LOCUS BC002480 1294 bp mRNA linear HUM 23-NOV-2007
DEFINITION Homo sapiens steroid 5 alpha-reductase 3, mRNA (cDNA clone MGC:3099
IMAGE:3350037), complete cds.
ACCESSION BC002480
VERSION BC002480.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1294)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1294)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC002480.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: l Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 13375784.
FEATURES Location/Qualifiers
source 1..1294
/db_xref="H-InvDB:HIT000030927"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3099 IMAGE:3350037"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_16"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1294
/gene="SRD5A3"
/gene_synonym="SRD5A2L"
/db_xref="GeneID:79644"
/db_xref="HGNC:HGNC:25812"
CDS 93..1049
/gene="SRD5A3"
/gene_synonym="SRD5A2L"
/codon_start=1
/product="steroid 5 alpha-reductase 3"
/protein_id="AAH02480.1"
/db_xref="GeneID:79644"
/db_xref="HGNC:HGNC:25812"
/translation="MAPWAEAEHSALNPLRAVWLTLTAAFLLTLLLQLLPPGLLPGCA
IFQDLIRYGKTKCGEPSRPAACRAFDVPKRYFSHFYIISVLWNGFLLWCLTQSLFLGA
PFPSWLHGLLRILGAAQFQGGELALSAFLVLVFLWLHSLRRLFECLYVSVFSNVMIHV
VQYCFGLVYYVLVGLTVLSQVPMDGRNAYITGKNLLMQARWFHILGMMMFIWSSAHQY
KCHVILGNLRKNKAGVVIHCNHRIPFGDWFEYVSSPNYLAELMIYVSMAVTFGFHNLT
WWLVVTNVFFNQALSAFLSHQFYKSKFVSYPKHRKAFLPFLF"
BASE COUNT 306 a 334 c 315 g 339 t
ORIGIN
1 ctgagaccgg tgcgccgcgc gctagtggcc gctcttccgc gggctagcgg gcggtggggg
61 cgccagcagc gcggaaggcg ggcacgcggg ccatggctcc ctgggcggag gccgagcact
121 cggcgctgaa cccgctgcgc gcggtgtggc tcacgctgac cgccgccttc ctgctgaccc
181 tactgctgca gctcctgccg cccggcctgc tcccgggctg cgcgatcttc caggacctga
241 tccgctatgg gaaaaccaag tgtggggagc cgtcgcgccc cgccgcctgc cgagcctttg
301 atgtccccaa gagatatttt tcccactttt atatcatctc agtgctgtgg aatggcttcc
361 tgctttggtg ccttactcaa tctctgttcc tgggagcacc ttttccaagc tggcttcatg
421 gtttgctcag aattctcggg gcggcacagt tccagggagg ggagctggca ctgtctgcat
481 tcttagtgct agtatttctg tggctgcaca gcttacgaag actcttcgag tgcctctacg
541 tcagtgtctt ctccaatgtc atgattcacg tcgtgcagta ctgttttgga cttgtctatt
601 atgtccttgt tggcctaact gtgctgagcc aagtgccaat ggatggcagg aatgcctaca
661 taacagggaa aaatctattg atgcaagcac ggtggttcca tattcttggg atgatgatgt
721 tcatctggtc atctgcccat cagtataagt gccatgttat tctcggcaat ctcaggaaaa
781 ataaagcagg agtggtcatt cactgtaacc acaggatccc atttggagac tggtttgaat
841 atgtttcttc ccctaactac ttagcagagc tgatgatcta cgtttccatg gccgtcacct
901 ttgggttcca caacttaact tggtggctag tggtgacaaa tgtcttcttt aatcaggccc
961 tgtctgcctt tctcagccac caattctaca aaagcaaatt tgtctcttac ccgaagcata
1021 ggaaagcttt cctaccattt ttgttttaag ttaacctcag tcatgaagaa tgcaaaccag
1081 gtgatggttt caatgcctaa ggacagtgaa gtctggagcc caaagtacag tttcagcaaa
1141 gctgtttgaa actctccatt ccatttctat accccacaag ttttcactga atgagcatgg
1201 cagtgccact caagaaaatg aatctccaaa gtatcttcaa agaataaata ctaatggcag
1261 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//