LOCUS BC004253 1393 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens keratin 18, mRNA (cDNA clone MGC:10564 IMAGE:3631540),
complete cds.
ACCESSION BC004253
VERSION BC004253.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1393)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1393)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 13 Row: e Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40354194.
FEATURES Location/Qualifiers
source 1..1393
/db_xref="H-InvDB:HIT000031820"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:10564 IMAGE:3631540"
/tissue_type="Uterus, endometrium adenocarcinoma"
/clone_lib="NIH_MGC_44"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1393
/gene="KRT18"
/gene_synonym="CYK18"
/gene_synonym="K18"
/db_xref="GeneID:3875"
/db_xref="HGNC:HGNC:6430"
/db_xref="MIM:148070"
CDS 27..1319
/gene="KRT18"
/gene_synonym="CYK18"
/gene_synonym="K18"
/codon_start=1
/product="keratin 18"
/protein_id="AAH04253.1"
/db_xref="GeneID:3875"
/db_xref="HGNC:HGNC:6430"
/db_xref="MIM:148070"
/translation="MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGS
RISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLE
TENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLA
ADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHE
EEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIE
ESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQ
MEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNL
GDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH"
BASE COUNT 361 a 382 c 417 g 233 t
ORIGIN
1 ctgtcctttc tctctccccg gacagcatga gcttcaccac tcgctccacc ttctccacca
61 actaccggtc cctgggctct gtccaggcgc ccagctacgg cgcccggccg gtcagcagcg
121 cggccagcgt ctatgcaggc gctgggggct ctggttcccg gatctccgtg tcccgctcca
181 ccagcttcag gggcggcatg gggtccgggg gcctggccac cgggatagcc gggggtctgg
241 caggaatggg aggcatccag aacgagaagg agaccatgca aagcctgaac gaccgcctgg
301 cctcttacct ggacagagtg aggagcctgg agaccgagaa ccggaggctg gagagcaaaa
361 tccgggagca cttggagaag aagggacccc aggtcagaga ctggagccat tacttcaaga
421 tcatcgagga cctgagggct cagatcttcg caaatactgt ggacaatgcc cgcatcgttc
481 tgcagattga caatgcccgt cttgctgctg atgactttag agtcaagtat gagacagagc
541 tggccatgcg ccagtctgtg gagaacgaca tccatgggct ccgcaaggtc attgatgaca
601 ccaatatcac acgactgcag ctggagacag agatcgaggc tctcaaggag gagctgctct
661 tcatgaagaa gaaccacgaa gaggaagtaa aaggcctaca agcccagatt gccagctctg
721 ggttgaccgt ggaggtagat gcccccaaat ctcaggacct cgccaagatc atggcagaca
781 tccgggccca atatgacgag ctggctcgga agaaccgaga ggagctagac aagtactggt
841 ctcagcagat tgaggagagc accacagtgg tcaccacaca gtctgctgag gttggagctg
901 ctgagacgac gctcacagag ctgagacgta cagtccagtc cttggagatc gacctggact
961 ccatgagaaa tctgaaggcc agcttggaga acagcctgag ggaggtggag gcccgctacg
1021 ccctacagat ggagcagctc aacgggatcc tgctgcacct tgagtcagag ctggcacaga
1081 cccgggcaga gggacagcgc caggcccagg agtatgaggc cctgctgaac atcaaggtca
1141 agctggaggc tgagatcgcc acctaccgcc gcctgctgga agatggcgag gactttaatc
1201 ttggtgatgc cttggacagc agcaactcca tgcaaaccat ccaaaagacc accacccgcc
1261 ggatagtgga tggcaaagtg gtgtctgaga ccaatgacac caaagttctg aggcattaag
1321 ccagcagaag cagggtaccc tttggggagc aggaggccaa taaaaagttc agagttaaaa
1381 aaaaaaaaaa aaa
//