LOCUS BC004451 936 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens RPA interacting protein, mRNA (cDNA clone MGC:4189
IMAGE:2820882), complete cds.
ACCESSION BC004451
VERSION BC004451.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 936)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 936)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: k Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14149614
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..936
/db_xref="H-InvDB:HIT000031956"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4189 IMAGE:2820882"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..936
/gene="RPAIN"
/gene_synonym="HRIP"
/gene_synonym="RIP"
/db_xref="GeneID:84268"
/db_xref="HGNC:HGNC:28641"
CDS 14..451
/gene="RPAIN"
/gene_synonym="HRIP"
/gene_synonym="RIP"
/codon_start=1
/product="RPAIN protein"
/protein_id="AAH04451.1"
/db_xref="GeneID:84268"
/db_xref="HGNC:HGNC:28641"
/translation="MAESLRSPRRSLYKLVGSPPWKEAFRQRCLERMRNSRDRLLNRY
RQAGSSGPGNSQNSFLVQEVMEEEWNALQSVENCPEDLAQLEELIDMAVLEEIQQELI
NQEQSIISEYEKSLQFDEKCLSIMLAEWEANPLICPVCTNLLS"
BASE COUNT 296 a 181 c 236 g 223 t
ORIGIN
1 ccgcgaagag gagatggcgg agtcgttgag gtctccgcgc cgctccctgt acaaactggt
61 gggctcgccg ccttggaaag aggctttccg gcagagatgc ctggagagaa tgagaaacag
121 ccgggacagg ctcctaaaca ggtaccgcca ggctggaagc agtgggccag ggaattctca
181 gaacagcttt ctagttcaag aggtgatgga agaagagtgg aatgctttgc agtcagtgga
241 gaattgtcca gaagacttgg ctcagctgga ggagctgata gacatggctg tgctggagga
301 aattcaacag gagctgatca accaagagca gtccatcatc agcgagtatg agaagagctt
361 gcagtttgat gaaaagtgtc tcagcatcat gctggctgag tgggaggcaa acccactcat
421 ctgtcctgta tgtacaaatc ttctgagttg acagagcaga agcttcgtgc ctgtttagag
481 ggtagtataa atgagcacag tgcacattgt ccccacacac ctgaattttc agtcactgga
541 ggaacagaag aaaagtccag tcttctcatg agctgtctgg cctgtgatac ttgggctgtg
601 atcctctaga gccagcttgg actcacatca ttctatgggg ttgaagacaa ctcattccct
661 ctgaggagcc ttgtacatac aagcctttta tttataactt attttgtatt gaaactttta
721 aacaatactg aagaaaaaaa aacttttccg acatctgttc ttggtctttt gtgacgcagg
781 ttgaaggggg aggaatagaa aaagacaaac tgccttggag gagataaacc aattttatgt
841 ctatcatgtt atacaaaaat ctagaaataa tagatttgta cagaaaaaaa tgataataaa
901 tgagaacaca aaccaaaaaa aaaaaaaaaa aaaaaa
//