LOCUS BC002990 2514 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens polymerase (DNA directed), alpha 2 (70kD subunit),
mRNA (cDNA clone MGC:3675 IMAGE:2822514), complete cds.
ACCESSION BC002990
VERSION BC002990.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2514)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2514)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: j Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 20127447.
FEATURES Location/Qualifiers
source 1..2514
/db_xref="H-InvDB:HIT000031339"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3675 IMAGE:2822514"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2514
/gene="POLA2"
/gene_synonym="FLJ21662"
/db_xref="GeneID:23649"
/db_xref="HGNC:HGNC:30073"
CDS 343..2139
/gene="POLA2"
/gene_synonym="FLJ21662"
/codon_start=1
/product="polymerase (DNA directed), alpha 2 (70kD
subunit)"
/protein_id="AAH02990.1"
/db_xref="GeneID:23649"
/db_xref="HGNC:HGNC:30073"
/translation="MSASAQQLAEELQIFGLDCEEALIEKLVELCVQYGQNEEGMVGE
LIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEV
EEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQK
YNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIRE
VLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGDR
EHSSGAQIPVDLSELKEYSLFPGQVVIMEGINTTGRKLVATKLYEGVPLPFYQPTEED
ADFEQSMVLVACGPYTTSDSITYDPLLDLIAVINHDRPDVCILFGPFLDAKHEQVENC
LLTSPFEDIFKQCLRTIIEGTRSSGSHLVFVPSLRDVHHEPVYPQPPFSYSDLSREDK
KQVQFVSEPCSLSINGVIFGLTSTDLLFHLGAEEISSSSGTSDRFSRILKHILTQRSY
YPLYPPQEDMAIDYESFYVYAQLPVTPDVLIIPSELRYFVKDVLGCVCVNPGRLTKGQ
VGGTFARLYLRRPAADGAERQSPCIAVQVVRI"
BASE COUNT 628 a 653 c 662 g 571 t
ORIGIN
1 cctcgtgccg cagttctgcc accgtcactg agaagctcag cggtagcttt tgggaagcag
61 gacgttctca ccaggagagc gtcctctcga gatttctgct ccctccattc agggcgtttg
121 ggagccaccc cttcattttt taaaaaaagt atttctctgt gaccgacggc cggggccttc
181 tgacggtctg aggtcttgct tgggccagtc acctcctgtc acggtccgcg gaggggggaa
241 ggataagagg gcgaggagct catcgctcgc cacccccgtg ggcttcttgg gcgcaggtcg
301 gagctgggtg ggccggctcc ccggcccctg gcttgggcga ccatgtccgc atccgcccag
361 cagctggcgg aggagctgca gatcttcggc ctagactgcg aggaggctct aattgagaaa
421 ttggtagagc tttgtgttca gtatggacag aatgaggagg gaatggtagg cgagcttata
481 gccttctgca ccagcacaca taaagttggc cttacctcag agatcctgaa ctcttttgag
541 catgagtttc tgagcaaaag attatcgaaa gccaggcata gtacctgcaa ggacagtggc
601 catgcaggag ctagagacat tgtttccatt caagagctaa ttgaagtgga agaagaagag
661 gaaatcctct tgaactctta caccacacct tcaaagggtt ctcagaagcg agctatctct
721 accccagaaa cccccctaac aaaaaggagt gtgtcaactc gtagccccca tcagctactc
781 tcaccgtcaa gtttctctcc aagtgctact ccctcccaga aatacaactc acgaagtaac
841 cgaggagaag tggttacctc cttcggctta gcacagggag tatcttggtc tgggagagga
901 ggagctggaa acatcagcct gaaggtcttg ggatgtccag aggcactaac tgggagctac
961 aaatccatgt ttcagaagct cccagacatt cgagaagttc tgacctgtaa gatagaagaa
1021 cttggcagcg aactcaagga acattacaag attgaagctt tcactccttt gctagcccca
1081 gcacaggagc ctgtcactct gctgggccag attggctgtg atagcaacgg gaagctgaac
1141 aacaagtcag tgattctcga gggagaccgg gaacattcct cgggtgctca aattccagtg
1201 gatttatctg agcttaagga atattctctg tttcctggac aggttgtaat tatggaagga
1261 atcaacacca ctggtaggaa acttgttgcc accaaactct acgagggtgt gccacttcca
1321 ttttatcagc ccactgaaga ggatgcagac tttgagcaaa gcatggtcct ggttgcctgt
1381 ggaccataca ccacatctga cagcatcacg tatgaccccc tgcttgacct gattgctgtc
1441 atcaaccatg accggccaga tgtctgcatc ctgtttggcc ctttcctgga tgctaagcat
1501 gaacaggtgg agaattgtct actgacaagt ccatttgaag acattttcaa gcagtgtcta
1561 cgaacaatta ttgaaggcac aagaagctcc ggctcccacc ttgtctttgt cccgtcattg
1621 agagatgtgc accatgagcc tgtgtacccc cagccgcctt tcagctactc cgatctgtct
1681 cgagaggaca aaaagcaagt acagtttgtg tccgagccct gcagcctctc cataaacgga
1741 gtgatcttcg gcttgacatc cacagatctg cttttccacc tgggggccga ggagatcagt
1801 agttcttccg gaacttcaga cagattcagc cgaatactca agcacatctt gacccagagg
1861 agctactacc cactctaccc gccccaagaa gacatggcca ttgactatga gtcgttctat
1921 gtttacgcac agctgcctgt caccccagat gtcctcatca tcccgtcaga gctgaggtac
1981 ttcgtgaagg atgtcctcgg ctgtgtctgt gtgaaccctg ggcgccttac caaagggcag
2041 gtgggaggca ccttcgcccg actctacctt aggaggccgg cagcggacgg ggcagagagg
2101 cagagcccat gcattgctgt gcaggtcgtc aggatctgag gcttctgtcc tctgctgttc
2161 tctgctgtgt gggcccttaa agtcttagcc aagagccaag acatagccct gtgacaaggt
2221 gaacagttgg gtgggaaagg agagaggagc cagccaggga ggggcagctg cagtgaccag
2281 gcccagcagg gaggacttgt gcagccgggc ctgcctctga gtggtgcctc tcctggaagg
2341 aagctcttgc ttctcagtcc atgctccgtg tccagaagta agccagctgt ggatcccgcc
2401 cactcagaaa aggcgagaag gctttgtgat tttctacatg aatcaaacac agaaacaact
2461 tttggagaaa ttaaattctg agtgtgaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//