LOCUS BC003365 1185 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2N (UBC13 homolog,
yeast), mRNA (cDNA clone MGC:5063 IMAGE:2900313), complete cds.
ACCESSION BC003365
VERSION BC003365.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1185)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1185)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (20-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: c Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 61175264.
FEATURES Location/Qualifiers
source 1..1185
/db_xref="H-InvDB:HIT000031519"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:5063 IMAGE:2900313"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1185
/gene="UBE2N"
/gene_synonym="MGC8489"
/gene_synonym="UBC13"
/gene_synonym="UbcH-ben"
/db_xref="GeneID:7334"
/db_xref="HGNC:HGNC:12492"
/db_xref="MIM:603679"
CDS 28..486
/gene="UBE2N"
/gene_synonym="MGC8489"
/gene_synonym="UBC13"
/gene_synonym="UbcH-ben"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2N (UBC13 homolog,
yeast)"
/protein_id="AAH03365.1"
/db_xref="GeneID:7334"
/db_xref="HGNC:HGNC:12492"
/db_xref="MIM:603679"
/translation="MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQD
SPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIR
TVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI"
BASE COUNT 344 a 243 c 243 g 355 t
ORIGIN
1 cagaggccga actcgggttc tgacaagatg gccgggctgc cccgcaggat catcaaggaa
61 acccagcgtt tgctggcaga accagttcct ggcatcaaag ccgaaccaga tgagagcaac
121 gcccgttatt ttcatgtggt cattgctggc cctcaggatt ccccctttga gggagggact
181 tttaaacttg aactattcct tccagaagaa tacccaatgg cagcccctaa agtacgtttc
241 atgaccaaaa tttatcatcc taatgtagac aagttgggaa gaatatgttt agatattttg
301 aaagataagt ggtccccagc actgcagatc cgcacagttc tgctatcgat ccaggccttg
361 ttaagtgctc ccaatccaga tgatccatta gcaaatgatg tagcggagca gtggaagacc
421 aacgaagccc aagccataga aacagctaga gcatggacta ggctatatgc catgaataat
481 atttaaattg atacgatcat caagtgtgca tcacttctcc tgttctgcca agacttcctc
541 ctctttgttt gcatttaatg gacacagtct tagaaacatt acagaataaa aaagcccaga
601 catcttcagt cctttggtga ttaaatgcac attagcaaat ctatgtcttg tcctgattca
661 ctgtcataaa gcatgagcag aggctagaag tatcatctgg attgttgtga aacgtttaaa
721 agcagtggcc cctccctgct tttattcatt tcccccatcc tggtttaagt ataaagcact
781 gtgaatgaag gtagttgtca ggttagctgc aggggtgtgg gtgtttttat tttattttat
841 tttattttat ttttgagggg ggaggtagtt taattttatg ggctcctttc cccctttttt
901 ggtgatctaa ttgcattggt taaaagcagc taaccaggtc tttagaatat gctctagcca
961 agtctaactt tatttagacg ctgtagatgg acaagcttga ttgttggaac caaaatggga
1021 acattaaaca aacatcacag ccctcactaa taacattgct gtcaagtgta gattcccccc
1081 ttcaaaaaaa gcttgtgacc attttgtatg gcttgtctgg aaacttctgt aaatcttatg
1141 ttttagtaaa atattttttg ttattctaaa aaaaaaaaaa aaaaa
//