LOCUS BC006509 1458 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens eukaryotic translation elongation factor 1 gamma, mRNA
(cDNA clone MGC:2540 IMAGE:2961341), complete cds.
ACCESSION BC006509
VERSION BC006509.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1458)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1458)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (17-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Oct 8, 2003 this sequence version replaced BC006509.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 2 Row: b Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 25453475.
FEATURES Location/Qualifiers
source 1..1458
/db_xref="H-InvDB:HIT000032886"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2540 IMAGE:2961341"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1458
/gene="EEF1G"
/gene_synonym="EF1G"
/gene_synonym="GIG35"
/db_xref="GeneID:1937"
/db_xref="HGNC:HGNC:3213"
/db_xref="MIM:130593"
CDS 2..1315
/gene="EEF1G"
/gene_synonym="EF1G"
/gene_synonym="GIG35"
/codon_start=1
/product="eukaryotic translation elongation factor 1
gamma"
/protein_id="AAH06509.1"
/db_xref="GeneID:1937"
/db_xref="HGNC:HGNC:3213"
/db_xref="MIM:130593"
/translation="MAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTN
RTPEFLRKFPAGKVPAFEGDDGFCVFESNAIAYYVSNEELRGSTPEAAAQVVQWVSFA
DSDIVPPASTWVFPTLGIMHHNKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLA
DITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDA
KKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKA
KDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQ
TFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQV
DYESYTWRKLDPGSEETQTLVREYFSWEGAFQHVGKAFNQGKIFK"
BASE COUNT 396 a 367 c 389 g 306 t
ORIGIN
1 catggcggct gggaccctgt acacgtatcc tgaaaactgg agggccttca aggctctcat
61 cgctgctcag tacagcgggg ctcaggtccg cgtgctctcc gcaccacccc acttccattt
121 tggccaaacc aaccgcaccc ctgaatttct ccgcaaattt cctgccggca aggtcccagc
181 atttgagggt gatgatggat tctgtgtgtt tgagagcaac gccattgcct actatgtgag
241 caatgaggag ctgcggggaa gtactccaga ggcagcagcc caggtggtgc agtgggtgag
301 ctttgctgat tccgatatag tgcccccagc cagtacctgg gtgttcccca ccttgggcat
361 catgcaccac aacaaacagg ccactgagaa tgcaaaggag gaagtgaggc gaattctggg
421 gctgctggat gcttacttga agacgaggac ttttctggtg ggcgaacgag tgacattggc
481 tgacatcaca gttgtctgca ccctgttgtg gctctataag caggttctag agccttcttt
541 ccgccaggcc tttcccaata ccaaccgctg gttcctcacc tgcattaacc agccccagtt
601 ccgggctgtc ttgggcgaag tgaaactgtg tgagaagatg gcccagtttg atgctaaaaa
661 gtttgcagag acccaaccta aaaaggacac accacggaaa gagaagggtt cacgggaaga
721 gaagcagaag ccccaggctg agcggaagga ggagaaaaag gcggctgccc ctgctcctga
781 ggaggagatg gatgaatgtg agcaggcgct ggctgctgag cccaaggcca aggacccctt
841 cgctcacctg cccaagagta cctttgtgtt ggatgaattt aagcgcaagt actccaatga
901 ggacacactc tctgtggcac tgccatattt ctgggagcac tttgataagg acggctggtc
961 cctgtggtac tcagagtatc gcttccctga agaactcact cagaccttca tgagctgcaa
1021 tctcatcact ggaatgttcc agcgactgga caagctgagg aagaatgcct tcgccagtgt
1081 catccttttt ggaaccaaca atagcagctc catttctgga gtctgggtct tccgaggcca
1141 ggagcttgcc tttccgctga gtccagattg gcaggtggac tacgagtcat acacatggcg
1201 gaaactggat cctggcagcg aggagaccca gacgctggtt cgagagtact tttcctggga
1261 gggggccttc cagcatgtgg gcaaagcctt caatcagggc aagatcttca agtgaacatc
1321 tcttgccatc acctagctgc ctgcacctgc ccttcaggga gatgggggtc attaaaggaa
1381 actgaacatt gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1441 aaaaaaaaaa aaaaaaaa
//