LOCUS BC002582 3331 bp mRNA linear HUM 04-AUG-2008
DEFINITION Homo sapiens ubiquitin protein ligase E3A, mRNA (cDNA clone
MGC:2086 IMAGE:3160225), complete cds.
ACCESSION BC002582
VERSION BC002582.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3331)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3331)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Oct 8, 2003 this sequence version replaced BC002582.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 4 Row: d Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19718763.
FEATURES Location/Qualifiers
source 1..3331
/db_xref="H-InvDB:HIT000031010"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2086 IMAGE:3160225"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3331
/gene="UBE3A"
/gene_synonym="ANCR"
/gene_synonym="AS"
/gene_synonym="E6-AP"
/db_xref="GeneID:7337"
/db_xref="HGNC:HGNC:12496"
/db_xref="MIM:601623"
CDS 658..3276
/gene="UBE3A"
/gene_synonym="ANCR"
/gene_synonym="AS"
/gene_synonym="E6-AP"
/codon_start=1
/product="ubiquitin protein ligase E3A"
/protein_id="AAH02582.2"
/db_xref="GeneID:7337"
/db_xref="HGNC:HGNC:12496"
/db_xref="MIM:601623"
/translation="MATACKRSGEPQSDDIEASRMKRAAAKHLIERYYHQLTEGCGNE
ACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGAPN
NSCSEIKMNKKGARIDFKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEAL
VQSFRKVKQHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGD
NNLQKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSR
DPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYNADQIR
RMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEE
DDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCRKPLIPFEEFINEP
LNEVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLY
SLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGG
VSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNC
ILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQT
DLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNE
SPLKYLFRPEEIELLICGSRNLGFQALEETTEYDGGYTRDSVLIREFWEIVHSFTDEQ
KRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLK
ERLLKAITYAKGFGML"
BASE COUNT 1032 a 679 c 791 g 829 t
ORIGIN
1 ccaagatggt ggcgctgggc tcggggtgac tacaggagac gacggggcct tttcccttcg
61 ccaggacccg acacaccagg cttcgctcgc tcgcgcaccc ctccgccgcg tagccatccg
121 ccagcgcggg cgcccgccat ccgccgccta cttacgcttc acctctgccg acccggcgcg
181 ctcggctgcg ggcggcggcg cctccttcgg ctcctcctcg gaatagctcg cggcctgtag
241 cccctggcag gagggcccct cagccccccg gtgtggacag gcagcggcgg ctggcgacga
301 acgccgggat ttcggcggcc ccggcgctcc ctttcccggc ctcgttttcc ggataaggaa
361 gcgcgggtcc cgcatgagcc ccggcggtgg cggcagcgaa agagaacgag gcggtggcgg
421 gcggaggcgg cgggcgaggg cgactacgac cagtgaggcg gccgccgcag cccaggcgcg
481 ggggcgacga caggttaaaa atctgtaaga gcctgatttt agaattcacc agctcctcag
541 aagtttggcg aaatatgagt tattaagcct acgctcagat caaggtagca gctagactgg
601 tgtgacaacc tgtttttaat cagtgactca aagctgtgat caccctgatg tcaccgaatg
661 gccacagctt gtaaaagatc aggagaacct cagtctgacg acattgaagc tagccgaatg
721 aagcgagcag ctgcaaagca tctaatagaa cgctactacc accagttaac tgagggctgt
