LOCUS BC004979 1364 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens APEX nuclease (multifunctional DNA repair enzyme) 1,
mRNA (cDNA clone MGC:3855 IMAGE:2905681), complete cds.
ACCESSION BC004979
VERSION BC004979.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1364)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1364)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (21-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: p Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 18375500.
FEATURES Location/Qualifiers
source 1..1364
/db_xref="H-InvDB:HIT000032128"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3855 IMAGE:2905681"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1364
/gene="APEX1"
/gene_synonym="APE"
/gene_synonym="APE1"
/gene_synonym="APEN"
/gene_synonym="APX"
/gene_synonym="HAP1"
/gene_synonym="REF-1"
/gene_synonym="REF1"
/db_xref="GeneID:328"
/db_xref="HGNC:HGNC:587"
/db_xref="MIM:107748"
CDS 130..1086
/gene="APEX1"
/gene_synonym="APE"
/gene_synonym="APE1"
/gene_synonym="APEN"
/gene_synonym="APX"
/gene_synonym="HAP1"
/gene_synonym="REF-1"
/gene_synonym="REF1"
/codon_start=1
/product="APEX nuclease (multifunctional DNA repair
enzyme) 1"
/protein_id="AAH04979.1"
/db_xref="GeneID:328"
/db_xref="HGNC:HGNC:587"
/db_xref="MIM:107748"
/translation="MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPAL
YEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSEN
KLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVA
EFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEI
DLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSK
NVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL"
BASE COUNT 381 a 325 c 343 g 315 t
ORIGIN
1 ccggtgcaga tacggggttg ctcttttgct cataagaggg gcttcgctgg cagtctgaac
61 ggcaacgcgg taaaaatatt gcttcggtgg gtgacgcggt acagctgccc aagggcgttc
121 gtaacgggaa tgccgaagcg tgggaaaaag ggagcggtgg cggaagacgg ggatgagctc
181 aggacagagc cagaggccaa gaagagtaag acggccgcaa agaaaaatga caaagaggca
241 gcaggagagg gcccagccct gtatgaggac cccccagatc agaaaacctc acccagtggc
301 aaacctgcca cactcaagat ctgctcttgg aatgtggatg ggcttcgagc ctggattaag
361 aagaaaggat tagattgggt aaaggaagaa gccccagata tactgtgcct tcaagagacc
421 aaatgttcag agaacaaact accagctgaa cttcaggagc tgcctggact ctctcatcaa
481 tactggtcag ctccttcgga caaggaaggg tacagtggcg tgggcctgct ttcccgccag
541 tgcccactca aagtttctta cggcataggc gatgaggagc atgatcagga aggccgggtg
601 attgtggctg aatttgactc gtttgtgctg gtaacagcat atgtacctaa tgcaggccga
661 ggtctggtac gactggagta ccggcagcgc tgggatgaag cctttcgcaa gttcctgaag
721 ggcctggctt cccgaaagcc ccttgtgctg tgtggagacc tcaatgtggc acatgaagaa
781 attgaccttc gcaaccccaa ggggaacaaa aagaatgctg gcttcacgcc acaagagcgc
841 caaggcttcg gggaattact gcaggctgtg ccactggctg acagctttag gcacctctac
901 cccaacacac cctatgccta caccttttgg acttatatga tgaatgctcg atccaagaat
961 gttggttggc gccttgatta ctttttgttg tcccactctc tgttacctgc attgtgtgac
1021 agcaagatcc gttccaaggc cctcggcagt gatcactgtc ctatcaccct atacctagca
1081 ctgtgacacc acccctaaat cactttgagc ctgggaaata agccccctca actaccattc
1141 cttctttaaa cactcttcag agaaatctgc attctatttc tcatgtataa aactaggaat
1201 cctccaacca ggctcctgtg atagagttct tttaagccca agatttttta tttgagggtt
1261 ttttgttttt taaaaaaaaa ttgaacaaag actactaatg actttgtttg aattatccac
1321 atgaaaataa agagccatag tttcaaaaaa aaaaaaaaaa aaaa
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