LOCUS BC006547 1818 bp mRNA linear HUM 16-SEP-2003
DEFINITION Homo sapiens eukaryotic translation elongation factor 2, mRNA (cDNA
clone IMAGE:2963048), partial cds.
ACCESSION BC006547
VERSION BC006547.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1818)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1818)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (24-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC006547.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 2 Row: b Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 25453476.
FEATURES Location/Qualifiers
source 1..1818
/db_xref="H-InvDB:HIT000086689"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:2963048"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..>1818
/gene="EEF2"
/gene_synonym="EEF-2"
/gene_synonym="EF2"
/db_xref="GeneID:1938"
/db_xref="MIM:130610"
CDS 69..>1818
/gene="EEF2"
/gene_synonym="EEF-2"
/gene_synonym="EF2"
/codon_start=1
/product="EEF2 protein"
/protein_id="AAH06547.1"
/db_xref="GeneID:1938"
/db_xref="MIM:130610"
/translation="MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAG
IIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINL
IDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKM
DRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGL
HGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKS
ATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKP
LLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKG
PLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRT
ILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVA
VEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHA
CIPIKKKKKKKKKKKKK"
misc_feature 120..1760
/gene="EEF2"
/gene_synonym="EEF-2"
/gene_synonym="EF2"
/note="FusA; Region: Translation elongation factors
(GTPases) [Translation, ribosomal structure and
biogenesis]"
/db_xref="CDD:COG0480"
BASE COUNT 432 a 527 c 535 g 324 t
ORIGIN
1 cgccgccatc ctcggcgcga ctcgcttctt tcggttctac ctgggagaat ccaccgccat
61 ccgccaccat ggtgaacttc acggtagacc agatccgcgc catcatggac aagaaggcca
121 acatccgcaa catgtctgtc atcgcccacg tggaccatgg caagtccacg ctgacagact
181 ccctggtgtg caaggcgggc atcatcgcct cggcccgggc cggggagaca cgcttcactg
241 atacccggaa ggacgagcag gagcgttgca tcaccatcaa gtcaactgcc atctccctct
301 tctacgagct ctcggagaat gacttgaact tcatcaagca gagcaaggac ggtgccggct
361 tcctcatcaa cctcattgac tcccccgggc acgtcgactt ctcctcggag gtgactgctg
421 ccctccgagt caccgatggc gcattggtgg tggtggactg cgtgtcaggc gtgtgcgtgc
481 agacggagac agtgctgcgg caggccattg ccgagcgtat caagcctgtg ctgatgatga
541 acaagatgga ccgcgccctg ctggagctgc agctggagcc cgaggagctc taccagactt
601 tccagcgcat cgtggagaac gtgaacgtca tcatctccac ctacggcgag ggcgagagcg
661 gccccatggg caacatcatg atcgatcctg tcctcggtac cgtgggcttt gggtctggcc
721 tccacgggtg ggccttcacc ctgaagcagt ttgccgagat gtatgtggcc aagttcgccg
781 ccaaggggga gggccagttg gggcctgccg agcgggccaa gaaagtagag gacatgatga
841 agaagctgtg gggtgacagg tactttgacc cagccaacgg caagttcagc aagtcagcca
901 ccagccccga agggaagaag ctgccacgca ccttctgcca gctgatcctg gaccccatct
961 tcaaggtgtt tgatgcgatc atgaatttca agaaagagga gacagcaaaa ctgatagaga
1021 aactggacat caaactggac agcgaggaca aggacaaaga aggcaaaccc ctgctgaagg
1081 ctgtgatgcg ccgctggctg cctgccggag acgccttgtt gcagatgatc accatccacc
1141 tgccctcccc tgtgacggcc cagaagtacc gctgcgagct cctgtacgag gggcccccgg
1201 acgacgaggc tgccatgggc attaaaagct gtgaccccaa aggccctctt atgatgtata
1261 tttccaaaat ggtgccaacc tccgacaaag gtcggttcta cgcctttgga cgagtcttct
1321 cggggctggt ctccactggc ctgaaggtca ggatcatggg gcccaactat acccctggga
1381 agaaggagga cctctacctg aagccaatcc agagaacaat cttgatgatg ggccgctacg
1441 tggagcccat cgaggatgtg ccttgtggga acattgtggg cctcgtgggc gtggaccagt
1501 tcctggtgaa gacgggcacc atcaccacct tcgagcacgc gcacaacatg cgggtgatga
1561 agttcagcgt cagccctgtt gtcagagtgg ccgtggaggc caagaacccg gctgacctgc
1621 ccaagctggt ggaggggctg aagcggctgg ccaagtccga ccccatggtg cagtgcatca
1681 tcgaggagtc gggagagcac atcatcgcgg gcgccggcga gctgcacctg gagatctgcc
1741 tgaaggacct ggaggaggac cacgcctgca tccccatcaa aaaaaaaaaa aaaaaaaaaa
1801 aaaaaaaaaa aaaaaaaa
//