LOCUS BC006357 3304 bp mRNA linear HUM 08-OCT-2003
DEFINITION Homo sapiens proprotein convertase subtilisin/kexin type 7, mRNA
(cDNA clone MGC:12699 IMAGE:4125594), complete cds.
ACCESSION BC006357
VERSION BC006357.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3304)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3304)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (09-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC006357.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 17 Row: g Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 20336247.
FEATURES Location/Qualifiers
source 1..3304
/db_xref="H-InvDB:HIT000050468"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:12699 IMAGE:4125594"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3304
/gene="PCSK7"
/gene_synonym="LPC"
/gene_synonym="PC7"
/gene_synonym="PC8"
/gene_synonym="SPC7"
/db_xref="GeneID:9159"
/db_xref="MIM:604872"
CDS 150..1925
/gene="PCSK7"
/gene_synonym="LPC"
/gene_synonym="PC7"
/gene_synonym="PC8"
/gene_synonym="SPC7"
/codon_start=1
/product="PCSK7 protein"
/protein_id="AAH06357.1"
/db_xref="GeneID:9159"
/db_xref="MIM:604872"
/translation="MPKGRQKVPHLDAPLGLPTCLWLELAGLFLLVPWVMGLAGTGGP
DGQGTGGPSWAVHLESLEGDGEEETLEQQADALAQAAGLVNAGRIGELQGHYLFVQPA
GHRPALEVEAIRQQVEAVLAGHEAVRWHSEQRLLRRAKRSVHFNDPKYPQQWHLNNRR
SPGRDINVTGVWERNVTGRGVTVVVVDDGVEHTIQDIAPNYSPEGSYDLNSNDPDPMP
HPDVENGNHHGTRCAGEIAAVPNNSFCAVGVAYGSRIAGIRVLDGPLTDSMEAVAFNK
HYQINDIYSCSWGPDDDGKTVDGPHQLGKAALQHGVIAGRQGFGSIFVVASGNGGQHN
DNCNYDGYANSIYTVTIGAVDEEGRMPFYAEECASMLAVTFSGGDKMLRSIVTTDWDL
QKGTGCTEGHTGTSAAAPLAAGMIALMLQVRPCLTWRDVQHIIVFTATRYEDRRAEWV
TNEAGFSHSHQHGFGLLNAWRLVNAAKIWTSVPYLASYVSPVLKENKAIPQSPRSLEV
LWNVSRMDLEMSGLKTLEHVAVTVSITHPRRGSLELKLFCPSGMMSLIGAPRSMDSWL
CVECSRHQGQTKAVRECHEWKIPAR"
misc_feature 633..1559
/gene="PCSK7"
/gene_synonym="LPC"
/gene_synonym="PC7"
/gene_synonym="PC8"
/gene_synonym="SPC7"
/note="Peptidase_S8; Region: Subtilase family. Subtilases
are a family of serine proteases. They appear to have
independently and convergently evolved an Asp/Ser/His
catalytic triad, like that found in the trypsin serine
proteases (see pfam00089). Structure is an alpha/beta fold
containing a 7-stranded parallel beta sheet, order
2314567"
/db_xref="CDD:pfam00082"
BASE COUNT 752 a 923 c 965 g 664 t
ORIGIN
1 cagctgcggc ggggccggtc tgtagccttt gctgagtcaa cactactcac agtgaaagtg
61 atgccttttc tccatttgca caacatgagt gtgacgtggt tagccagacc aggagctcaa
121 cctcatgtag aatccagtcc actgctctga tgccgaaggg gaggcagaaa gtgccacact
181 tggatgcccc cctgggcctg cccacctgcc tctggctgga attagccggg ctcttcttac
241 tggttccctg ggtcatgggc ctggcaggga caggtgggcc tgatggccag ggcacagggg
301 ggccgagctg ggctgtgcac ctggaaagcc tggaaggtga cggggaggaa gagactctgg
361 agcagcaggc ggatgccttg gcccaggcag cagggctggt gaatgctgga cgcatcggag
421 agcttcaggg gcactacctc tttgtccagc ctgctgggca caggccggcc ctggaggtgg
481 aggccatccg gcagcaggtg gaggctgtgt tggctgggca tgaagctgtg cgctggcact
541 cagagcagag gctgctaagg cgggccaagc gcagcgtcca cttcaacgac cccaagtacc
601 cgcagcaatg gcacctgaat aaccgacgga gcccgggcag ggacatcaac gtgacgggtg
661 tgtgggaacg caatgtgact gggcgagggg tgacggtggt ggtagtggat gacggagtgg
