LOCUS BC007560 3667 bp mRNA linear HUM 01-SEP-2006
DEFINITION Homo sapiens LIM and SH3 protein 1, mRNA (cDNA clone MGC:15487
IMAGE:2988698), complete cds.
ACCESSION BC007560
VERSION BC007560.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3667)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3667)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 22 Row: d Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..3667
/db_xref="H-InvDB:HIT000033299"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15487 IMAGE:2988698"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3667
/gene="LASP1"
/gene_synonym="Lasp-1"
/gene_synonym="MLN50"
/db_xref="GeneID:3927"
/db_xref="HGNC:HGNC:6513"
/db_xref="MIM:602920"
CDS 124..1095
/gene="LASP1"
/gene_synonym="Lasp-1"
/gene_synonym="MLN50"
/codon_start=1
/product="LASP1 protein"
/protein_id="AAH07560.1"
/db_xref="GeneID:3927"
/db_xref="HGNC:HGNC:6513"
/db_xref="MIM:602920"
/translation="MNPNCARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNY
KGYEKKPYCNAHYPKQSFTMVADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVA
DTPELQRIKKTQDQISNIKYHEEFEKSRMGPSGGEGMEPERRDSQDGSSYRRPLEQQQ
PHHIPTSAPVYQQPQQQPVAQSYGGYKEPAAPVSIQRSAPICLQHIPRHRIRPGRDPS
ILQCLCFLKPATACDSYPSSSFFCQLKPSSATSAGSLLWQASPLIDFLVFSLDGTGMG
LSGGGRGPWGRAGMGDLLACGPHLPLCSLPSHPPAQLLTYPHIPGLG"
BASE COUNT 769 a 1031 c 997 g 870 t
ORIGIN
1 ccagctccag ccgccgtcgc tgctgcctgt gtagttgcag ccgcggccgc ctcccgccag
61 ctcgcctcgg ggaacaggac gcgcgtgagc tcaggcgtcc ccgccccagc ttttctcgga
121 accatgaacc ccaactgcgc ccggtgcggc aagatcgtgt atcccacgga gaaggtgaac
181 tgtctggata agttctggca taaagcatgc ttccattgcg agacctgcaa gatgacactg
241 aacatgaaga actacaaggg ctacgagaag aagccctact gcaacgcaca ctaccccaag
301 cagtccttca ccatggtggc ggacaccccg gaaaaccttc gcctcaagca acagagtgag
361 ctccagagtc aggtgcgcta caaggaggag tttgagaaga acaagggcaa aggtttcagc
421 gtagtggcag acacgcccga gctccagaga atcaagaaga cccaggacca gatcagtaac
481 ataaaatacc atgaggagtt tgagaagagc cgcatgggcc ctagcggggg cgagggcatg
541 gagccagagc gtcgggattc gcaggacggc agcagctacc ggcggcccct ggagcagcag
601 cagcctcacc acatcccgac cagtgccccg gtttaccagc agccccagca gcagccggtg
661 gcccagtcct atggtggcta caaggagcct gcagccccag tctccataca gcgcagcgcc
721 cccatctgtc ttcagcacat tccacggcat cgcatccgtc ctgggcgtga cccgtccatt
781 cttcagtgtc tctgtttttt aaaacctgcg acagcttgtg attcctaccc ctcttccagc
841 ttcttttgcc aactgaagcc ttcttctgcc acttctgcgg gctccctcct ctggcaggct
901 tcccccttga tcgacttctt ggttttctct ctggatggaa cgggcatggg cctctctggg
961 ggagggcgag gcccgtgggg cagggctgga atgggagacc tgttggcctg tgggcctcac
1021 ctgcccctct gttctctccc ctcacatcct cctgcccagc tcctcacata cccacacatt
1081 ccagggctgg ggtgagcctg actgccagga ccccaggtca ggggctccct acattcccca
1141 gagtgggatc cacttcttgg ttcctgggat ggcgatgggg actctgccgc tgtgtagaga
1201 ccagtgggat gggctctacc tctctttctc aaagaggggg ctctgcccac ctggggtctc
