKPPAGKVLLDDTVPLTAAIEASQSLQSHTEYIIRVQRGI
SVENSWQIVRRYSDFDLLNNSLQIAGLSLPLPPKKLIGNMDREFIAERQKGLQNYLNV
ITTNHILSNCELVKKFLDPNNYSANYTEIALQQVSMFFRSEPKWEVVEPLKDIGWRIR
KKYFLMKIKNQPKERLVLSWADLGPDKYLSDKDFQCLIKLLPSCLHPYIYRVTFATAN
ESSALLIRMFNEKGTLKDLIYKAKPKDPFLKKYCNPKKIQGLELQQIKTYGRQILEVL
KFLHDKGFPYGHLHASNVMLDGDTCRLLDLENSLLGLPSFYRSYFSQFRKINTLESVD
VHCFGHLLYEMTYGRPPDSVPVDSFPPAPSMAVVAVLESTLSCEACKNGMPTISRLLQ
MPLFSDVLLTTSEKPQFKIPTKLKEALRIAKECIEKRLIEEQKQIHQHRRLTRAQSHH
GSEEERKKKKK"
misc_feature 174..446
/gene="PXK"
/gene_synonym="FLJ20335"
/note="PX; Region: PX domain. PX domains bind to
phosphoinositides"
/db_xref="CDD:pfam00787"
misc_feature 672..1127
/gene="PXK"
/gene_synonym="FLJ20335"
/note="S_TKc; Region: Serine/Threonine protein kinases,
catalytic domain. Phosphotransferases of the serine or
threonine-specific kinase subfamily. The enzymatic
activity of these protein kinases is controlled by
phosphorylation of specific residues in the activation
segment of the catalytic domain, sometimes combined with
reversible conformational changes in the C-terminal
autoregulatory tail"
/db_xref="CDD:cd00180"
BASE COUNT 430 a 328 c 345 g 362 t
ORIGIN
1 tggaaccggg cgggcggcgg gagtcggcgc ctcgggttcc tacctcgcgt ccctaggcgg
61 cggcggccgg gcgtcccggg atggccttca tggagaagcc gccagccggc aaggtgctgc
121 tggacgacac ggtgccgctg acagcagcca tcgaggcgag ccagagcctg cagtcccaca
181 cggaatatat tattcgagtg caaagaggaa tttctgtgga aaacagctgg cagattgtta
241 gaagatacag tgactttgat ttgcttaaca acagcttaca gattgcaggc ctaagtctac
301 ctcttcctcc caaaaaattg attggtaaca tggatcgtga attcatagct gaaaggcaga
361 aaggtcttca gaactatctc aacgtgatca caacaaatca tatcttgtct aattgtgagc
421 tggttaagaa gtttttagat ccaaacaact attccgcaaa ctatactgag attgccttgc
481 aacaggtttc catgttcttc cgatcagaac caaagtggga ggtggtggaa cctttgaaag
541 acataggttg gagaataagg aagaaatatt tcttgatgaa gattaaaaat cagccaaagg
601 aacggctagt gttaagctgg gctgaccttg gcccagacaa gtatttgtca gataaagatt
661 ttcagtgtct aatcaaactt ctgccttctt gtttgcaccc ttacatctat cgggttacct
721 ttgccacagc taatgaatcc tcagcgttgc taattaggat gtttaacgaa aagggaacat
781 tgaaggatct gatctacaag gcaaaaccaa aagacccatt tctaaagaag tactgcaacc
841 ctaagaagat tcagggcctg gaactccagc aaataaaaac atatggacgg caaatattag
901 aggtactgaa gtttcttcat gacaagggat tcccttatgg gcatcttcac gcctccaatg
961 tgatgctcga tggggacact tgtcggctgc tggaccttga gaattcctta ttgggcctgc
1021 cttccttcta ccgatcttat ttttcacaat tcaggaaaat caatacattg gaaagtgtgg
1081 atgtccactg ctttggccac ttactgtatg aaatgactta tggacgaccg ccagactcgg
1141 tgcctgtgga ctccttccct cctgccccgt ccatggctgt ggtggccgtg ttggagtcta
1201 cgctgtcttg tgaagcctgt aaaaatggca tgcctaccat ctcccggctc ttacagatgc
1261 cattattcag cgatgtttta ctaaccactt ctgaaaaacc acagtttaag atccctacaa
1321 agttaaaaga ggcattgaga attgccaaag aatgtataga gaagagacta attgaggaac
1381 agaaacagat tcaccagcat cgaagactga caagagctca gtcccaccat ggatctgagg
1441 aggaaagaaa aaaaaaaaaa aaaaa
//