721 aacacaccat ccaggacatt gcacccaact atagccctga gggtagctat gacctcaact
781 ctaatgaccc tgaccccatg ccccacccgg atgtggagaa tggcaaccac catggcacgc
841 gatgtgcagg agagatcgcg gctgtgccca acaacagctt ctgtgccgtg ggcgtggcct
901 acgggagccg catcgcaggt atccgggtac tggatggacc tctcacagac agcatggagg
961 cagtggcgtt caacaagcac tatcagatca atgacatcta cagctgcagc tggggaccag
1021 atgacgatgg gaagacagtg gatggccccc atcagcttgg aaaggctgcc ttacaacatg
1081 gggtgattgc tggtcgccag ggctttggga gcatctttgt ggtagccagt ggcaacggag
1141 gccaacacaa cgacaactgc aactacgatg gctacgccaa ctccatctac accgtcacca
1201 taggagctgt ggatgaggag ggacgcatgc ctttctatgc agaagaatgt gcctccatgc
1261 tggcagtcac cttcagtggt ggggacaaga tgcttcggag cattgtgacc actgactggg
1321 accttcagaa gggcactggc tgcactgagg gccacacagg gacctcagct gcagcgcctc
1381 tggcagctgg catgatagcc ttaatgctgc aggtgcggcc ctgcctcacg tggcgtgacg
1441 tccagcacat cattgtcttc acagccaccc ggtatgagga tcgccgtgca gagtgggtca
1501 ccaacgaggc aggcttcagc catagccacc agcacggttt cggcctcctc aacgcctgga
1561 ggctcgtgaa tgcagccaag atctggacat ctgtccctta cttagcatcc tacgtcagtc
1621 ccgtgttaaa agaaaacaag gcgattccgc agtccccccg ttccctggag gtcctgtgga
1681 atgtcagcag gatggacctg gagatgtcag ggctgaagac cctggagcat gtggcagtga
1741 cagtctccat cactcaccca cggcgcggca gcttggagct gaagctgttc tgccccagtg
1801 gcatgatgtc cctcatcggc gccccccgca gcatggactc atggctctgt gtggagtgca
1861 gtagacatca gggacagaca aaggctgtta gagagtgcca tgagtggaaa atacctgcac
1921 gatgacttcg ccctgccctg cccaccgggg ctgaaaattc ctgaggaaga tggttacacc
1981 atcaccccca acaccctcaa gaccctggtg ctggtaggct gtttcaccgt cttctggact
2041 gtttactaca tgctggaagt atatttgagc cagaggaatg tggcttccaa tcaagtttgt
2101 aggagtggac cctgccactg gccccatcgg agccggaaag ccaaggagga agggacagag
2161 ctagaatcag tgccactttg cagcagcaag gatccagacg aagtggaaac agagagcagg
2221 ggccctccca ccacctctga cctccttgcc ccagacctgc tggagcaagg ggactggagc
2281 ctgtcccaga acaagagcgc cctggactgc cctcatcagc acctagacgt accgcacggg
2341 aaggaggagc agatctgctg acctcagggc ctgacagtgt gggacaggct cttctttccc
2401 aaaattaggg agctcttgac agaaagcagt tctgatgctt acatctggaa tctgaggcat
2461 cctctgactc cactcaaaga gggtgagggc cttcttaaga tacaaatggt ggaggattgc
2521 tgccagagaa gtctggtcag agccacaggg tctgcctcca gccaaacggg agcttttggt
2581 gagaaggtgt tggacagggg attggcgccc ccctttggtt tggcctccat cctcatctct
2641 cttgggccaa gccagctgcc taggtccccc aagcatgggg gaccccttcc cacatataag
2701 ttgagaaggt gcctgccata gccaggagcg catctcaatg gaaacatcac tggggtcact
2761 tgggaagagg acttcggggt agaggctggg aggagcccct ggacatgcct gtcctgaaag
2821 cggctgcctc cattatccat tcccaagatg cctgatcaga aaccaaccat gaatgaaccc
2881 ctggctcctt caccaccccc acgattggta tgatgctgcc ggcacagctg ggatacacac
2941 ggctccccca ggcctgagct gcttcactag ggaatcctgc ggcaggactg cagagcagat
3001 ggcagatgca catgttggag gagagagcct tgggagccac tgccactcca gtcctgccac
3061 caccctgtct tcctctgcaa gtgctcaggg aaatggcctt cccgccggag gccagctatc
3121 tgcctgacag gctgtgactc ttctctcaac cttggccttc tcccctcttc tgagctagtt
3181 ggttgaattt tttttaatgc ttaagatttg tttttctctt ttcacagcaa cattttcttg
3241 aatttttttc tgcacagctt ttccaaaata aaaaccttcc aaacaaaaaa aaaaaaaaaa
3301 aaaa
//