LOCUS BC005164 2174 bp mRNA linear HUM 21-JUL-2008
DEFINITION Homo sapiens apoptosis enhancing nuclease, mRNA (cDNA clone
MGC:4669 IMAGE:3531883), complete cds.
ACCESSION BC005164
VERSION BC005164.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2174)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2174)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: f Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21362093.
FEATURES Location/Qualifiers
source 1..2174
/db_xref="H-InvDB:HIT000032259"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4669 IMAGE:3531883"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2174
/gene="AEN"
/gene_synonym="pp12744"
/db_xref="GeneID:64782"
/db_xref="HGNC:HGNC:25722"
/db_xref="MIM:610177"
CDS 68..1051
/gene="AEN"
/gene_synonym="pp12744"
/codon_start=1
/product="AEN protein"
/protein_id="AAH05164.1"
/db_xref="GeneID:64782"
/db_xref="HGNC:HGNC:25722"
/db_xref="MIM:610177"
/translation="MVPREAPESAQCLCPSLTIPNAKDVLRKRHKRRSRQHQRFMARK
ALLQEQGLLSMPPEPGSSPLPTPFGAATATEAASSGKQCLRAGSGSAPCSRRPAPGKA
SGPLPSKCVAIDCEMVGTGPRGRVSELARCSIVSYHGDVLYDKYIRPEMPIADYRTRW
SGITRQHMRKAVPFQVAQKEILKLLKGKVVVGHALHNDFQALKYVHPRSQTRDTTYVP
NFLSEPGLHTRARVSLKDLALQLLHKKIQVGQHGHSSVEDATTAMELYRLVEVQWEQQ
EARSLWTCPEDREPDSSTDMEQYMEDQSTQYWALKQKSEKQDSGLNSGAFV"
BASE COUNT 490 a 589 c 608 g 487 t
ORIGIN
1 cgatgacctg ccccattgga agattactcc ccaggcttcc cttgccccaa gcagtgagct
61 gactggaatg gtaccccggg aggcccctga gtctgctcag tgcctgtgcc cttccctcac
121 catcccaaat gccaaggatg tgcttcggaa gaggcacaag agaaggagcc gacagcacca
181 gcggttcatg gcccggaagg ccttgctgca ggagcagggg ctgctgagca tgcctccaga
241 gccagggtcc tccccactgc ccaccccttt cggggcagcg acagcaactg aagctgccag
301 cagtgggaag cagtgtctga gggctggatc tggcagtgcc ccatgcagca gaaggcctgc
361 tcccgggaaa gcctcagggc ccttgcccag caagtgtgtg gctatcgact gtgagatggt
421 gggcacggga ccccgagggc gggtaagcga gctggcccgc tgttccattg tgagctacca
481 tggcgatgtc ctctatgaca agtacatcag gcctgagatg cccatcgctg actaccgtac
541 ccgctggagt ggcatcactc ggcagcacat gcgcaaggct gtccccttcc aggtggccca
601 gaaagagatc cttaagctcc tgaagggcaa ggtggtggtg gggcacgcgc tgcacaacga
661 cttccaggcg ctcaagtatg tccaccctcg gagccagacc cgggatacca cctatgtccc
721 aaacttcctc agcgagcccg gcctccacac ccgggcccgg gtctctctaa aggacctggc
781 cctgcagctg ctgcacaaga agatccaggt gggccagcac gggcactcat cagtagaaga
841 tgccacgaca gccatggagc tctaccggct ggtggaggtg cagtgggaac agcaggaggc
901 ccgcagcctc tggacctgcc ccgaggacag agaacctgac agcagcacag acatggaaca
961 gtacatggag gaccagtcca cccagtactg ggctcttaag caaaagtctg agaaacaaga
1021 cagtggtttg aattctgggg cctttgtgta ggattgtgcc tgacctttat ttatttatta
1081 acagcagggc cactcgtcta gggcagtgga gtctgcgtgt ctcctggggc tggggcaggg
1141 cattggcagt tacgcagtgg ccctgaacct ggtctggtgc ccccgaacct ggtctgatgc
1201 cccctcagct ctttgacaat cactgtggct gttgggtttt ctcctatttc taaaaatgtt
1261 ctcttctttc ctaagtgaca gttttgaagt attggataac caagagctca ggtcacacag
1321 accttggagc cccatctttg cttgcagctt agctttgaga tactaagcaa gcgagggact
1381 tcactcttga gcctgttttt tcctcatctg taaagtgggg atagtggtac ggcctacctc
1441 atagggttgt aatgagcact aaatgtgaag tgcttggcac agtgcctggc acatggtgag
1501 ccacagctac tgtgagtttc tgtttatccc cctttttttt ttttaagccc attgtttatt
1561 ctttgagaat ttgttgtaac ttcttcagga taacacctga gtccacaggc tgagcagctg
1621 tggcccagac agaactgctc cggcttggct gttccagcag gtggggcgct ggcctcggtg
1681 agggcacagc agcaaggttc acggatatcc gtgtgtcttg tctgtggcca ccaggcacag
1741 gtttggcttc cggtcagtgt cccgacactg tgcgggaggt gacaacagag caaagcagcg
1801 caggggtcag ggaggtggag acactgctga aatcacacta ccccaccctc agctgaagcc
1861 ccacgttcca caaacttggg gtcatagatt gtccagtcac tggctccctc cctgtcagca
1921 cagcacagag gaaggggcta actgaatctt ttaccacttc tggcctggct ccagaacttt
1981 gttctagatt ccttaaaagt cggtagctga tgtcaaactc aattgagcag tagctttgat
2041 cccttggtct gggggtcgaa ggaagatggc gctgttatca gcggggaaat gtactattta
2101 agatcagctt tgttgtaaaa ccatttgttc tagaataaaa ctcaattgga aacgtgaaaa
2161 aaaaaaaaaa aaaa
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