LOCUS BC006485 918 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens vacuolar protein sorting 28 homolog (S. cerevisiae),
mRNA (cDNA clone MGC:695 IMAGE:3535787), complete cds.
ACCESSION BC006485
VERSION BC006485.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 918)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 918)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 8 Row: h Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34452692.
FEATURES Location/Qualifiers
source 1..918
/db_xref="H-InvDB:HIT000032867"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:695 IMAGE:3535787"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..918
/gene="VPS28"
/gene_synonym="MGC60323"
/db_xref="GeneID:51160"
/db_xref="HGNC:HGNC:18178"
CDS 46..711
/gene="VPS28"
/gene_synonym="MGC60323"
/codon_start=1
/product="vacuolar protein sorting 28 homolog (S.
cerevisiae)"
/protein_id="AAH06485.1"
/db_xref="GeneID:51160"
/db_xref="HGNC:HGNC:18178"
/translation="MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFA
VVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLD
CPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLREL
METMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRF
LHA"
BASE COUNT 209 a 284 c 262 g 163 t
ORIGIN
1 cctcgtgccg ggtctcagtg ctgtgccccc cccagagcct agaggatgtt tcatgggatc
61 ccagccacgc cgggcatagg agcccctggg aacaagccgg agctgtatga ggaagtgaag
121 ttgtacaaga acgcccggga gagggagaag tacgacaaca tggcagagct gtttgcggtg
181 gtgaagacaa tgcaagccct ggagaaggcc tacatcaagg actgtgtctc ccccagcgag
241 tacactgcag cctgctcccg gctcctggtc caatacaaag ctgccttcag gcaggtccag
301 ggctcagaaa tcagctctat tgacgaattc tgccgcaagt tccgcctgga ctgcccgctg
361 gccatggagc ggatcaagga ggaccggccc atcaccatca aggacgacaa gggcaacctc
421 aaccgctgca tcgcagacgt ggtctcgctc ttcatcacgg tcatggacaa gctgcgcctg
481 gagatccgcg ccatggatga gatccagccc gacctgcgag agctgatgga gaccatgcac
541 cgcatgagcc acctcccacc cgactttgag ggccgccaga cggtcagcca gtggctgcag
601 accctgagcg gcatgtcggc gtcagatgag ctggacgact cacaggtgcg tcagatgctg
661 ttcgacctgg agtcagccta caacgccttc aaccgcttcc tgcatgcctg agcccggggc
721 actagccctt gcacagaagg gcagagtctg aggcgatggc tcctggtccc ctgtccgcca
781 cacaggccgt ggtcatccac acaactcact gtctgcagct gcctgtctgg tgtctgtctt
841 tggtgtcaga actttggggg ccgggcccct ccccacaata aagatgctct ccgaccttca
901 aaaaaaaaaa aaaaaaaa
//