LOCUS BC004822 1851 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pseudouridylate synthase 3, mRNA (cDNA clone MGC:5252
IMAGE:3451112), complete cds.
ACCESSION BC004822
VERSION BC004822.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1851)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1851)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (21-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: f Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31542635.
FEATURES Location/Qualifiers
source 1..1851
/db_xref="H-InvDB:HIT000032035"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:5252 IMAGE:3451112"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1851
/gene="PUS3"
/gene_synonym="2610020J05Rik"
/gene_synonym="FKSG32"
/db_xref="GeneID:83480"
/db_xref="HGNC:HGNC:25461"
CDS 88..1533
/gene="PUS3"
/gene_synonym="2610020J05Rik"
/gene_synonym="FKSG32"
/codon_start=1
/product="pseudouridylate synthase 3"
/protein_id="AAH04822.1"
/db_xref="GeneID:83480"
/db_xref="HGNC:HGNC:25461"
/translation="MADNDTDRNQTEKLLKRVRELEQEVQRLKKEQAKNKEDSNIREN
SSGAGKTKRAFDFSAHGRRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTRL
VESRQTSNYHRCGRTDKGVSAFGQVISLDLRSQFPRGRDSEDFNVKEEANAAAEEIRY
THILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVTMDYAAQKYVG
THDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLY
HQVRCMMAILFLIGQGMEKPEIIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKW
IYDQEAQEFNITHLQQLWANHAVKTHMLYSMLQGLDTVPVPCGIGPKMDGMTEWGNVK
PSVIKQTSAFVEGVKMRTYKPLMDRPKCQGLESRIQHFVRRGRIEHPHLFHEEETKAK
RDCNDTLEEDNTNLETPTKRVCVDTEIKSII"
BASE COUNT 620 a 363 c 425 g 443 t
ORIGIN
1 gcttcctttc tcggaaacgc ggcgcggccg gctgccggaa aacagggcag acctgtatga
61 ttggtttatt cctggggttg tcatatcatg gctgataatg acacagacag aaaccagact
121 gagaagctcc taaaaagagt acgagaactg gagcaagagg tgcaaagact taaaaaggaa
181 caggccaaaa ataaggagga ctcaaacatt agagaaaatt catcaggagc tggaaaaact
241 aagcgtgcat ttgatttcag tgctcatggc cgaagacacg tagccctaag aatagcctat
301 atgggctggg gataccaggg ctttgctagt caggaaaaca caaataatac cattgaagag
361 aaactgtttg aagctctaac caagactcga ctagtagaaa gcagacagac atccaactat
421 caccgatgtg ggagaacaga taaaggagtt agtgcctttg gacaggtgat ctcacttgac
481 cttcgctctc agtttccaag gggcagggat tccgaggact ttaatgtaaa agaggaggct
541 aatgctgctg ctgaagagat ccgttatacc cacattctca atcgggtact ccctccagac
601 atccgtatat tggcctgggc ccctgtagaa ccaagcttca gtgctaggtt cagctgcctt
661 gagcggactt accgctattt tttccctcgt gctgatttag atattgtaac catggattat
721 gcagctcaga agtatgttgg cacccatgat ttcaggaact tgtgtaaaat ggatgtagcc
781 aacggtgtga ttaattttca gaggactatt ctatctgctc aagtacagct agtgggccag
841 agcccaggtg aggggagatg gcaagaacct ttccagttat gtcagtttga agtgactggc
901 caggcattcc tttatcatca agtccgatgt atgatggcta tcctctttct gattggccaa
961 ggaatggaga agccagagat tattgatgag ctgctgaata tagagaaaaa tccccaaaag
1021 cctcaatata gtatggctgt agaatttcct ctagtcttat atgactgtaa gtttgaaaat
1081 gtcaagtgga tctatgacca ggaggctcag gagttcaata ttacccacct acaacaactg
1141 tgggctaatc atgctgtcaa aactcacatg ttgtatagta tgctacaagg actggacact
1201 gttccagtac cctgtggaat aggaccaaag atggatggaa tgacagaatg gggaaatgtt
1261 aagccctctg tcataaagca gaccagtgcc tttgtagaag gagtgaagat gcgcacatat
1321 aagcccctca tggaccgtcc taaatgccaa ggactggaat cccggatcca gcattttgta
1381 cgtaggggac gaattgagca cccacattta ttccatgagg aagaaacaaa agccaaaagg
1441 gactgtaatg acacactaga ggaagacaat actaatttgg agacaccaac gaagagggtc
1501 tgtgttgaca cagaaattaa aagcatcatt taaccataga caatttgcca ggatctagga
1561 accacctaat ggtaggtgga cagaaaagga aaaaaaaaaa aatttacttg caagtactag
1621 gaattcagat gatcagctct taaaaaaaaa aaaaaagcaa aaagactaaa gccctattaa
1681 ggaagttatt gctttaataa gaaatttcaa atattctctt atcccggtcc aaaaggatta
1741 agcgattaaa gaacgtaaaa tggagatgta tttacataca cctggaaacc tgtgccttgt
1801 attcaaattc attaaagcct aatcctgcaa gtaaaaaaaa aaaaaaaaaa a
//