LOCUS BC007640 2332 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:15908
IMAGE:3533876), complete cds.
ACCESSION BC007640
VERSION BC007640.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2332)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2332)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 23 Row: i Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33286421.
FEATURES Location/Qualifiers
source 1..2332
/db_xref="H-InvDB:HIT000033349"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15908 IMAGE:3533876"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2332
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/db_xref="GeneID:5315"
/db_xref="HGNC:HGNC:9021"
/db_xref="MIM:179050"
CDS 89..1684
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/codon_start=1
/product="pyruvate kinase, muscle"
/protein_id="AAH07640.1"
/db_xref="GeneID:5315"
/db_xref="HGNC:HGNC:9021"
/db_xref="MIM:179050"
/translation="MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARN
TGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDP
ILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLD
YKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDL
PAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEG
VRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESM
IKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL
QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPI
IAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDV
VIVLTGWRPGSGFTNTMRVVPVP"
BASE COUNT 542 a 650 c 653 g 487 t
ORIGIN
1 gcacagcagc tgcacgcgcc gtggctccgg atctcttcgt ctttgcagcg tagcccgagt
61 cggtcagcgc cggaggacct cagcagccat gtcgaagccc catagtgaag ccgggactgc
121 cttcattcag acccagcagc tgcacgcagc catggctgac acattcctgg agcacatgtg
181 ccgcctggac attgattcac cacccatcac agcccggaac actggcatca tctgtaccat
241 tggcccagct tcccgatcag tggagacgtt gaaggagatg attaagtctg gaatgaatgt
301 ggctcgtctg aacttctctc atggaactca tgagtaccat gcggagacca tcaagaatgt
361 gcgcacagcc acggaaagct ttgcttctga ccccatcctc taccggcccg ttgctgtggc
421 tctagacact aaaggacctg agatccgaac tgggctcatc aagggcagcg gcactgcaga
481 ggtggagctg aagaagggag ccactctcaa aatcacgctg gataacgcct acatggaaaa
541 gtgtgacgag aacatcctgt ggctggacta caagaacatc tgcaaggtgg tggaagtggg
601 cagcaagatc tacgtggatg atgggcttat ttctctccag gtgaagcaga aaggtgccga
661 cttcctggtg acggaggtgg aaaatggtgg ctccttgggc agcaagaagg gtgtgaacct
721 tcctggggct gctgtggact tgcctgctgt gtcggagaag gacatccagg atctgaagtt
781 tggggtcgag caggatgttg atatggtgtt tgcgtcattc atccgcaagg catctgatgt
841 ccatgaagtt aggaaggtcc tgggagagaa gggaaagaac atcaagatta tcagcaaaat
901 cgagaatcat gagggggttc ggaggtttga tgaaatcctg gaggccagtg atgggatcat
961 ggtggctcgt ggtgatctag gcattgagat tcctgcagag aaggtcttcc ttgctcagaa
1021 gatgatgatt ggacggtgca accgagctgg gaagcctgtc atctgtgcta ctcagatgct
1081 ggagagcatg atcaagaagc cccgccccac tcgggctgaa ggcagtgatg tggccaatgc
1141 agtcctggat ggagccgact gcatcatgct gtctggagaa acagccaaag gggactatcc
1201 tctggaggct gtgcgcatgc agcacctgat tgcccgtgag gcagaggctg ccatctacca
1261 cttgcaatta tttgaggaac tccgccgcct ggcgcccatt accagcgacc ccacagaagc
1321 caccgccgtg ggtgccgtgg aggcctcctt caagtgctgc agtggggcca taatcgtcct
1381 caccaagtct ggcaggtctg ctcaccaggt ggccagatac cgcccacgtg cccccatcat
1441 tgctgtgacc cggaatcccc agacagctcg tcaggcccac ctgtaccgtg gcatcttccc
1501 tgtgctgtgc aaggacccag tccaggaggc ctgggctgag gacgtggacc tccgggtgaa
1561 ctttgccatg aatgttggca aggcccgagg cttcttcaag aagggagatg tggtcattgt
1621 gctgaccgga tggcgccctg gctccggctt caccaacacc atgcgtgttg ttcctgtgcc
1681 gtgatggacc ccagagcccc tcctccagcc cctgtcccac ccccttcccc cagcccatcc
1741 attaggccag caacgcttgt agaactcact ctgggctgta acgtggcact ggtaggttgg
1801 gacaccaggg aagaagatca acgcctcact gaaacatggc tgtgtttgca gcctgctcta
1861 gtgggacagc ccagagcctg gctgcccatc atgtggcccc acccaatcaa gggaagaagg
1921 aggaatgctg gactggaggc ccctggagcc agatggcaag agggtgacag cttcctttcc
1981 tgtgtgtact ctgtccagtt cctttagaaa aaatggatgc ccagaggact cccaaccctg
2041 gcttggggtc aagaaacagc cagcaagagt taggggcctt agggcactgg gctgttgttc
2101 cattgaagcc gactctggcc ctggccctta cttgcttctc tagctctcta ggcctctcca
2161 gtttgcacct gtccccaccc tccactcagc tgtcctgcag caaacactcc accctccacc
2221 ttccattttc ccccactact gcagcacctc caggcctgtt gctatagagc ctacctgtat
2281 gtcaataaac aacagctgaa gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//