LOCUS BC006114 2046 bp mRNA linear HUM 06-JUN-2006
DEFINITION Homo sapiens cholinergic receptor, nicotinic, alpha 3, mRNA (cDNA
clone MGC:12991 IMAGE:3503197), complete cds.
ACCESSION BC006114
VERSION BC006114.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2046)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2046)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 18 Row: d Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19923121.
FEATURES Location/Qualifiers
source 1..2046
/db_xref="H-InvDB:HIT000032614"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:12991 IMAGE:3503197"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2046
/gene="CHRNA3"
/db_xref="GeneID:1136"
/db_xref="HGNC:HGNC:1957"
/db_xref="MIM:118503"
CDS 502..1971
/gene="CHRNA3"
/codon_start=1
/product="CHRNA3 protein"
/protein_id="AAH06114.1"
/db_xref="GeneID:1136"
/db_xref="HGNC:HGNC:1957"
/db_xref="MIM:118503"
/translation="MGSGPLSLPLALSPPRLLLLLLLSLLPVARASEAEHRLFERLFE
DYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSD
YGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCK
IDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHD
IKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLC
ISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPT
THTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSNLNCFSRAESKGCK
EGYPCQDGMCGYCHHRRIKISNFSANLTRSSSSESVDAVLSLSALSPEIKEAIQSVKY
IAENMKAQNEAKEEQKAQEIQQLKRKEKSTETSDQEPGL"
BASE COUNT 469 a 639 c 516 g 422 t
ORIGIN
1 gtccttccca gggtgtgtta gcgtctctcc tacggcgccc gcctagcccg gtggtcccaa
61 ccccctgcgg gagctgcaca cgccccggaa acctgggaca gaaactgagt ccctctcctt
121 cctggtggtg gtgacagcac ctgctcagat ctggtcggac gccgccggcc ggagcaccca
181 gcccggcgga gaaggagctc gcccggcgct ggggactggg acctggagcc ccttccccta
241 ccgcacgtac gccccgcccc gcgcacgccc gcccgcccgc ccgcgcctgg cgcagcttca
301 ctccggatgg ttcctgtcct cccgcgggtc cgagggcgct ggaaacccag cggcggcgaa
361 gcggagagga gccccgcgcg tctccgcccg cacggctcca ggtctggggt ctgcgctgga
421 gccgcgcggg gagaggccgt ctctgcgacc gccgcgcccg ctcccgaccg tccgggtccg
481 cggccagccc ggccaccagc catgggctct ggcccgctct cgctgcccct ggcgctgtcg
541 ccgccgcggc tgctgctgct gctgctgctg tctctgctgc cagtggccag ggcctcagag
601 gctgagcacc gtctatttga gcggctgttt gaagattaca atgagatcat ccggcctgta
661 gccaacgtgt ctgacccagt catcatccat ttcgaggtgt ccatgtctca gctggtgaag
721 gtggatgaag taaaccagat catggagacc aacctgtggc tcaagcaaat ctggaatgac
781 tacaagctga aatggaaccc ctctgactat ggtggggcag agttcatgcg tgtccctgca
841 cagaagatct ggaagccaga cattgtgctg tataacaatg ctgttgggga tttccaggtg
901 gacgacaaga ccaaagcctt actcaagtac actggggagg tgacttggat acctccggcc
961 atctttaaga gctcctgtaa aatcgacgtg acctacttcc cgtttgatta ccaaaactgt
1021 accatgaagt tcggttcctg gtcctacgat aaggcgaaaa tcgatctggt cctgatcggc
1081 tcttccatga acctcaagga ctattgggag agcggcgagt gggccatcat caaagcccca
1141 ggctacaaac acgacatcaa gtacaactgc tgcgaggaga tctaccccga catcacatac
1201 tcgctgtaca tccggcgcct gcccttgttc tacaccatca acctcatcat cccctgcctg
1261 ctcatctcct tcctcactgt gctcgtcttc tacctgccct ccgactgcgg tgagaaggtg
1321 accctgtgca tttctgtcct cctctccctg acggtgtttc tcctggtgat cactgagacc
1381 atcccttcca cctcgctggt catccccctg attggagagt acctcctgtt caccatgatt
1441 tttgtaacct tgtccatcgt catcaccgtc ttcgtgctca acgtgcacta cagaaccccg
1501 acgacacaca caatgccctc atgggtgaag actgtattct tgaacctgct ccccagggtc
1561 atgttcatga ccaggccaac aagcaacgag ggcaacgctc agaagccgag gcccctctac
1621 ggtgccgagc tctcaaatct gaattgcttc agccgcgcag agtccaaagg ctgcaaggag
1681 ggctacccct gccaggacgg gatgtgtggt tactgccacc accgcaggat aaaaatctcc
1741 aatttcagtg ctaacctcac gagaagctct agttctgaat ctgttgatgc tgtgctgtcc
1801 ctctctgctt tgtcaccaga aatcaaagaa gccatccaaa gtgtcaagta tattgctgaa
1861 aatatgaaag cacaaaatga agccaaagag gaacaaaaag cccaagagat ccaacaattg
1921 aaacgaaaag aaaagtccac agaaacatcc gatcaagaac ctgggctatg aatttccaat
1981 cttcaacaac ctgttaggaa aaaaataaaa atgcattaaa taattgtaaa aaaaaaaaaa
2041 aaaaaa
//