LOCUS BC004132 1988 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens lysyl-tRNA synthetase, mRNA (cDNA clone MGC:1404
IMAGE:2963032), complete cds.
ACCESSION BC004132
VERSION BC004132.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1988)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1988)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Sep 16, 2003 this sequence version replaced BC004132.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 2 Row: l Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5031814.
FEATURES Location/Qualifiers
source 1..1988
/db_xref="H-InvDB:HIT000031721"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1404 IMAGE:2963032"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1988
/gene="KARS"
/gene_synonym="KARS2"
/gene_synonym="KIAA0070"
/db_xref="GeneID:3735"
/db_xref="HGNC:HGNC:6215"
/db_xref="MIM:601421"
CDS 12..1805
/gene="KARS"
/gene_synonym="KARS2"
/gene_synonym="KIAA0070"
/codon_start=1
/product="lysyl-tRNA synthetase"
/protein_id="AAH04132.1"
/db_xref="GeneID:3735"
/db_xref="HGNC:HGNC:6215"
/db_xref="MIM:601421"
/translation="MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELS
EKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVD
ISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANS
RNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHF
GLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPG
GAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPE
FTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFR
RINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGE
FLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQ
RQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKE
VLLFPAMKPEDKKENVATTDTLESTTVGTSV"
BASE COUNT 600 a 447 c 486 g 455 t
ORIGIN
1 cctccgggaa gatggcggcc gtgcaggcgg ccgaggtgaa agtggatggc agcgagccga
61 aactgagcaa gaatgagctg aagagacgcc tgaaagctga gaagaaagta gcagagaagg
121 aggccaaaca gaaggagctc agtgagaaac agctaagcca agccactgct gctgccacca
181 accacaccac tgataatggt gtgggtcctg aggaagagag cgtggaccca aatcaatact
241 acaaaatccg cagtcaagca attcatcagc tgaaggtcaa tggggaagac ccatacccac
301 acaagttcca tgtagacatc tcactcactg acttcatcca aaaatatagt cacctgcagc
361 ctggggatca cctgactgac atcaccttaa aggtggcagg taggatccat gccaaaagag
421 cttctggggg aaagctcatc ttctatgatc ttcgaggaga gggggtgaag ttgcaagtca
481 tggccaattc cagaaattat aaatcagaag aagaatttat tcatattaat aacaaactgc
541 gtcggggaga cataattgga gttcagggga atcctggtaa aaccaagaag ggtgagctga
601 gcatcattcc gtatgagatc acactgctgt ctccctgttt gcatatgtta cctcatcttc
661 actttggcct caaagacaag gaaacaaggt atcgccagag atacttggac ttgatcctga
721 atgactttgt gaggcagaaa tttatcatcc gctctaagat catcacatat ataagaagtt
781 tcttagatga gctgggattc ctagagattg aaactcccat gatgaacatc atcccagggg
841 gagccgtggc caagcctttc atcacttatc acaacgagct ggacatgaac ttatatatga
901 gaattgctcc agaactctat cataagatgc ttgtggttgg tggcatcgac cgggtttatg
961 aaattggacg ccagttccgg aatgagggga ttgatttgac gcacaatcct gagttcacca
1021 cctgtgagtt ctacatggcc tatgcagact atcacgatct catggaaatc acggagaaga
1081 tggtttcagg gatggtgaag catattacag gcagttacaa ggtcacctac cacccagatg
1141 gcccagaggg ccaagcctac gatgttgact tcaccccacc cttccggcga atcaacatgg
1201 tagaagagct tgagaaagcc ctggggatga agctgccaga aacgaacctc tttgaaactg
1261 aagaaactcg caaaattctt gatgatatct gtgtggcaaa agctgttgaa tgccctccac
1321 ctcggaccac agccaggctc cttgacaagc ttgttgggga gttcctggaa gtgacttgca
1381 tcaatcctac attcatctgt gatcacccac agataatgag ccctttggct aaatggcacc
1441 gctctaaaga gggtctgact gagcgctttg agctgtttgt catgaagaaa gagatatgca
1501 atgcgtatac tgagctgaat gatcccatgc ggcagcggca gctttttgaa gaacaggcca
1561 aggccaaggc tgcaggtgat gatgaggcca tgttcataga tgaaaacttc tgtactgccc
1621 tggaatatgg gctgcccccc acagctggct ggggcatggg cattgatcga gtcgccatgt
1681 ttctcacgga ctccaacaac atcaaggaag tacttctgtt tcctgccatg aaacccgaag
1741 acaagaagga gaatgtagca accactgata cactggaaag cacaacagtt ggcacttctg
1801 tctagaaaat aataattgca agttgtataa ctcaggcgtc tttgcatttc tgcgaaagat
1861 caaggtctgc aagggaattc ttgtgtgctg ctttccattt gacaccgcag ttctgttcag
1921 ccatcagaag agagacaagg aattaaaaat ttctttttaa tcctgttacc aaaaaaaaaa
1981 aaaaaaaa
//