LOCUS BC008323 1424 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens creatine kinase, brain, mRNA (cDNA clone MGC:15764
IMAGE:3358085), complete cds.
ACCESSION BC008323
VERSION BC008323.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1424)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1424)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 23 Row: b Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1424
/db_xref="H-InvDB:HIT000033723"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15764 IMAGE:3358085"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_16"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1424
/gene="CKB"
/gene_synonym="B-CK"
/db_xref="GeneID:1152"
/db_xref="HGNC:HGNC:1991"
/db_xref="MIM:123280"
CDS 64..1209
/gene="CKB"
/gene_synonym="B-CK"
/codon_start=1
/product="creatine kinase, brain"
/protein_id="AAH08323.1"
/db_xref="GeneID:1152"
/db_xref="HGNC:HGNC:1991"
/db_xref="MIM:123280"
/translation="MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRA
KSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGYKP
SDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVE
ALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIW
HNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPH
LGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVF
DVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK"
BASE COUNT 295 a 471 c 425 g 233 t
ORIGIN
1 ctccgggagc tgccgacgga cggagcgccc ccgcccccgc ccggccgccc gcccgccgcc
61 gccatgccct tctccaacag ccacaacgca ctgaagctgc gcttcccggc cgaggacgag
121 ttccccgacc tgagcgccca caacaaccac atggccaagg tgctgacccc cgagctgtac
181 gcggagctgc gcgccaagag cacgccgagc ggcttcacgc tggacgacgt catccagaca
241 ggcgtggaca acccgggcca cccgtacatc atgaccgtgg gctgcgtggc gggcgacgag
301 gagtcctacg aagtgttcaa ggatctcttc gaccccatca tcgaggaccg gcacggcggc
361 tacaagccca gcgatgagca caagaccgac ctcaaccccg acaacctgca gggcggcgac
421 gacctggacc ccaactacgt gctgagctcg cgggtgcgca cgggccgcag catccgtggc
481 ttctgcctcc ccccgcactg cagccgcggg gagcgccgag ccatcgagaa gctcgcggtg
541 gaagccctgt ccagcctgga cggcgacctg gcgggccgat actacgcgct caagagcatg
601 acggaggcgg agcagcagca gctcatcgac gaccacttcc tcttcgacaa gcccgtgtcg
661 cccctgctgc tggcctcggg catggcccgc gactggcccg acgcccgcgg tatctggcac
721 aatgacaata agaccttcct ggtgtgggtc aacgaggagg accacctgcg ggtcatctcc
781 atgcagaagg ggggcaacat gaaggaggtg ttcacccgct tctgcaccgg cctcacccag
841 attgaaactc tcttcaagtc taaggactat gagttcatgt ggaaccctca cctgggctac
901 atcctcacct gcccatccaa cctgggcacc gggctgcggg caggtgtgca tatcaagctg
961 cccaacctgg gcaagcatga gaagttctcg gaggtgctta agcggctgcg acttcagaag
1021 cgaggcacag gcggtgtgga cacggctgcg gtgggcgggg tcttcgacgt ctccaacgct
1081 gaccgcctgg gcttctcaga ggtggagctg gtgcagatgg tggtggacgg agtgaagctg
1141 ctcatcgaga tggaacagcg gctggagcag ggccaggcca tcgacgacct catgcctgcc
1201 cagaaatgaa gcccggccca cacccgacac cagccctgct gcttcctaac ttattgcctg
1261 ggcagtgccc accatgcacc cctgatgttc gccgtctggc gagcccttag ccttgctgta
1321 gagacttccg tcacccttgg tagagtttat ttttttgatg gctaagatac tgctgatgct
1381 gaaataaact agggttttgg cctgaaaaaa aaaaaaaaaa aaaa
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