LOCUS BC002519 1627 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens polynucleotide kinase 3'-phosphatase, mRNA (cDNA clone
MGC:2582 IMAGE:3140712), complete cds.
ACCESSION BC002519
VERSION BC002519.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1627)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1627)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC002519.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 4 Row: c Column: 24.
FEATURES Location/Qualifiers
source 1..1627
/db_xref="H-InvDB:HIT000050409"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2582 IMAGE:3140712"
/tissue_type="Ovary, adenocarcinoma"
/clone_lib="NIH_MGC_9"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1627
/gene="PNKP"
/gene_synonym="PNK"
/db_xref="GeneID:11284"
/db_xref="MIM:605610"
CDS 76..1524
/gene="PNKP"
/gene_synonym="PNK"
/codon_start=1
/product="PNKP protein"
/protein_id="AAH02519.2"
/db_xref="GeneID:11284"
/db_xref="MIM:605610"
/translation="MQILTPPLQSSVELVADPETRTVAVKQLGVNPSTTGTQELKPGL
EGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMR
KSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEI
PRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGL
YRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFAL
NLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGF
PGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASR
ARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAP
TLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG"
misc_feature 466..831
/gene="PNKP"
/gene_synonym="PNK"
/note="HisB; Region: Histidinol phosphatase and related
phosphatases [Amino acid transport and metabolism]"
/db_xref="CDD:COG0241"
misc_feature 1051..1374
/gene="PNKP"
/gene_synonym="PNK"
/note="COG4639; Region: COG4639, Predicted kinase [General
function prediction only]"
/db_xref="CDD:COG4639"
BASE COUNT 379 a 494 c 480 g 274 t
ORIGIN
1 ggaccggaag tgctccagaa ctcaagcctg aagcaggcca cactccccag aaatgaggga
61 cccaatcaca caaggatgca aatcctgact ccacccctcc aatcctcagt ggagctggtc
121 gcagatcctg agacccggac agtggcagtg aaacagctgg gagttaaccc ctcaactacc
181 gggacccagg agttgaagcc ggggttggag ggctctctgg gggtggggga cacactgtat
241 ttggtcaatg gcctccaccc actgaccctg cgctgggaag agacccgcac accagaatcc
301 cagccagata ctccgcctgg cacccctctg gtgtcccaag atgagaagag agatgctgag
361 ctgccgaaga agcgtatgcg gaagtcaaac cccggctggg agaacttgga gaagttgcta
421 gtgttcaccg cagctggggt gaaaccccag ggcaaggtgg ctggctttga tctggacggg
481 acgctcatca ccacacgctc tgggaaggtc tttcccactg gccccagtga ctggaggatc
541 ttgtacccag agattccccg taagctccga gagctggaag ccgagggcta caagctggtg
601 atcttcacca accagatgag catcgggcgc gggaagctgc cagccgagga gttcaaggcc
661 aaggtggagg ctgtggtgga gaagctgggg gtccccttcc aggtgctggt ggccacgcac
721 gcaggcttgt accggaagcc ggtgacgggc atgtgggacc atctgcagga gcaggccaac
781 gacggcacgc ccatatccat cggggacagc atctttgtgg gagacgcagc cggacgcccg
841 gccaactggg ccccggggcg gaagaagaaa gacttctcct gcgccgatcg cctgtttgcc
901 ctcaaccttg gcctgccctt cgccacgcct gaggagttct ttctcaagtg gccagcagcc
961 ggcttcgagc tcccagcctt tgatccgagg actgtctccc gctcagggcc tctctgcctc
1021 cccgagtcca gggccctcct gagcgccagc ccggaggtgg ttgtcgcagt gggattccct
1081 ggggccggga agtccacctt tctcaagaag cacctcgtgt cggccggata tgtccacgtg
1141 aacagggaca cgctaggctc ctggcagcgc tgtgtgacca cgtgtgagac agccctgaag
1201 caagggaaac gggtcgccat cgacaacaca aacccagacg ccgcgagccg cgccaggtac
1261 gtccagtgtg cccgagccgc gggcgtcccc tgccgctgct tcctcttcac cgccactctg
1321 gagcaggcgc gccacaacaa ccggtttcga gagatgacgg actcctctca tatccccgtg
1381 tcagacatgg tcatgtatgg ctacaggaag cagttcgagg ccccaacgct ggctgaaggc
1441 ttctctgcca tcctggagat cccgttccgg ctatgggtgg agccgaggct ggggcggctg
1501 tactgccagt tctccgaggg ctgagccccg cccagctccc ctccacaata aacgctgttt
1561 ctccttgaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1621 aaaaaaa
//