LOCUS BC003643 1504 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens dehydrodolichyl diphosphate synthase, mRNA (cDNA clone
MGC:4674 IMAGE:3532466), complete cds.
ACCESSION BC003643
VERSION BC003643.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1504)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1504)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC003643.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: p Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 45580737.
FEATURES Location/Qualifiers
source 1..1504
/db_xref="H-InvDB:HIT000031667"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4674 IMAGE:3532466"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1504
/gene="DHDDS"
/gene_synonym="CIT"
/gene_synonym="CPT"
/gene_synonym="DS"
/gene_synonym="FLJ13102"
/gene_synonym="HDS"
/db_xref="GeneID:79947"
/db_xref="HGNC:HGNC:20603"
/db_xref="MIM:608172"
CDS 56..1057
/gene="DHDDS"
/gene_synonym="CIT"
/gene_synonym="CPT"
/gene_synonym="DS"
/gene_synonym="FLJ13102"
/gene_synonym="HDS"
/codon_start=1
/product="dehydrodolichyl diphosphate synthase"
/protein_id="AAH03643.1"
/db_xref="GeneID:79947"
/db_xref="HGNC:HGNC:20603"
/db_xref="MIM:608172"
/translation="MSWIKEGELSLWERFCANIIKAGPMPKHIAFIMDGNRRYAKKCQ
VERQEGHSQGFNKLAETLRWCLNLGILEVTVYAFSIENFKRSKSEVDGLMDLARQKFS
RLMEEKEKLQKHGVCIRVLGDLHLLPLDLQELIAQAVQATKNYNKCFLNVCFAYTSRH
EISNAVREMAWGVEQGLLDPSDISESLLDKCLYTNRSPHPDILIRTSGEVRLSDFLLW
QTSHSCLVFQPVLWPEYTFWNLFEAILQFQMNHSMLQKARDMYAEERKRQQLERDQAT
VTEQLLREGLQASGDAQLRRTRLHKLSARREERVQGFLQALELKRADWLARLGTASA"
BASE COUNT 360 a 394 c 405 g 345 t
ORIGIN
1 attacctggc tggtgtttgc ttgttctgga gtgatcttct gactggaaaa gaactatgtc
61 atggatcaag gaaggagagc tgtcactttg ggagcggttc tgtgccaaca tcataaaggc
121 aggcccaatg ccgaaacaca ttgcattcat aatggacggg aaccgtcgct atgccaagaa
181 gtgccaggtg gagcggcagg aaggccactc acagggcttc aacaagctag ctgagactct
241 gcggtggtgt ttgaacctgg gcatcctaga ggtgacagtc tacgcattca gcattgagaa
301 cttcaaacgc tccaagagtg aggtagacgg gcttatggat ctggcccggc agaagttcag
361 ccgcttgatg gaagaaaagg agaaactgca gaagcatggg gtgtgtatcc gggtcctggg
421 cgatctgcac ttgttgccct tggatctcca ggagctgatt gcacaagctg tacaggccac
481 gaagaactac aacaagtgtt tcctgaatgt ctgttttgca tacacatccc gtcatgagat
541 cagcaatgct gtgagagaga tggcctgggg ggtggagcaa ggcctgttgg atcccagtga
601 tatctctgag tctctgcttg ataagtgcct ctataccaac cgctctcctc atcctgacat
661 cttgatacgg acttctggag aagtgcggct gagtgacttc ttgctatggc agacctctca
721 ctcctgcctg gtgttccaac ccgttctgtg gccagagtat acattttgga acctcttcga
781 ggccatcctg cagttccaga tgaaccatag catgcttcag aaggcccgag acatgtatgc
841 agaggagcgg aagaggcagc agctggagag ggaccaggct acagtgacag agcagctgct
901 gcgagagggg ctccaagcca gtggggacgc ccagctccga aggacacgct tgcacaaact
961 ctcggccaga cgggaagagc gagtccaagg cttcctgcag gccttggaac tcaagcgagc
1021 tgactggctg gcccgtctgg gcactgcatc agcctgaatg aggctggcca cctgccactt
1081 tgccctgccc tctgcctcca gggctccact ccccttcctt ttcttggtga aaggcacctc
1141 ctttcctgat aatgaatggt gttccctttg cttggctggg gagcccccca ggccaggttt
1201 gctggccata gatacctttg ggctgcctgg gacaggctcc tgaggaggat tgagggtgaa
1261 agtctcccac gagtacacta aacctaggtc tggtcaccaa tagggtttgg agagcaaagg
1321 gccacaactc atcagctgcc tgtctcttag atgcactttc tttttccacc agcacatcct
1381 tcaacacaca gaatttcagg gaagagttct ccccaaaacc ctagctcttt acccttccat
1441 tttagccttc cacccagctt ccacaaaaga tttggctcta ccttggaaaa aaaaaaaaaa
1501 aaaa
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