LOCUS BC008011 2089 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens secreted protein, acidic, cysteine-rich (osteonectin),
mRNA (cDNA clone MGC:15881 IMAGE:3529445), complete cds.
ACCESSION BC008011
VERSION BC008011.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2089)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2089)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 23 Row: g Column: 22.
FEATURES Location/Qualifiers
source 1..2089
/db_xref="H-InvDB:HIT000033588"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15881 IMAGE:3529445"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2089
/gene="SPARC"
/db_xref="GeneID:6678"
/db_xref="HGNC:HGNC:11219"
/db_xref="MIM:182120"
CDS 9..920
/gene="SPARC"
/codon_start=1
/product="secreted protein, acidic, cysteine-rich
(osteonectin)"
/protein_id="AAH08011.1"
/db_xref="GeneID:6678"
/db_xref="HGNC:HGNC:11219"
/db_xref="MIM:182120"
/translation="MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVTEVSVG
ANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSEL
TEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLAR
DFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLD
NDKYIALDEWAGCFGIKQKDIDKDLVI"
BASE COUNT 543 a 515 c 505 g 526 t
ORIGIN
1 ccagcaccat gagggcctgg atcttctttc tcctttgcct ggccgggagg gccttggcag
61 cccctcagca agaagccctg cctgatgaga cagaggtggt ggaagaaact gtggcagagg
121 tgactgaggt atctgtggga gctaatcctg tccaggtgga agtaggagaa tttgatgatg
181 gtgcagagga aaccgaagag gaggtggtgg cggaaaatcc ctgccagaac caccactgca
241 aacacggcaa ggtgtgcgag ctggatgaga acaacacccc catgtgcgtg tgccaggacc
301 ccaccagctg cccagccccc attggcgagt ttgagaaggt gtgcagcaat gacaacaaga
361 ccttcgactc ttcctgccac ttctttgcca caaagtgcac cctggagggc accaagaagg
421 gccacaagct ccacctggac tacatcgggc cttgcaaata catcccccct tgcctggact
481 ctgagctgac cgaattcccc ctgcgcatgc gggactggct caagaacgtc ctggtcaccc
541 tgtatgagag ggatgaggac aacaaccttc tgactgagaa gcagaagctg cgggtgaaga
601 agatccatga gaatgagaag cgcctggagg caggagacca ccccgtggag ctgctggccc
661 gggacttcga gaagaactat aacatgtaca tcttccctgt acactggcag ttcggccagc
721 tggaccagca ccccattgac gggtacctct cccacaccga gctggctcca ctgcgtgctc
781 ccctcatccc catggagcat tgcaccaccc gctttttcga gacctgtgac ctggacaatg
841 acaagtacat cgccctggat gagtgggccg gctgcttcgg catcaagcag aaggatatcg
901 acaaggatct tgtgatctaa atccactcct tccacagtac cggattctgt ctttaaccct
961 ccccttcgtg tttcccccaa tgtttaaaat gtttggatgg tttgttgttc tgcctggaga
1021 caaggtgcta acatagattt aagtgaatac attaacggtg ctaaaaatga aaattctaac
1081 ccaagacatg acattcttag ctgtaactta actattaagg ccttttccac acgcattaat
1141 agtcccattt ttctcttgcc atttgtagct ttgcccattg tcttattggc acatgggtgg
1201 acacggatct gctgggctct gccttaaaca cacattgcag cttcaacttt tctctttagt
1261 gttctgtttg aaactaatac ttaccgagtc agactttgtg ttcatttcat ttcagggtct
1321 tggctgcctg tgggcttccc caggtggcct ggaggtgggc aaagggaagt aacagacaca
1381 cgatgttgtc aaggatggtt ttgggactag aggctcagtg gtgggagaga tccctgcaga
1441 acccaccaac cagaacgtgg tttgcctgag gctgtaactg agagaaagat tctggggctg
1501 tcttatgaaa atatagacat tctcacataa gcccagttca tcaccatttc ctcctttacc
1561 tttcagtgca gtttcttttc acattaggct gttggttcaa acttttggga gcacggactg
1621 tcagttctct gggaagtggt cagcgcatcc tgcagggctt ctcctcctct gtcttttgga
1681 gaaccagggc tcttctcagg ggctctaggg actgccaggc tgtttcagcc aggaaggcca
1741 aaatcaagag tgagatgtag aaagttgtaa aatagaaaaa gtggagttgg tgaatcggtt
1801 gttctttcct cacatttgga tgattgtcat aaggttttta gcatgttcct ccttttcttc
1861 accctcccct ttgttcttct attaatcaag agaaacttca aagttaatgg gatggtcgga
1921 tctcacaggc tgagaactcg ttcacctcca agcatttcat gaaaaagctg cttcttatta
1981 atcatacaaa ctctcaccat gatgtgaaga gtttcacaaa tctttcaaaa taaaaagtaa
2041 tgacttagaa actgcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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