LOCUS BC007220 1850 bp mRNA linear HUM 06-MAR-2007
DEFINITION Homo sapiens cysteinyl-tRNA synthetase 2, mitochondrial (putative),
mRNA (cDNA clone MGC:15044 IMAGE:2822557), complete cds.
ACCESSION BC007220
VERSION BC007220.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1850)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1850)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 22 Row: a Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 13375694.
FEATURES Location/Qualifiers
source 1..1850
/db_xref="H-InvDB:HIT000033078"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15044 IMAGE:2822557"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1850
/gene="CARS2"
/gene_synonym="FLJ12118"
/db_xref="GeneID:79587"
/db_xref="HGNC:HGNC:25695"
CDS 11..1705
/gene="CARS2"
/gene_synonym="FLJ12118"
/codon_start=1
/product="cysteinyl-tRNA synthetase 2, mitochondrial
(putative)"
/protein_id="AAH07220.1"
/db_xref="GeneID:79587"
/db_xref="HGNC:HGNC:25695"
/translation="MLRTTRGPGLGPPLLQAALGLGRAGWHWPAGRAASGGRGRAWLQ
PTGRETGVQVYNSLTGRKEPLIVAHAEAASWYSCGPTVYDHAHLGHACSYVRFDIIRR
ILTKVFGCSIVMVMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPT
VYLRVTENIPQIISFIEGIIARGNAYSTAKGNVYFDLKSRGDKYGKLVGVVPGPVGEP
ADSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQLDIHSGGI
DLAFPHHENEIAQCEVFHQCEQWGNYFLHSGHLHAKGKEEKMSKSLKNYITIKDFLKT
FSPDVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLEDARAYMKGQLACGSVRE
AMLWERLSSTKRAVKAALADDFDTPRVVDAILGLAHHGNGQLRASLKEPEGPRSPAVF
GAIISYFEQFFETVGISLANQQYVSGDGSEATLHGVVDELVRFRQKVRQFALAMPEAT
GDARRQQLLERQPLLEACDTLRRGLTAHGINIKDRSSTTSTWELLDPRTKDQKSAG"
BASE COUNT 444 a 478 c 542 g 386 t
ORIGIN
1 ggccgctgac atgttgagga ctacgcgcgg cccaggcctg ggccccccgc tgctccaggc
61 cgcgctgggc cttgggcggg ctgggtggca ctggcctgcg ggccgggcgg cgagcggggg
121 gcgcgggcgg gcctggctgc agcccacggg ccgggagacg ggtgtgcagg tgtacaacag
181 cctcaccggg aggaaggaac ccctaatcgt ggcgcacgcc gaagccgcct cctggtatag
241 ctgtggacca actgtatatg atcatgcgca ccttggccat gcttgctcat atgttagatt
301 tgatatcatt cgaaggatcc taaccaaggt ttttggatgc agcatagtca tggtgatggg
361 tattacagat gtagatgata aaatcatcaa aagagccaat gagatgaata tttcccccgc
421 ttccctcgcc agtctttatg aggaagactt caagcaggac atggcagccc tgaaggttct
481 cccacccacg gtgtacctga gggtaaccga aaatattcct cagataattt ctttcattga
541 aggaatcatt gctcgtggga acgcttattc aacggcaaaa ggcaatgtct acttcgatct
601 gaagtctaga ggagacaagt atggcaaatt ggtcggcgtg gtccctggtc cagtcggaga
661 gccagcggac tctgacaagc gtcatgccag tgacttcgcc ctgtggaagg cggccaaacc
721 ccaggaggtg ttctgggcct ctccctgggg acccgggagg ccgggctggc acatcgagtg
781 ctctgccatc gctagtatgg tatttggaag tcaactggat atccattcag gtgggataga
841 tttagctttt ccacatcatg agaacgaaat tgcacagtgc gaagtctttc atcagtgcga
901 gcagtgggga aattattttc tgcattctgg gcatttgcac gccaaaggca aagaagaaaa
961 aatgtccaaa tcattaaaga actacattac tattaaggac tttctgaaga ccttttcccc
1021 cgatgtcttc cggttcttct gcctgcggag cagctaccgc tcagccatcg actacagtga
1081 cagcgccatg ctccaagctc agcagctgct cctggggctg ggctctttcc tggaggacgc
1141 acgtgcctac atgaaggggc agctggcctg cggctccgtc agggaagcga tgctgtggga
1201 gaggctctcc agcaccaaga gggccgtgaa ggcggccttg gcagatgatt ttgacacacc
1261 cagggtggtt gatgccatcc tgggccttgc acaccacggg aatggacagc tcagggcgtc
1321 cctgaaggaa cctgaagggc cgagaagtcc tgctgtgttt ggtgccatca tctcttactt
1381 tgaacagttt tttgaaactg ttggaatttc tctggcaaat caacagtacg tttcaggaga
1441 cggcagcgag gctaccttgc atggtgtggt ggacgagctg gtgcggttcc ggcagaaggt
1501 ccggcagttt gcgctggcca tgcccgaggc cacgggggac gcccggcggc agcagctcct
1561 agaaaggcag cccctgctgg aagcatgcga caccctgcgc cggggcctga ctgcccacgg
1621 catcaacatc aaggacagaa gcagtacaac atccacgtgg gaactgctgg atccaaggac
1681 aaaagaccaa aaatcagcgg gctgaggatg gagcacagcc atgaacctgc tcacgacaag
1741 atgcacccat gcttctcagg gtcaaggctt tatgttaaag cttcctgtcg gggctgctag
1801 gtcagcatta aagtaaggca accaaaaaaa aaaaaaaaaa aaaaaaaaaa
//