LOCUS BC006165 3419 bp mRNA linear HUM 11-SEP-2007
DEFINITION Homo sapiens minichromosome maintenance complex component 2, mRNA
(cDNA clone MGC:13220 IMAGE:3959276), complete cds.
ACCESSION BC006165
VERSION BC006165.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3419)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3419)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 18 Row: n Column: 21.
FEATURES Location/Qualifiers
source 1..3419
/db_xref="H-InvDB:HIT000032643"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:13220 IMAGE:3959276"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3419
/gene="MCM2"
/gene_synonym="BM28"
/gene_synonym="cdc19"
/gene_synonym="D3S3194"
/gene_synonym="MITOTIN"
/db_xref="GeneID:4171"
/db_xref="HGNC:HGNC:6944"
/db_xref="MIM:116945"
CDS 27..2741
/gene="MCM2"
/gene_synonym="BM28"
/gene_synonym="cdc19"
/gene_synonym="D3S3194"
/gene_synonym="MITOTIN"
/codon_start=1
/product="minichromosome maintenance complex component 2"
/protein_id="AAH06165.3"
/db_xref="GeneID:4171"
/db_xref="HGNC:HGNC:6944"
/db_xref="MIM:116945"
/translation="MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGR
DLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDV
EELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATED
GEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKER
ISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDR
ITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNF
VLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPR
SKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNK
VAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPG
GKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVS
REWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARC
TVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGS
HVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYTKERVHPKLNQMDQ
DKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVM
LESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQD
TIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF"
BASE COUNT 760 a 941 c 1013 g 705 t
ORIGIN
1 gtggcggaga ggatcgtggt actgctatgg cggaatcatc ggaatccttc accatggcat
61 ccagcccggc ccagcgtcgg cgaggcaatg atcctctcac ctccagccct ggccgaagct
121 cccggcgtac tgatgccctc acctccagcc ctggccgtga ccttccacca tttgaggatg
181 agtccgaggg gctcctaggc acagaggggc ccctggagga agaagaggat ggagaggagc
241 tcattggaga tggcatggaa agggactacc gcgccatccc agagctggac gcctatgagg
301 ccgagggact ggctctggat gatgaggacg tagaggagct gacggccagt cagagggagg
361 cagcagagcg ggccatgcgg cagcgtgacc gggaggctgg ccggggcctg ggccgcatgc
421 gccgtgggct cctgtatgac agcgatgagg aggacgagga gcgccctgcc cgcaagcgcc
481 gccaggtgga gcgggccacg gaggacggcg aggaggacga ggagatgatc gagagcatcg
541 agaacctgga ggatctcaaa ggccactctg tgcgcgagtg ggtgagcatg gcgggccccc
601 ggctggagat ccaccaccgc ttcaagaact tcctgcgcac tcacgtcgac agccacggcc
661 acaacgtctt caaggagcgc atcagcgaca tgtgcaaaga gaaccgtgag agcctggtgg
721 tgaactatga ggacttggca gccagggagc acgtgctggc ctacttcctg cctgaggcac
781 cggcggagct gctgcagatc tttgatgagg ctgccctgga ggtggtactg gccatgtacc
