LOCUS BC008723 2230 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens asparagine synthetase, mRNA (cDNA clone MGC:8639
IMAGE:2961551), complete cds.
ACCESSION BC008723
VERSION BC008723.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2230)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2230)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC008723.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: n Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34452702.
FEATURES Location/Qualifiers
source 1..2230
/db_xref="H-InvDB:HIT000033923"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8639 IMAGE:2961551"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2230
/gene="ASNS"
/gene_synonym="TS11"
/db_xref="GeneID:440"
/db_xref="HGNC:HGNC:753"
/db_xref="MIM:108370"
CDS 357..2042
/gene="ASNS"
/gene_synonym="TS11"
/codon_start=1
/product="asparagine synthetase"
/protein_id="AAH08723.1"
/db_xref="GeneID:440"
/db_xref="HGNC:HGNC:753"
/db_xref="MIM:108370"
/translation="MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCC
FGFHRLAVVDPLFGMQPIRVKKYPYLWLCYNGEIYNHKKMQQHFEFEYQTKVDGEIIL
HLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSE
AKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHALYDN
VEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKE
AQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETY
DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESER
LLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRE
TFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFN
TPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA"
BASE COUNT 644 a 473 c 509 g 604 t
ORIGIN
1 ctgacctgct tacgcccagc ctgtggacat tttcgcggtc cgggtccctg cggcggacgg
61 gtcggatcgt gctcgtggcc agggcgcagt caggtgtcgc gtggtcctga tctaggaaga
121 gactgcggcc ttatcaccgt ctcggtgtct gctttcacct tcaactgacc ccctcctcac
181 ctggaccgct caccctcctc ctcccctctc accccgccat ccccaccagt ccccacctgt
241 ctctgtagac tgcaagactc atcaagtgac ttgctgagca aaccctgccg agaagagatt
301 ttcttcaatc acatctgaat aaatcacttg aagaaagctt atagcttcat tgcaccatgt
361 gtggcatttg ggcgctgttt ggcagtgatg attgcctttc tgttcagtgt ctgagtgcta
421 tgaagattgc acacagaggt ccagatgcat tccgttttga gaatgtcaat ggatacacca
481 actgctgctt tggatttcac cggttggcgg tagttgaccc gctgtttgga atgcagccaa
541 ttcgagtgaa gaaatatccg tatttgtggc tctgttacaa tggtgaaatc tacaaccata
601 agaagatgca acagcatttt gaatttgaat accagaccaa agtggatggt gagataatcc
661 ttcatcttta tgacaaagga ggaattgagc aaacaatttg tatgttggat ggtgtgtttg
721 catttgtttt actggatact gccaataaga aagtgttcct gggtagagat acatatggag
781 tcagaccttt gtttaaagca atgacagaag atggattttt ggctgtatgt tcagaagcta
841 aaggtcttgt tacattgaag cactccgcga ctcccttttt aaaagtggag ccttttcttc
901 ctggacacta tgaagttttg gatttaaagc caaatggcaa agttgcatcc gtggaaatgg
961 ttaaatatca tcactgtcgg gatgaacccc tgcacgccct ctatgacaat gtggagaaac
1021 tctttccagg ttttgagata gaaactgtga agaacaacct caggatcctt tttaataatg
1081 ctgtaaagaa acgtttgatg acagacagaa ggattggctg ccttttatca gggggcttgg
1141 actccagctt ggttgctgcc actctgttga agcagctgaa agaagcccaa gtacagtatc
1201 ctctccagac atttgcaatt ggcatggaag acagccccga tttactggct gctagaaagg
1261 tggcagatca tattggaagt gaacattatg aagtcctttt taactctgag gaaggcattc
1321 aggctctgga tgaagtcata ttttccttgg aaacttatga cattacaaca gttcgtgctt
1381 cagtaggtat gtatttaatt tccaagtata ttcggaagaa cacagatagc gtggtgatct
1441 tctctggaga aggatcagat gaacttacgc agggttacat atattttcac aaggctcctt
1501 ctcctgaaaa agccgaggag gagagtgaga ggcttctgag ggaactctat ttgtttgatg
1561 ttctccgcgc agatcgaact actgctgccc atggtcttga actgagagtc ccatttctag
1621 atcatcgatt ttcttcctat tacttgtctc tgccaccaga aatgagaatt ccaaagaatg
1681 ggatagaaaa acatctcctg agagagacgt ttgaggattc caatctgata cccaaagaga
1741 ttctctggcg accaaaagaa gccttcagtg atggaataac ttcagttaag aattcctggt
1801 ttaagatttt acaggaatac gttgaacatc aggttgatga tgcaatgatg gcaaatgcag
1861 cccagaaatt tcccttcaat actcctaaaa ccaaagaagg atattactac cgtcaagtct
1921 ttgaacgcca ttacccaggc cgggctgact ggctgagcca ttactggatg cccaagtgga
1981 tcaatgccac tgacccttct gcccgcacgc tgacccacta caagtcagct gtcaaagctt
2041 aggtggtctt tatgctgtaa tgtgaaagca aatatttctt cgtgttggat ggggactgtg
2101 ggtagatagg ggaacaatga gagtcaactc aggctaactt gggtgtgaaa aaaataaaag
2161 tcctaaatct aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2221 aaaaaaaaaa
//