LOCUS       BC003370                 609 bp    mRNA    linear   HUM 15-JUL-2006
DEFINITION  Homo sapiens cystatin B (stefin B), mRNA (cDNA clone MGC:5180
            IMAGE:2900656), complete cds.
ACCESSION   BC003370
VERSION     BC003370.1
KEYWORDS    MGC.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2001) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: ATCC
            cDNA Library Preparation: Life Technologies, Inc.
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Baylor College of Medicine Human Genome
            Sequencing Center
            Center code: BCM-HGSC
            Web site: http://www.hgsc.bcm.tmc.edu/cdna/
            Contact: amg@bcm.tmc.edu
            Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
            Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
            A.N., Gibbs, R.A.
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAK Plate: 3 Row: g Column: 17
            This clone was selected for full length sequencing because it
            passed the following selection criteria: matched mRNA gi: 20357564.
FEATURES             Location/Qualifiers
     source          1..609
                     /db_xref="H-InvDB:HIT000031523"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:5180 IMAGE:2900656"
                     /tissue_type="Placenta, choriocarcinoma"
                     /clone_lib="NIH_MGC_10"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6"
     gene            1..609
                     /gene="CSTB"
                     /gene_synonym="CST6"
                     /gene_synonym="cystatin B"
                     /gene_synonym="PME"
                     /db_xref="GeneID:1476"
                     /db_xref="HGNC:HGNC:2482"
                     /db_xref="MIM:601145"
     CDS             45..341
                     /gene="CSTB"
                     /gene_synonym="CST6"
                     /gene_synonym="cystatin B"
                     /gene_synonym="PME"
                     /codon_start=1
                     /product="cystatin B (stefin B)"
                     /protein_id="AAH03370.1"
                     /db_xref="GeneID:1476"
                     /db_xref="HGNC:HGNC:2482"
                     /db_xref="MIM:601145"
                     /translation="MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFK
                     SQVVAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF"
BASE COUNT          161 a          170 c          130 g          148 t
ORIGIN      
        1 ccctcgccga gtcccctcgc cagattccct ccgtcgccgc caagatgatg tgcggggcgc
       61 cctccgccac gcagccggcc accgccgaga cccagcacat cgccgaccag gtgaggtccc
      121 agcttgaaga gaaagaaaac aagaagttcc ctgtgtttaa ggccgtgtca ttcaagagcc
      181 aggtggtcgc ggggacaaac tacttcatca aggtgcacgt cggcgacgag gacttcgtac
      241 acctgcgagt gttccaatct ctccctcatg aaaacaagcc cttgacctta tctaactacc
      301 agaccaacaa agccaagcat gatgagctga cctatttctg atcctgactt tggacaaggc
      361 ccttcagcca gaagactgac aaagtcatcc tccgtctacc agagcgtgca cttgtgatcc
      421 taaaataagc ttcatctccg ggctgtgccc cttggggtgg aaggggcagg attctgcagc
      481 tgcttttgca tttctcttcc taaatttcat tgtgttgatt tctttccttc ccaataggtg
      541 atcttaatta ctttcagaat attttcaaaa tagatatatt tttaaaatcc ttaaaaaaaa
      601 aaaaaaaaa
//