841 ccaagtacga ccgcatcacc aaccacatcc atgtccgcat ctcccacctg cctctggtgg
901 aggagctgcg ctcgctgagg cagctgcatc tgaaccagct gatccgcacc agtggggtgg
961 tgaccagctg cactggcgtc ctgccccagc tcagcatggt caagtacaac tgcaacaagt
1021 gcaatttcgt cctgggtcct ttctgccagt cccagaacca ggaggtgaaa ccaggctcct
1081 gtcctgagtg ccagtcggcc ggcccctttg aggtcaacat ggaggagacc atctatcaga
1141 actaccagcg tatccgaatc caggagagtc caggcaaagt ggcggctggc cggctgcccc
1201 gctccaagga cgccattctc ctcgcagatc tggtggacag ctgcaagcca ggagacgaga
1261 tagagctgac tggcatctat cacaacaact atgatggctc cctcaacact gccaatggct
1321 tccctgtctt tgccactgtc atcctagcca accacgtggc caagaaggac aacaaggttg
1381 ctgtagggga actgaccgat gaagatgtga agatgatcac tagcctctcc aaggatcagc
1441 agatcggaga gaagatcttt gccagcattg ctccttccat ctatggtcat gaagacatca
1501 agagaggcct ggctctggcc ctgttcggag gggagcccaa aaacccaggt ggcaagcaca
1561 aggtacgtgg tgatatcaac gtgctcttgt gcggagaccc tggcacagcg aagtcgcagt
1621 ttctcaagta tattgagaaa gtgtccagcc gagccatctt caccactggc cagggggcgt
1681 cggctgtggg cctcacggcg tatgtccagc ggcaccctgt cagcagggag tggaccttgg
1741 aggctggggc cctggttctg gctgaccgag gagtgtgtct cattgatgaa tttgacaaga
1801 tgaatgacca ggacagaacc agcatccatg aggccatgga gcaacagagc atctccatct
1861 cgaaggctgg catcgtcacc tccctgcagg ctcgctgcac ggtcattgct gccgccaacc
1921 ccataggagg gcgctacgac ccctcgctga ctttctctga gaacgtggac ctcacagagc
1981 ccatcatctc acgctttgac atcctgtgtg tggtgaggga caccgtggac ccagtccagg
2041 acgagatgct ggcccgcttc gtggtgggca gccacgtcag acaccacccc agcaacaagg
2101 aggaggaggg gctggccaat ggcagcgctg ctgagcccgc catgcccaac acgtatggcg
2161 tggagcccct gccccaggag gtcctgaaga agtacatcat ctacaccaag gagagggtcc
2221 acccgaagct caaccagatg gaccaggaca aggtggccaa gatgtacagt gacctgagga
2281 aagaatctat ggcgacaggc agcatcccca ttacggtgcg gcacatcgag tccatgatcc
2341 gcatggcgga ggcccacgcg cgcatccatc tgcgggacta tgtgatcgaa gacgacgtca
2401 acatggccat ccgcgtgatg ctggagagct tcatagacac acagaagttc agcgtcatgc
2461 gcagcatgcg caagactttt gcccgctacc tttcattccg gcgtgacaac aatgagctgt
2521 tgctcttcat actgaagcag ttagtggcag agcaggtgac atatcagcgc aaccgctttg
2581 gggcccagca ggacactatt gaggtccctg agaaggactt ggtggataag gctcgtcaga
2641 tcaacatcca caacctctct gcattttatg acagtgagct cttcaggatg aacaagttca
2701 gccacgacct gaaaaggaaa atgatcctgc agcagttctg aggccctatg ccatccataa
2761 ggattccttg ggattctggt ttggggtggt cagtgccctc tgtgctttat ggacacaaaa
2821 ccagagcact tgatgaactc ggggtactag ggtcagggct tatagcagga tgtctggctg
2881 cacctggcat gactgtttgt ttctccaagc ctgctttgtg cttctcacct ttgggtggga
2941 tgccttgcca gtgtgtctta cttggttgct gaacatcttg ccacctccga gtgctttgtc
3001 tccactcagt accttggatc agagctgctg agttcaggat gcctgcgtgt ggtttaggtg
3061 ttagccttct tacatggatg tcaggagagc tgctgccctc ttggcgtgag ttgcgtattc
3121 aggctgcttt tgctgccttt ggccagagag ctggttgaag atgtttgtaa tcgttttcag
3181 tctcctgcag gtttctgtgc ccctgtggtg gaagagggca cgacagtgcc agcgcagcgt
3241 tctgggctcc tcagtcgcag gggtgggatg tgagtcatgc ggattatcca ctcgccacag
3301 ttatcagctg ccattgctcc ctgtctgttt ccccactctc ttatttgtgc attcggtttg
3361 gtttctgtag ttttaatttt taataaagtt gaataaaata taaaaaaaaa aaaaaaaaa
//