781 ggaaatgaag cctgcacgaa tgagttttgt gcttcctgtc caacttttct tcgtatggat
841 aataatgcag cagctattaa agccctcgag ctttataaga ttaatgcaaa actctgtgat
901 cctcatccct ccaagaaagg agcaagctca gcttaccttg agaactcgaa aggtgccccc
961 aacaactcct gctctgagat aaaaatgaac aagaaaggcg ctagaattga ttttaaagat
1021 gtgacttact taacagaaga gaaggtatat gaaattcttg aattatgtag agaaagagag
1081 gattattccc ctttaatccg tgttattgga agagtttttt ctagtgctga ggcattggta
1141 cagagcttcc ggaaagttaa acaacacacc aaggaagaac tgaaatctct tcaagcaaaa
1201 gatgaagaca aagatgaaga tgaaaaggaa aaagctgcat gttctgctgc tgctatggaa
1261 gaagactcag aagcatcttc ctcaaggata ggtgatagct cacagggaga caacaatttg
1321 caaaaattag gccctgatga tgtgtctgtg gatattgatg ccattagaag ggtctacacc
1381 agattgctct ctaatgaaaa aattgaaact gcctttctca atgcacttgt atatttgtca
1441 cctaacgtgg aatgtgactt gacgtatcac aatgtatact ctcgagatcc taattatctg
1501 aatttgttca ttatcgtaat ggagaataga aatctccaca gtcctgaata tctggaaatg
1561 gctttgccat tattttgcaa agcgatgagc aagctacccc ttgcagccca aggaaaactg
1621 atcagactgt ggtctaaata caatgcagac cagattcgga gaatgatgga gacatttcag
1681 caacttatta cttataaagt cataagcaat gaatttaaca gtcgaaatct agtgaatgat
1741 gatgatgcca ttgttgctgc ttcgaagtgc ttgaaaatgg tttactatgc aaatgtagtg
1801 ggaggggaag tggacacaaa tcacaatgaa gaagatgatg aagagcccat ccctgagtcc
1861 agcgagctga cacttcagga acttttggga gaagaaagaa gaaacaagaa aggtcctcga
1921 gtggaccccc tggaaactga acttggtgtt aaaaccctgg attgtcgaaa accacttatc
1981 ccttttgaag agtttattaa tgaaccactg aatgaggttc tagaaatgga taaagattat
2041 acttttttca aagtagaaac agagaacaaa ttctctttta tgacatgtcc ctttatattg
2101 aatgctgtca caaagaattt gggattatat tatgacaata gaattcgcat gtacagtgaa
2161 cgaagaatca ctgttctcta cagcttagtt caaggacagc agttgaatcc atatttgaga
2221 ctcaaagtta gacgtgacca tatcatagat gatgcacttg tccggctaga gatgatcgct
2281 atggaaaatc ctgcagactt gaagaagcag ttgtatgtgg aatttgaagg agaacaagga
2341 gttgatgagg gaggtgtttc caaagaattt tttcagctgg ttgtggagga aatcttcaat
2401 ccagatattg gtatgttcac atacgatgaa tctacaaaat tgttttggtt taatccatct
2461 tcttttgaaa ctgagggtca gtttactctg attggcatag tactgggtct ggctatttac
2521 aataactgta tactggatgt acattttccc atggttgtct acaggaagct aatggggaaa
2581 aaaggaactt ttcgtgactt gggagactct cacccagttc tatatcagag tttaaaagat
2641 ttattggagt atgaagggaa tgtggaagat gacatgatga tcactttcca gatatcacag
2701 acagatcttt ttggtaaccc aatgatgtat gatctaaagg aaaatggtga taaaattcca
2761 attacaaatg aaaacaggaa ggaatttgtc aatctttatt ctgactacat tctcaataaa
2821 tcagtagaaa aacagttcaa ggcttttcgg agaggttttc atatggtgac caatgaatct
2881 cccttaaagt acttattcag accagaagaa attgaattgc ttatatgtgg aagccggaat
2941 ctaggtttcc aagcactaga agaaactaca gaatatgacg gtggctatac cagggactct
3001 gttctgatta gggagttctg ggaaatcgtt cattcattta cagatgaaca gaaaagactc
3061 ttcttgcagt ttacaacggg cacagacaga gcacctgtgg gaggactagg aaaattaaag
3121 atgattatag ccaaaaatgg cccagacaca gaaaggttac ctacatctca tacttgcttt
3181 aatgtgcttt tacttccgga atactcaagc aaagaaaaac ttaaagagag attgttgaag
3241 gccatcacgt atgccaaagg atttggcatg ctgtaaaaca aaacaaaaca aaataaaaca
3301 aaaaaaagga aggaaaaaaa aaaaaaaaaa a
//