LOCUS BC004974 2114 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens secreted protein, acidic, cysteine-rich (osteonectin),
mRNA (cDNA clone MGC:3651 IMAGE:2906092), complete cds.
ACCESSION BC004974
VERSION BC004974.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2114)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2114)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (21-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: j Column: 4.
FEATURES Location/Qualifiers
source 1..2114
/db_xref="H-InvDB:HIT000032124"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3651 IMAGE:2906092"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2114
/gene="SPARC"
/db_xref="GeneID:6678"
/db_xref="HGNC:HGNC:11219"
/db_xref="MIM:182120"
CDS 51..962
/gene="SPARC"
/codon_start=1
/product="secreted protein, acidic, cysteine-rich
(osteonectin)"
/protein_id="AAH04974.1"
/db_xref="GeneID:6678"
/db_xref="HGNC:HGNC:11219"
/db_xref="MIM:182120"
/translation="MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVTEVSVG
ANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSEL
TEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLAR
DFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLD
NDKYIALDEWAGCFGIKQKDIDKDLVI"
BASE COUNT 529 a 533 c 516 g 536 t
ORIGIN
1 gcgcgctctg cctgccgcct gcctgcctgc cactgagggt tcccagcacc atgagggcct
61 ggatcttctt tctcctttgc ctggccggga gggccttggc agcccctcag caagaagccc
121 tgcctgatga gacagaggtg gtggaagaaa ctgtggcaga ggtgactgag gtatctgtgg
181 gagctaatcc tgtccaggtg gaagtaggag aatttgatga tggtgcagag gaaaccgaag
241 aggaggtggt ggcggaaaat ccctgccaga accaccactg caaacacggc aaggtgtgcg
301 agctggatga gaacaacacc cccatgtgcg tgtgccagga ccccaccagc tgcccagccc
361 ccattggcga gtttgagaag gtgtgcagca atgacaacaa gaccttcgac tcttcctgcc
421 acttctttgc cacaaagtgc accctggagg gcaccaagaa gggccacaag ctccacctgg
481 actacatcgg gccttgcaaa tacatccccc cttgcctgga ctctgagctg accgaattcc
541 ccctgcgcat gcgggactgg ctcaagaacg tcctggtcac cctgtatgag agggatgagg
601 acaacaacct tctgactgag aagcagaagc tgcgggtgaa gaagatccat gagaatgaga
661 agcgcctgga ggcaggagac caccccgtgg agctgctggc ccgggacttc gagaagaact
721 ataacatgta catcttccct gtacactggc agttcggcca gctggaccag caccccattg
781 acgggtacct ctcccacacc gagctggctc cactgcgtgc tcccctcatc cccatggagc
841 attgcaccac ccgctttttc gagacctgtg acctggacaa tgacaagtac atcgccctgg
901 atgagtgggc cggctgcttc ggcatcaagc agaaggatat cgacaaggat cttgtgatct
961 aaatccactc cttccacagt accggattct ctctttaacc ctccccttcg tgtttccccc
1021 aatgtttaaa atgtttggat ggtttgttgt tctgcctgga gacaaggtgc taacatagat
1081 ttaagtgaat acattaacgg tgctaaaaat gaaaattcta acccaagaca tgacattctt
1141 agctgtaact taactattaa ggccttttcc acacgcatta atagtcccat ttttctcttg
1201 ccatttgtag ctttgcccat tgtcttattg gcacatgggt ggacacggat ctgctgggct
1261 ctgccttaaa cacacattgc agcttcaact tttctcttta gtgttctgtt tgaaactaat
1321 acttaccgag tcagactttg tgttcatttc atttcagggt cttggctgcc tgtgggcttc
1381 cccaggtggc ctggaggtgg gcaaagggaa gtaacagaca cacgatgttg tcaaggatgg
1441 ttttgggact agaggctcag tggtgggaga gatccctgca gaacccacca accagaacgt
1501 ggtttgcctg aggctgtaac tgagagaaag attctggggc tgtcttatga aaatatagac
1561 attctcacat aagcccagtt catcaccatt tcctccttta cctttcagtg cagtttcttt
1621 tcacattagg ctgttggttc aaacttttgg gagcacggac tgtcagttct ctgggaagtg
1681 gtcagcgcat cctgcagggc ttctcctcct ctgtcttttg gagaaccagg gctcttctca
1741 ggggctctag ggactgccag gctgtttcag ccaggaaggc caaaatcaag agtgagatgt
1801 agaaagttgt aaaatagaaa aagtggagtt ggtgaatcgg ttgttctttc ctcacatttg
1861 gatgattgtc ataaggtttt tagcatgttc ctccttttct tcaccctccc cttttttctt
1921 ctattaatca agagaaactt caaagttaat gggatggtcg gatctcacag gctgagaact
1981 cgttcacctc caagcatttc atgaaaaagc tgcttcttat taatcataca aactctcacc
2041 atgatgtgaa gagtttcaca aatctttcaa aataaaaagt aatgacttag aaactgaaaa
2101 aaaaaaaaaa aaaa
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