1261 tctccctacc tccctcctca ggggcaacaa caggagaatg gggttcctgc tgtggggcga
1321 attcatcccc tccccgcgcg ttccttcgca cactgtgatt ttgccctcct gcccacgcag
1381 acctgcagcg ggcaaagagc tcccgaggaa gcacagcttg ggtcaggttc ttgcctttct
1441 taattttagg gacagctacc ggaaggaggg gaacaaggag ttctcttccg cagccccttt
1501 ccccacgccc acccccagtc tccagggacc cttgcctgcc tcctaggctg gaagccatgg
1561 tcccgaagtg tagggcaagg gtgcctcagg accttttggt cttcagcctc cctcagcccc
1621 caggatctgg gttaggtggc cgctcctccc tgctcctcat gggaagatgt ctcagagcct
1681 tccatgacct cccctcccca gcccaatgcc aagtggactt ggagctgcac aaagtcagca
1741 gggaccacta aatctccaag acctggtgtg cggaggcagg agcatgtatg tctgcaggtg
1801 tctgacacgc aagtgtgtga gtgtgagtgt gagagatggg gcgggggtgt gtctgtaggt
1861 gtctctgggc ctgtgtgtgg gtggggttat gtgagggtat gaagagctgt cttcccctga
1921 gagtttcctc agaacccaca gtgagagggg agggctcctg gggcagagaa gttccttagg
1981 ttttctttgg aatgaaattc ctccttcccc ccatctctga gtagaggaag cccaccaatc
2041 tgccctttgc agtgtgcagg gtggaaggta agaggttggt gtggagttgg ggctgccata
2101 gggtctgcag cctgctgggg ctaagcggtg gaggaaggct ctgtcactcc aggcatatgt
2161 ttccccatct ctgtctgggg ctacagaata gggtggcaga agtgtcaccc tgtgggtgtc
2221 tccctcgggg gctcttcccc tagacctccc cctcacttac aaagagctcc cttgaagcaa
2281 gaaagagctc tctgctcatg ggtggctgtg acaaccctgg cctcacttga ttcatctctg
2341 gttttcttgc caccctctgg gagtccccat cccattttca tcctgagccc aaccaggccc
2401 tgccattggc ctcttgtccc ttggcacact tgtacccaca ggtgaggggc aggacctgaa
2461 ggtattggcc tgttcaacaa tcagtcatca tgggtgtttt tgtcaactgc ttgttaattg
2521 atttggggat gtttgccccg aatgagaggt tgaggaaaag actgtgggtg gggaggccct
2581 gcctgaccca tcccttttcc tttctggccc cagcctaggt ggaggcaagt ggaatatctt
2641 atattgggcg atttgggggc tcggggaggc agagaatctc ttgggagtct tgggtggcgc
2701 tggtgcattc tgtttcctct tgatctcaaa gcacaatgtg gatttgggga ccaaaggtca
2761 gggacacatc cccttagagg acctgagttt gggagagtgg tgagtggaag ggaggagcag
2821 caagaagcag cctgttttca ctcagcttaa ttctccttcc cagataaggc aagccagtca
2881 tggaatcttg ctgcaggccc tccctctact cttcctgtcc taaaaatagg ggccgttttc
2941 ttacacaccc ccagagagag gagggactgt cacactggtg ctgagtgacc gggggctgct
3001 gggcgtctgt tctttaccaa aaccatccat ccctagaaga gcacagagcc ctgaggggct
3061 gggctgggct gggctgagcc cctggtcttc tctacagttc acagaggtct ttcagctcat
3121 ttaatcccag gaaagaggca tcaaagctag aatgtgaata taacttttgt ggaccaatac
3181 taagaataac aagaagccca gtggtgagga aagtgcgttc tcccagcact gcctcctgtt
3241 ttctccctct catgtccctc cagggaaaat gactttattg cttaatttct gcctttcccc
3301 cctcacacat gcacttttgg gccttttttt atagctggaa aaaacaaaat accaccctac
3361 aaacctgtat ttaaaaagaa acagaaatga ccacgtgaaa tttgcctctg tccaaacatt
3421 tcatccgtgt gtatgtgtat gtgtgtgagt gtgtgaagcc gccagttcat ctttttatat
3481 ggggttgttg tctcattttg gtctgttttg gtcccctccc tcgtgggctt gtgctcggga
3541 tcaaaccttt ctggcctgtt atgattctga acatttgact tgaaccacaa gtgaatcttt
3601 ctcctggtga ctcaaataaa agtataattt ttacctgcgg aaaaaaaaaa aaaaaaaaaa
3661 aaaaaaa
//