LOCUS BC005266 1423 bp mRNA linear HUM 11-SEP-2007
DEFINITION Homo sapiens primase, DNA, polypeptide 1 (49kDa), mRNA (cDNA clone
MGC:12308 IMAGE:3686937), complete cds.
ACCESSION BC005266
VERSION BC005266.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1423)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1423)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (27-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 16 Row: m Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41349493.
FEATURES Location/Qualifiers
source 1..1423
/db_xref="H-InvDB:HIT000032334"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:12308 IMAGE:3686937"
/tissue_type="Bone marrow, chronic myelogenous leukemia"
/clone_lib="NIH_MGC_54"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1423
/gene="PRIM1"
/gene_synonym="p49"
/db_xref="GeneID:5557"
/db_xref="HGNC:HGNC:9369"
/db_xref="MIM:176635"
CDS 38..1300
/gene="PRIM1"
/gene_synonym="p49"
/codon_start=1
/product="primase, DNA, polypeptide 1 (49kDa)"
/protein_id="AAH05266.1"
/db_xref="GeneID:5557"
/db_xref="HGNC:HGNC:9369"
/db_xref="MIM:176635"
/translation="METFDPTELPELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHRE
FSFTLKDDIYIRYQSFNNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQ
EKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYS
GRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEKIHPFIRKSINI
IKKYFEEYALVNQDILENKESWDKILALVPETIHDELQQSFQKSHNSLQRWEHLKKVA
SRYQNNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPI
DLQKVDQFDPFTVPTISFICRELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYV
KVFEHFLENLDKSRKGELLKKSDLQKDF"
BASE COUNT 486 a 269 c 290 g 378 t
ORIGIN
1 cctgtggtaa tccttaccgt ggcgagttcc gcgctcaatg gagacgtttg accccaccga
61 gctgcccgag ctgcttaaac tttattaccg gaggctcttt ccctactctc agtactatcg
121 ctggctcaac tacggtggag tgataaagaa ttactttcaa caccgtgaat tttcattcac
181 attgaaagat gatatttaca ttcgctacca atccttcaac aaccagagtg atctggaaaa
241 ggagatgcag aaaatgaatc catacaagat tgatataggc gcagtatatt ctcacagacc
301 caatcaacac aatacagtga agctgggagc tttccaggct caggaaaaag aactggtatt
361 tgacattgac atgacagact atgacgatgt gaggagatgt tgtagttctg cagacatatg
421 tcctaagtgc tggaccctca tgacaatggc catacgcatc attgacagag cattgaagga
481 ggactttgga tttaagcatc gtctctgggt atattctgga aggagaggtg ttcattgttg
541 ggtctgtgat gaatcagtta gaaaactgtc ttctgcagta cgttctggga tagttgagta
601 tttgagcctt gtaaagggtg gtcaagacgt taaaaagaaa gttcacctaa gtgaaaaaat
661 tcaccctttt atcagaaaat ctataaacat aataaaaaaa tactttgaag aatatgcctt
721 ggttaatcaa gatattctcg aaaataaaga aagctgggat aagattttag cccttgttcc
781 tgaaacaatt catgatgaac ttcaacaaag cttccaaaag tctcacaatt cacttcagcg
841 ttgggagcac ttgaagaaag tagccagcag atatcagaat aacatcaaaa atgacaaata
901 tggaccctgg ctggagtggg agattatgct ccagtactgt tttccacggc tggatatcaa
961 tgtcagcaaa ggaatcaatc atctactgaa gagccctttt agtgttcatc ctaaaacagg
1021 tcgcatatct gtgcctattg atttgcagaa agtggaccag tttgatccat ttactgttcc
1081 gaccataagc ttcatctgcc gtgaattgga tgccatttcc actaatgaag aggaaaaaga
1141 ggagaatgaa gctgaatctg atgtcaaaca tagaaccaga gattataaga agaccagtct
1201 agcaccttat gtgaaagttt ttgaacattt tcttgaaaat ctggataaat cccgaaaagg
1261 agaacttctt aagaagagtg atttacaaaa agatttctga agacagagct cctcaaacca
1321 ttgtggatat cttctgcctt caaccacaga tcaaatactt caagagccat ttaataaata
1381 tggcagaact atgaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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