LOCUS BC008075 2021 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens pleckstrin homology domain containing, family B
(evectins) member 1, mRNA (cDNA clone MGC:2933 IMAGE:3050369),
complete cds.
ACCESSION BC008075
VERSION BC008075.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2021)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2021)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (21-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC008075.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 3 Row: j Column: 24.
FEATURES Location/Qualifiers
source 1..2021
/db_xref="H-InvDB:HIT000033632"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2933 IMAGE:3050369"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2021
/gene="PLEKHB1"
/gene_synonym="KPL1"
/gene_synonym="PHR1"
/gene_synonym="PHRET1"
/db_xref="GeneID:58473"
/db_xref="MIM:607651"
CDS 168..737
/gene="PLEKHB1"
/gene_synonym="KPL1"
/gene_synonym="PHR1"
/gene_synonym="PHRET1"
/codon_start=1
/product="PLEKHB1 protein"
/protein_id="AAH08075.1"
/db_xref="GeneID:58473"
/db_xref="MIM:607651"
/translation="MALVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEE
DRVLIHFNVRDIKIGPECHDVQPPEGRSRDGLLTVNLREGGRLHLCAETKDDALAWKT
ALLEANSTPVRVYSPYQDYYEVVPPNAHEATYVRSYYGPPYAGPGVTHVIVREDPCYS
AGAPLAMGMLAGAATGAALGSLMWSPCWF"
misc_feature 174..488
/gene="PLEKHB1"
/gene_synonym="KPL1"
/gene_synonym="PHR1"
/gene_synonym="PHRET1"
/note="PH; Region: Pleckstrin homology domain. Domain
commonly found in eukaryotic signalling proteins. The
domain family possesses multiple functions including the
abilities to bind inositol phosphates, and various
proteins. PH domains have been found to possess inserted
domains (such as in PLC gamma, syntrophins) and to be
inserted within other domains. Mutations in Brutons
tyrosine kinase (Btk) within its PH domain cause X-linked
agammaglobulinaemia (XLA) in patients. Point mutations
cluster into the positively charged end of the molecule
around the predicted binding site for phosphatidylinositol
lipids"
/db_xref="CDD:smart00233"
BASE COUNT 503 a 575 c 520 g 423 t
ORIGIN
1 gcgctgccgg aggagagaaa tgccccaggc tctccggggc acacaaagcg caggcgcagc
61 gggttgggtg gcagcagcat cgagtagcgg ccgcttaggc agcaacatcc gcaacaagtg
121 tagacaaggt cccgcctgac tccgctctgg aaagtccttt tgaagaaatg gccctggtga
181 ggggcggctg gctgtggaga cagagctcca tcctccgccg ctggaagcgg aactggtttg
241 ccctgtggct ggacgggacc ctgggatact accacgatga gacagcgcag gacgaggagg
301 accgtgtgct catccacttc aatgtccgtg acataaagat cggcccagag tgccatgatg
361 tgcagccccc agagggccgg agccgagatg gcctgctgac tgtgaaccta cgggaaggcg
421 gccgcctgca cctctgtgcg gagaccaagg atgatgccct agcatggaag acagcactgc
481 tggaggcaaa ctccaccccg gtgcgcgtct acagcccgta ccaagactac tacgaggtgg
541 tgccccccaa tgcacacgag gccacgtatg tccgcagcta ctacggaccg ccctacgcag
601 gccctggcgt gacgcacgtg atagtgcggg aggatccctg ctacagcgcc ggcgcccctc
661 tggccatggg catgcttgcg ggagccgcca ctggggcggc gctgggctcg ctcatgtggt
721 cgccctgctg gttctgagcc ctgggactcg gagcactgac ccctgcgctt ggattgctag
781 actcctcttc ctcctggacc ccatcctcta ccatccaagc cctgtcccac tttggcccta
841 tcctctccat tagctccttc cgggtttgga ccattccccc cactccctac ccttaatccc
901 cacatgggaa gaagctatca tcacaggtac aaacatcgct tgaagtcttc acatctacca
961 ctagacaccc ccaaaatctg ttatagacat ttatggatac atttcctcta aacacaacag
1021 ggcacagcaa atacgacttc atttggcttc gagttcccca ggcgctgtag acacaacatg
1081 aatcgggctc tctgctctct ccttagggag ctcgagtcct ggtggggaga acaggagtaa
1141 acaaggactt gacaaagctg aagagttatc agtcctttga caaggacagg tggggcaggg
1201 agcaagacag gtaggctgga agaacagtta ttggcaagta tgcagagccg tgaacgtcat
1261 ggcatgtcca aggaattaaa tgggagttca tttgggctgg ggtggaggct gggatcagac
1321 cgtggtgggc cttcaagcta aggagcttcc taggtgaaag gggagatgtg agccttctct
1381 ggagggaagt ttcatgattg catctataat gaatatattg cctgttttgt gaatactgac
1441 acatgtccat acctaaaaca ctcctgagtt aagtcccatc cttcccacaa acagcttcct
1501 ggctggtacc catgataaca attgagctga acctggggac ccctggttgg ggaacaggtg
1561 agttctattt gagacttcca gccctagaaa gctgcctccg tccagaaatg cctctcacac
1621 caggagctcg gccctctctt tgtagctgtg actgtcaccc tctcaggctt tgtctcatcc
1681 ttcattctga ataagatggc agtgttctcc tctggggcct gatccacctc tacaccagcc
1741 caggaagccc catctgtgcc tgccctcagg tggtccacca gtctccccct ttggttccct
1801 tccagtctct tccccctttc tatcccaatc accaatagaa atgctaacat ccctgcctgg
1861 tagccagact agcccactaa agctcccctg taaatggggg ctccattagt tctgctgccg
1921 agactaataa agatttggtt ggctctagca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1981 aaaaaaaaaa aaaaaaaaaa aacaaaaaaa aaaaaaaaaa a
//