LOCUS BC005811 2251 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate dehydrogenase kinase, isozyme 2, mRNA (cDNA
clone MGC:10386 IMAGE:3831465), complete cds.
ACCESSION BC005811
VERSION BC005811.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2251)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2251)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC005811.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 14 Row: n Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31543388.
FEATURES Location/Qualifiers
source 1..2251
/db_xref="H-InvDB:HIT000032454"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:10386 IMAGE:3831465"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2251
/gene="PDK2"
/db_xref="GeneID:5164"
/db_xref="HGNC:HGNC:8810"
/db_xref="MIM:602525"
CDS 50..1273
/gene="PDK2"
/codon_start=1
/product="pyruvate dehydrogenase kinase, isozyme 2"
/protein_id="AAH05811.1"
/db_xref="GeneID:5164"
/db_xref="HGNC:HGNC:8810"
/db_xref="MIM:602525"
/translation="MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNA
CEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKD
PEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYL
SRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMA
SPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVM
VALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISR
LYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWC
VPSTEPKNTSTYRVS"
BASE COUNT 468 a 737 c 591 g 455 t
ORIGIN
1 gagcgctgcc cgcgcgggga ccacaaccca agtcgcggcc gccgcagcca tgcgctgggt
61 gtgggcgctg ctgaagaatg cgtccctggc aggggcgccc aagtacatag agcacttcag
121 caagttctcc ccgtccccgc tgtccatgaa gcagtttctg gacttcggat ccagcaatgc
181 ctgtgagaaa acctccttca ccttcctcag gcaggagctg cctgtgcgcc tggccaacat
241 catgaaagag atcaacctgc ttcccgaccg agtgctgagc acaccctccg tgcagctggt
301 gcagagctgg tatgtccaga gcctcctgga catcatggag ttcctggaca aggatcccga
361 ggaccatcgc accctgagcc agttcactga cgccctggtc accatccgga accggcacaa
421 cgacgtggtg cccaccatgg cacaaggcgt gcttgagtac aaggacacct acggcgatga
481 ccccgtctcc aaccagaaca tccagtactt cctggaccgc ttctacctca gccgcatctc
541 catccgcatg ctcatcaacc agcacaccct catctttgat ggcagcacca acccagccca
601 tcccaaacac atcggcagca tcgaccccaa ctgcaacgtc tctgaggtgg tcaaagatgc
661 ctacgacatg gctaagctcc tgtgtgacaa gtattacatg gcctcacctg acctggagat
721 ccaggagatc aatgcagcca actccaaaca gccgattcac atggtctacg tcccctccca
781 cctctaccac atgctctttg agctcttcaa gaatgccatg agggcgactg tggaaagcca
841 tgagtccagc ctcattctcc cacccatcaa ggtcatggtg gccttgggtg aggaagatct
901 gtccatcaag atgagtgacc gaggtggggg tgttcccttg aggaagattg agcgactctt
961 cagctacatg tactccacag cacccacccc ccagcctggc accgggggaa cgccgctggc
1021 tggctttggt tatgggctcc ccatttcccg cctctacgcc aagtacttcc agggagacct
1081 gcagctcttc tccatggaag gctttgggac cgatgctgtc atctatctca aggccctgtc
1141 cacggactcg gtggagcgcc tgcctgtcta caacaagtca gcctggcgcc actaccagac
1201 catccaggag gccggcgact ggtgtgtgcc cagcacggag cccaagaaca cgtccacgta
1261 ccgcgtcagc taagggccgc cgtgcatctg cacctgagag gacggactgc cgcctctggg
1321 tccccccacc gtggtgcccc tcaccatcct cctgggggag cagggggtgg gttctccctg
1381 atgaccaggt tctgtctcta tgcaagtcac tgcggtgata ggtctgtgat ggtccctaag
1441 tgccagtcca tctctgtgga gacccctcgg tggcctccct atctctgtgg gcgatgcctg
1501 agggttaggg atgtctccac cctgatgggg tgtcccagag acattttccc atggcagtcc
1561 tcctctctga gaccagggct gtcacttttc tgccaggggt actgggtccc cctcagcacc
1621 ctccacagca caggccttcc aagtggatgt cccgttgcct tattccccca gcccacaaag
1681 gcaccctggc cttggtctgc tgaagtgtta ggaagagggt gggtgccctc cagacctggg
1741 gactgagtgg ggaaaggagt tacacccgtg agtggggaat gaggctggtc ctgcagcctc
1801 tccctccgct cagggcttga aggtcggtgg cggagggggt ggctctcaca gggcccaact
1861 ctaaagtgga agaaccttgt tagaccgaga gcttgccatc cagccaagct gctcgaggcc
1921 ctgcagtggc cttggcaatg tctgtgccac ctcctgagcc ctcccagcat gtcctcacat
1981 gctcatgccc acccgctcct ccacaagcct agtccatcct gcctgagctc cagcccccag
2041 cccccactgt gcccagacat gtgtgctcag ggtggctttc tccctaggac cttctgtgta
2101 tatagttagt tttataaccc tgaatgcccc cacccttccc ctaagcacac aggggttaaa
2161 gctgtgtgtc cctcccagtg gctgtggcag tgacagtgac acccacaccc acagtaaaga
2221 ggagactgaa tgaaaaaaaa aaaaaaaaaa a
//