LOCUS BC002453 2379 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens heat shock 70kDa protein 1A, mRNA (cDNA clone MGC:1309
IMAGE:3345864), complete cds.
ACCESSION BC002453
VERSION BC002453.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2379)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2379)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC002453.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: d Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 26787973.
FEATURES Location/Qualifiers
source 1..2379
/db_xref="H-InvDB:HIT000030904_04"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1309 IMAGE:3345864"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2379
/gene="HSPA1A"
/gene_synonym="HSP70-1"
/gene_synonym="HSP72"
/gene_synonym="HSPA1B"
/db_xref="GeneID:3303"
/db_xref="HGNC:HGNC:5232"
/db_xref="MIM:140550"
CDS 178..2103
/gene="HSPA1A"
/gene_synonym="HSP70-1"
/gene_synonym="HSP72"
/gene_synonym="HSPA1B"
/codon_start=1
/product="heat shock 70kDa protein 1A"
/protein_id="AAH02453.1"
/db_xref="GeneID:3303"
/db_xref="HGNC:HGNC:5232"
/db_xref="MIM:140550"
/translation="MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF
TDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPK
VQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKD
AGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFE
VKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSS
STQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDL
VLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLL
LLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMT
KDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKG
RLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEAD
KKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGA
QGPKGGSGSGPTIEEVD"
BASE COUNT 546 a 676 c 738 g 419 t
ORIGIN
1 cctttttcga gagtgactcc cgttgtccca aggcttccca gagcgaacct gtgcggctgc
61 aggcaccggc gcgtcgagtt tccggcgtcc ggaaggaccg agctcttctc gcggatccag
121 tgttccgttt ccagccccca atctcagagc ggagccgaca gagagcaggg aaccggcatg
181 gccaaagccg cggcgatcgg catcgacctg ggcaccacct actcctgcgt gggggtgttc
241 caacacggca aggtggagat catcgccaac gaccagggca accgcaccac ccccagctac
301 gtggccttca cggacaccga gcggctcatc ggggatgcgg ccaagaacca ggtggcgctg
361 aacccgcaga acaccgtgtt tgacgcgaag cggctgatcg gccgcaagtt cggcgacccg
421 gtggtgcagt cggacatgaa gcactggcct ttccaggtga tcaacgacgg agacaagccc
481 aaggtgcagg tgagctacaa gggggagacc aaggcattct accccgagga gatctcgtcc
541 atggtgctga ccaagatgaa ggagatcgcc gaggcgtacc tgggctaccc ggtgaccaac
601 gcggtgatca ccgtgccggc ctacttcaac gactcgcagc gccaggccac caaggatgcg
661 ggtgtgatcg cggggctcaa cgtgctgcgg atcatcaacg agcccacggc cgccgccatc
721 gcctacggcc tggacagaac gggcaagggg gagcgcaacg tgctcatctt tgacctgggc
781 gggggcacct tcgacgtgtc catcctgacg atcgacgacg gcatcttcga ggtgaaggcc
841 acggccgggg acacccacct gggtggggag gactttgaca acaggctggt gaaccacttc
901 gtggaggagt tcaagagaaa acacaagaag gacatcagcc agaacaagcg agccgtgagg
961 cggctgcgca ccgcctgcga gagggccaag aggaccctgt cgtccagcac ccaggccagc
1021 ctggagatcg actccctgtt tgagggcatc gacttctaca cgtccatcac cagggcgagg
1081 ttcgaggagc tgtgctccga cctgttccga agcaccctgg agcccgtgga gaaggctctg
1141 cgcgacgcca agctggacaa ggcccagatt cacgacctgg tcctggtcgg gggctccacc
1201 cgcatcccca aggtgcagaa gctgctgcag gacttcttca acgggcgcga cctgaacaag
1261 agcatcaacc ccgacgaggc tgtggcctac ggggcggcgg tgcaggcggc catcctgatg
1321 ggggacaagt ccgagaacgt gcaggacctg ctgctgctgg acgtggctcc cctgtcgctg
1381 gggctggaga cggccggagg cgtgatgact gccctgatca agcgcaactc caccatcccc
1441 accaagcaga cgcagatctt caccacctac tccgacaacc aacccggggt gctgatccag
1501 gtgtacgagg gcgagagggc catgacgaaa gacaacaatc tgttggggcg cttcgagctg
1561 agcggcatcc ctccggcccc caggggcgtg ccccagatcg aggtgacctt cgacatcgat
1621 gccaacggca tcctgaacgt cacggccacg gacaagagca ccggcaaggc caacaagatc
1681 accatcacca acgacaaggg ccgcctgagc aaggaggaga tcgagcgcat ggtgcaggag
1741 gcggagaagt acaaagcgga ggacgaggtg cagcgcgaga gggtgtcagc caagaacgcc
1801 ctggagtcct acgccttcaa catgaagagc gccgtggagg atgaggggct caagggcaag
1861 atcagcgagg cggacaagaa gaaggttctg gacaagtgtc aagaggtcat ctcgtggctg
1921 gacgccaaca ccttggccga gaaggacgag tttgagcaca agaggaagga gctggagcag
1981 gtgtgtaacc ccatcatcag cggactgtac cagggtgccg gtggtcccgg gcctgggggc
2041 ttcggggctc agggtcccaa gggagggtct gggtcaggcc ccaccattga ggaggtggat
2101 taggggcctt tccaagattg ctgtttttgt tttggagctt caagactttg catttcctag
2161 tatttctgtt tgtcagttct caatttcctg tgtttgcaat gttgaaattt tttggtgaag
2221 tactgaactt gctttttttc cggtttctac atgcagagat gaatttatac tgccatctta
2281 cgactatttc ttctttttaa tacacttaac tcaggccatt ttttaagttg gttacttcaa
2341 agtaaataaa ctttaaaatt caaaaaaaaa aaaaaaaaa
//