LOCUS BC003395 1450 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog,
yeast), mRNA (cDNA clone MGC:5416 IMAGE:3447673), complete cds.
ACCESSION BC003395
VERSION BC003395.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1450)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1450)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (20-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: g Column: 20.
FEATURES Location/Qualifiers
source 1..1450
/db_xref="H-InvDB:HIT000031542"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:5416 IMAGE:3447673"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1450
/gene="UBE2D3"
/gene_synonym="E2(17)KB3"
/gene_synonym="MGC43926"
/gene_synonym="MGC5416"
/gene_synonym="UBC4/5"
/gene_synonym="UBCH5C"
/db_xref="GeneID:7323"
/db_xref="HGNC:HGNC:12476"
/db_xref="MIM:602963"
CDS 166..609
/gene="UBE2D3"
/gene_synonym="E2(17)KB3"
/gene_synonym="MGC43926"
/gene_synonym="MGC5416"
/gene_synonym="UBC4/5"
/gene_synonym="UBCH5C"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2D 3 (UBC4/5
homolog, yeast)"
/protein_id="AAH03395.1"
/db_xref="GeneID:7323"
/db_xref="HGNC:HGNC:12476"
/db_xref="MIM:602963"
/translation="MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSP
YQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKV
LLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM"
BASE COUNT 445 a 289 c 278 g 438 t
ORIGIN
1 cacgaagttc tcgcgagagt cgtctcctcg ataccaagcg cctgtgtctg gcagagctgg
61 tgtgagacga gacaatcctg ccccgccgcc gggataatca agagttttgg ccggaccttt
121 gagcatacac cgagagagtg aggagccaga cgacaagcac acactatggc gctgaaacgg
181 attaataagg aacttagtga tttggcccgt gaccctccag cacaatgttc tgcaggtcca
241 gttggggatg atatgtttca ttggcaagcc acaattatgg gacctaatga cagcccatat
301 caaggcggtg tattcttttt gacaattcat tttcctacag actacccctt caaaccacct
361 aaggttgcat ttacaacaag aatttatcat ccaaatatta acagtaatgg cagcatttgt
421 ctcgatattc taagatcaca gtggtcgcct gctttaacaa tttctaaagt tcttttatcc
481 atttgttcac tgctatgtga tccaaaccca gatgaccccc tagtgccaga gattgcacgg
541 atctataaaa cagacagaga taagtacaac agaatatctc gggaatggac tcagaagtat
601 gccatgtgat gctaccttaa agtcagaata acctgcatta tagctggaat aaactttaaa
661 ttactgttcc ttttttgatt ttcttatccg gctgctcccc tatcagacct catctttttt
721 aattttattt tttgtttacc tccctccatt cattcacatg ctcatctgag aagacttaag
781 ttcttccagc tttggacaat aactgctttt agaaactgta aagtagttac aagagaacag
841 ttgcccaaga ctcagaattt ttaaaaaaaa aaatggagca tgtgtattat gtggccaatg
901 tcttcactct aacttggtta tgagactaaa accattcctc actgctctaa catgctgaag
961 aaatcatctg agggggaggg agatggatgc tcagttgtca catcaaagga tacagcatta
1021 ttctagcagc atccattctt gtttaagcct tccactgtta gagatttgag gttacatgat
1081 atgctttatg ctcataactg atgtggctgg agaattggta ttgaatttat agcatcagca
1141 gaacagaaaa tgtgatgtat tttatgcatg tcaataaagg aatgacctgt tcttgttcta
1201 cagagaatgg aaattggaag tcaaacaccc tttgtattcc aaaatagggt ctcaaacatt
1261 ttgtaatttt catttaaatt gttaggaggc ttggagctat tagttaatct atcttccaat
1321 acactgttta atatagcact gaataaatga tgcaagttgt caatggatga gtgatcaact
1381 aatagctctg ctagtaattg atttattttt cttcaataaa gttgcataaa ccaaaaaaaa
1441 aaaaaaaaaa
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