LOCUS BC007536 2879 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphofructokinase, liver, mRNA (cDNA clone MGC:15386
IMAGE:3347301), complete cds.
ACCESSION BC007536
VERSION BC007536.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2879)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2879)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 22 Row: l Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 50346004.
FEATURES Location/Qualifiers
source 1..2879
/db_xref="H-InvDB:HIT000033284"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15386 IMAGE:3347301"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2879
/gene="PFKL"
/gene_synonym="PFK-B"
/db_xref="GeneID:5211"
/db_xref="HGNC:HGNC:8876"
/db_xref="MIM:171860"
CDS 16..2358
/gene="PFKL"
/gene_synonym="PFK-B"
/codon_start=1
/product="phosphofructokinase, liver"
/protein_id="AAH07536.1"
/db_xref="GeneID:5211"
/db_xref="HGNC:HGNC:8876"
/db_xref="MIM:171860"
/translation="MAAVDLEKLRASGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYV
GAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTIIGSARCKAFTTREGRRAAA
YNLVQHGITNLCVIGGDGSLTGANIFRSEWGSLLEELVAEGKISETTARTYSHLNIAG
LVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALV
SALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISS
SYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPAC
VVTLSGNQSVRLPLMECVQMTKEVQKAMDDKRFDEATQLRGGSFENNWNIYKLLAHQK
PPKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISHGHTVYVVHDGFEGLAKGQVQEV
GWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIHALLVVGGFEAYEGVLQLV
EARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRV
FIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLV
LRNEKCHDYYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKA
MLWLSEKLREVYRKGRVFANAPDSACVIGLKKKAVAFSPVTELKKDTDFEHRMPREQW
WLSLRLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDKGF"
BASE COUNT 554 a 875 c 932 g 518 t
ORIGIN
1 gtttcggccg ccgccatggc cgcggtggac ctggagaagc tgcgggcgtc gggcgcgggc
61 aaggccatcg gcgtcctgac cagcggcggc gacgcgcaag gcatgaacgc tgctgtccgg
121 gctgtgacgc gcatgggcat ttatgtgggt gccaaagtct tcctcatcta cgagggctat
181 gagggcctcg tggagggagg tgagaacatc aagcaggcca actggctgag cgtctccaac
241 atcatccagc tgggcggcac tatcattggc agcgctcgct gcaaggcctt taccaccagg
301 gaggggcgcc gggcagcggc ctacaacctg gtccagcacg gcatcaccaa cctgtgcgtc
361 atcggcgggg atggcagcct tacaggtgcc aacatcttcc gcagcgagtg gggcagcctg
421 ctggaggagc tggtggcgga aggtaagatc tcagagacta cagcccggac ctactcgcac
481 ctgaacatcg cgggcctagt gggctccatc gataacgact tctgcggcac cgacatgacc
541 atcggcacgg actcggccct ccaccgcatc atggaggtca tcgatgccat caccaccact
601 gcccagagcc accagaggac cttcgtgctg gaagtgatgg gccggcactg cgggtacctg
661 gcgctggtat ctgcactggc ctcaggggcc gactggctgt tcatccccga ggctccaccc
721 gaggacggct gggagaactt catgtgtgag aggctgggtg agactcggag ccgtgggtcc
781 cgactgaaca tcatcatcat cgctgagggt gccattgacc gcaacgggaa gcccatctcg
841 tccagctacg tgaaggacct ggtggttcag aggctgggct tcgacacccg tgtaactgtg
901 ctgggccacg tgcagcgggg agggacgccc tctgccttcg accggatcct gagcagcaag
961 atgggcatgg aggcggtgat ggcgctgctg gaagccacgc ctgacacgcc ggcctgcgtg
1021 gtcaccctct cggggaacca gtcagtgcgg ctgcccctca tggagtgcgt gcagatgacc
1081 aaggaagtgc agaaagccat ggatgacaag aggtttgacg aggccaccca gctccgtggt
1141 gggagcttcg agaacaactg gaacatttac aagctcctcg cccaccagaa gccccccaag
1201 gagaagtcta acttctccct ggccatcctg aatgtggggg ccccggcggc tggcatgaat
1261 gcggccgtgc gctcggcggt gcggaccggc atctcccatg gacacacagt atacgtggtg
1321 cacgatggct tcgaaggcct agccaagggt caggtgcaag aagtaggctg gcacgacgtg
1381 gccggctggt tggggcgtgg tggctccatg ctggggacca agaggaccct gcccaagggc
1441 cagctggagt ccattgtgga gaacatccgc atctatggta ttcacgccct gctggtggtc
1501 ggtgggtttg aggcctatga aggggtgctg cagctggtgg aggctcgcgg gcgctacgag
1561 gagctctgca tcgtcatgtg tgtcatccca gccaccatca gcaacaacgt ccctggcacc
1621 gacttcagcc tgggctccga cactgctgta aatgccgcca tggagagctg tgaccgcatc
1681 aaacagtctg cctcggggac caagcgccgt gtgttcatcg tggagaccat ggggggttac
1741 tgtggctacc tggccaccgt gactggcatt gctgtggggg ccgacgccgc ctacgtcttc
1801 gaggaccctt tcaacatcca cgacttaaag gtcaacgtgg agcacatgac ggagaagatg
1861 aagacagaca ttcagagggg cctggtgctg cggaacgaga agtgccatga ctactacacc
1921 acggagttcc tgtacaacct gtactcatca gagggcaagg gcgtcttcga ctgcaggacc
1981 aatgtcctgg gccacctgca gcagggtggc gctccaaccc cctttgaccg gaactatggg
2041 accaagctgg gggtgaaggc catgctgtgg ttgtcggaga agctgcgcga ggtttaccgc
2101 aagggacggg tgttcgccaa tgccccagac tcggcctgcg tgatcggcct gaagaagaag
2161 gcggtggcct tcagccccgt cactgagctc aagaaagaca ctgatttcga gcaccgcatg
2221 ccacgggagc agtggtggct gagcctgcgg ctcatgctga agatgctggc acaataccgc
2281 atcagtatgg ccgcctacgt gtcaggggag ctggagcacg tgacccgccg caccctgagc
2341 atggacaagg gcttctgagg ccagccatgc ccacgcccct ccccagcccc cacccatgcc
2401 agcgcagcgc cagggctcag atggggcctg ggctgttgtg tctggagcct gcaggcaggt
2461 gggggctgcg tccctgctca gcccatcccc tgcctctatc cctggccacc tgccaggcct
2521 ccctccggct ggtgtcttga gaccagcctg ccaggccctc cagcaggagg acagagtgcc
2581 ctggggcatc caccttcctg cccaggggac gtggcgctgt cggtgtttgg aggctgctgc
2641 cccctggctt tggcgcccca tgggccctca gcgtctcccc atgctgggct cactacatgg
2701 gccagccctt gctctacctg gccggtaggc tgctggcgcc taggttgtgt tgagaggggg
2761 atgcccctgg ccctgcctca ctgtgacctg ctcctgccca cgtgcagcac ctgtcacctt
2821 ttctagaaat aaaatcaccc tgactgtggg gtgcatcggt caaaaaaaaa aaaaaaaaa
//