LOCUS BC004929 2189 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and
steroid delta-isomerase 7, mRNA (cDNA clone MGC:10497
IMAGE:3626629), complete cds.
ACCESSION BC004929
VERSION BC004929.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2189)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2189)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (21-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 13 Row: c Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19923620.
FEATURES Location/Qualifiers
source 1..2189
/db_xref="H-InvDB:HIT000032092"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:10497 IMAGE:3626629"
/tissue_type="Uterus, endometrium adenocarcinoma"
/clone_lib="NIH_MGC_44"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2189
/gene="HSD3B7"
/gene_synonym="PFIC4"
/db_xref="GeneID:80270"
/db_xref="HGNC:HGNC:18324"
/db_xref="MIM:607764"
CDS 94..1203
/gene="HSD3B7"
/gene_synonym="PFIC4"
/codon_start=1
/product="hydroxy-delta-5-steroid dehydrogenase, 3 beta-
and steroid delta-isomerase 7"
/protein_id="AAH04929.1"
/db_xref="GeneID:80270"
/db_xref="HGNC:HGNC:18324"
/db_xref="MIM:607764"
/translation="MADSAQAQKLVYLVTGGCGFLGEHVVRMLLQREPRLGELRVFDQ
HLGPWLEELKTGPVRVTAIQGDVTQAHEVAAAVAGAHVVIHTAGLVDVFGRASPKTIH
EVNVQGTRNVIEACVQTGTRFLVYTSSMEVVGPNTKGHPFYRGNEDTPYEAVHRHPYP
CSKALAEWLVLEANGRKVRGGLPLVTCALRPTGIYGEGHQIMRDFYRQGLRLGGWLFR
AIPASVEHGRVYVGNVAWMHVLAARELEQRATLMGGQVYFCYDGSPYRSYEDFNMEFL
GPCGLRLVGARPLLPYWLLVFLAALNALLQWLLRPLVLYAPLLNPYTLAVANTTFTVS
TDKAQRHFGYEPLFSWEDSRTRTILWVQAATGSAQ"
BASE COUNT 401 a 678 c 657 g 453 t
ORIGIN
1 gaaggacggt gtgcgggccg gccagccctg gacgaaagaa gagggcccct ccaggccagt
61 ctgggcaccc tgggatagcg gctgcagcca ggcatggccg actctgcaca ggcccagaag
121 ctggtgtacc tggtcacagg gggctgtggc ttcctgggag agcacgtggt gcgaatgctg
181 ctgcagcggg agccccggct cggggagctg cgggtctttg accaacacct gggtccctgg
241 ctggaggagc tgaagacagg gcctgtgagg gtgactgcca tccaggggga cgtgacccag
301 gcccatgagg tggcagcagc tgtggccgga gcccatgtgg tcatccacac ggctgggctg
361 gtagacgtgt ttggcagggc cagtcccaag accatccatg aggtcaacgt gcagggtacc
421 cggaacgtga tcgaggcttg tgtgcagacc ggaacacggt tcctggtcta caccagcagc
481 atggaagttg tggggcctaa caccaaaggt caccccttct acaggggcaa cgaagacacc
541 ccatacgaag cagtgcacag gcacccctat ccttgcagca aggccctggc cgagtggctg
601 gtcctggagg ccaacgggag gaaggtccgt ggggggctgc ccctggtgac gtgtgccctt
661 cgtcccacgg gcatctacgg tgaaggccac cagatcatga gggacttcta ccgccagggc
721 ctgcgcctgg gaggttggct cttccgggcc atcccggcct ctgtggagca tggccgggtc
781 tatgtgggca atgttgcctg gatgcacgtg ctggcagccc gggagctgga gcagcgggca
841 accctgatgg gcggccaggt atacttctgc tacgatggat caccctacag gagctatgag
901 gatttcaaca tggagttcct gggcccctgc ggactgcggc tggtgggcgc ccgcccattg
961 ctgccctact ggctgctggt gttcctggct gccctcaatg ccctgctgca gtggctgctg
1021 cggccactgg tgctctacgc acccctgctg aacccctaca cgctggccgt ggccaacacc
1081 accttcaccg tcagcaccga caaggctcag cgccatttcg gctatgagcc cctgttctcg
1141 tgggaggata gccggacccg taccattctc tgggtacagg ccgctacggg ttcagcccag
1201 tgacggtggg gctggggcct ggaggcccag atacagcaca tccacccagg tcccgagccc
1261 tcacaccctg gacgggaagg gacagctgca ttccagagca ggaggcaggg ctctggggcc
1321 agaatggctg tccttgtcgt agagccctcc acattttctt tttctttttt gagacagggt
1381 cttgctctgt cacccagact ggagtgcagt ggtgtgatca tagctcactg caccctcaac
1441 ctcctgggtt caagcaatcc tcctgcctca gcctcctgaa cagctgggac cacaggtgca
1501 cgccaccaca cctggctttt ttttgttgtt tttagagaca gggtctcact atattgctca
1561 ggctggtctt gaactcctgg gctcaagtga tcttcccacg tgggcctccc aaaacgctgg
1621 aactacaagt gtgagccacc gcgcctggcc caagccctcc acattttcaa tccaggagcc
1681 ttgagtctgt gttgtgtcct gacacctcca agttctaggg ccgtcaggac acgggagggt
1741 ttggggacag agtgtccttc ctctgtcctc tcatcccagt cctgatggcc gcttggtgag
1801 tgtctggtgc cctggtggcc tgccccagct ctcttctggc tttctgagca ggaagcgagc
1861 agaggctcca caggcttacg ctgctctcct gacagccaca cgcgaccctc ggtgcagagt
1921 gcagaggcgg ctctggttcc tccagccacc tcagtccctc tttgggaggt gatgttccca
1981 ttgtttttca aaggcctcac cttcaactgt ctgtcttaga attcccctct ggagggctat
2041 ggcctcccta tgctttcact tcccacctct ctacctaagt tccttcccag cacatcgcca
2101 gccctgggcc tggggatgtc cccaatgctg tacctggctg accccggatt aaaagcctca
2161 tccacgaccg aaaaaaaaaa aaaaaaaaa
//