LOCUS BC002466 2442 bp mRNA linear HUM 25-JAN-2007
DEFINITION Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog, mRNA
(cDNA clone MGC:2356 IMAGE:3347509), complete cds.
ACCESSION BC002466
VERSION BC002466.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2442)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2442)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC002466.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: b Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4502192.
FEATURES Location/Qualifiers
source 1..2442
/db_xref="H-InvDB:HIT000030915"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2356 IMAGE:3347509"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2442
/gene="ARAF"
/gene_synonym="A-RAF"
/gene_synonym="PKS2"
/gene_synonym="RAFA1"
/db_xref="GeneID:369"
/db_xref="HGNC:HGNC:646"
/db_xref="MIM:311010"
CDS 92..1912
/gene="ARAF"
/gene_synonym="A-RAF"
/gene_synonym="PKS2"
/gene_synonym="RAFA1"
/codon_start=1
/product="v-raf murine sarcoma 3611 viral oncogene
homolog"
/protein_id="AAH02466.1"
/db_xref="GeneID:369"
/db_xref="HGNC:HGNC:646"
/db_xref="MIM:311010"
/translation="MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDS
LDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFV
RKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQD
LSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNV
HMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPA
EQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVA
VKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYH
HLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGL
ATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLP
YSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILAT
IELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP"
BASE COUNT 539 a 764 c 635 g 504 t
ORIGIN
1 tggagacggc ggcggctgta gcggcgtgac aggagcccca tggcacctgc ccagccccac
61 ctcagcccat cttgacaaaa tctaaggctc catggagcca ccacggggcc cccctgccaa
121 tggggccgag ccatcccggg cagtgggcac cgtcaaagta tacctgccca acaagcaacg
181 cacggtggtg actgtccggg atggcatgag tgtctacgac tctctagaca aggccctgaa
241 ggtgcggggt ctaaatcagg actgctgtgt ggtctaccga ctcatcaagg gacgaaagac
301 ggtcactgcc tgggacacag ccattgctcc cctggatggc gaggagctca ttgtcgaggt
361 ccttgaagat gtcccgctga ccatgcacaa ttttgtacgg aagaccttct tcagcctggc
421 gttctgtgac ttctgcctta agtttctgtt ccatggcttc cgttgccaaa cctgtggcta
481 caagttccac cagcattgtt cctccaaggt ccccacagtc tgtgttgaca tgagtaccaa
541 ccgccaacag ttctaccaca gtgtccagga tttgtccgga ggctccagac agcatgaggc
601 tccctcgaac cgccccctga atgagttgct aaccccccag ggtcccagcc cccgcaccca
661 gcactgtgac ccggagcact tccccttccc tgccccagcc aatgcccccc tacagcgcat
721 ccgctccacg tccactccca acgtccatat ggtcagcacc acggccccca tggactccaa
781 cctcatccag ctcactggcc agagtttcag cactgatgct gccggtagta gaggaggtag
841 tgatggaacc ccccggggga gccccagccc agccagcgtg tcctcgggga ggaagtcccc
901 acattccaag tcaccagcag agcagcgcga gcggaagtcc ttggccgatg acaagaagaa
961 agtgaagaac ctggggtacc gggactcagg ctattactgg gaggtaccac ccagtgaggt
1021 gcagctgctg aagaggatcg ggacgggctc gtttggcacc gtgtttcgag ggcggtggca
1081 tggcgatgtg gccgtgaagg tgctcaaggt gtcccagccc acagctgagc aggcccaggc
1141 tttcaagaat gagatgcagg tgctcaggaa gacgcgacat gtcaacatct tgctgtttat
1201 gggcttcatg acccggccgg gatttgccat catcacacag tggtgtgagg gctccagcct
1261 ctaccatcac ctgcatgtgg ccgacacacg cttcgacatg gtccagctca tcgacgtggc
1321 ccggcagact gcccagggca tggactacct ccatgccaag aacatcatcc accgagatct
1381 caagtctaac aacatcttcc tacatgaggg gctcacggtg aagatcggtg actttggctt
1441 ggccacagtg aagactcgat ggagcggggc ccagcccttg gagcagccct caggatctgt
1501 gctgtggatg gcagctgagg tgatccgtat gcaggacccg aacccctaca gcttccagtc
1561 agacgtctat gcctacgggg ttgtgctcta cgagcttatg actggctcac tgccttacag
1621 ccacattggc tgccgtgacc agattatctt tatggtgggc cgtggctatc tgtccccgga
1681 cctcagcaaa atctccagca actgccccaa ggccatgcgg cgcctgctgt ctgactgcct
1741 caagttccag cgggaggagc ggcccctctt cccccagatc ctggccacaa ttgagctgct
1801 gcaacggtca ctccccaaga ttgagcggag tgcctcggaa ccctccttgc accgcaccca
1861 ggccgatgag ttgcctgcct gcctactcag cgcagcccgc cttgtgcctt aggccccgcc
1921 caagccacca gggagccaat ctcagccctc cacgccaagg agccttgccc accagccaat
1981 caatgttcgt ctctgccctg atgctgcctc aggatccccc attccccacc ctgggagatg
2041 agggggtccc catgtgcttt tccagttctt ctggaattgg gggacccccg ccaaagactg
2101 agccccctgt ctcctccatc atttggtttc ctcttggctt tggggatact tctaaatttt
2161 gggagctcct ccatctccaa tggctgggat ttgtggcagg gattccactc agaacctctc
2221 tggaatttgt gcctgatgtg ccttccactg gattttgggg ttcccagcac cccatgtgga
2281 ttttgggggg tcccttttgt gtctcccccg ccattcaagg actcctctct ttcttcacca
2341 agaagcacag aattctgctg ggcctttgct tgtttaaaaa aaaaaaaaaa aaaaaaaaaa
2401 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
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