LOCUS BC002406 1456 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1, mRNA (cDNA
clone MGC:8609 IMAGE:2961286), complete cds.
ACCESSION BC002406
VERSION BC002406.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1456)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1456)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: l Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505684.
FEATURES Location/Qualifiers
source 1..1456
/db_xref="H-InvDB:HIT000030868"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8609 IMAGE:2961286"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1456
/gene="PDHA1"
/gene_synonym="PDHCE1A"
/gene_synonym="PHE1A"
/db_xref="GeneID:5160"
/db_xref="HGNC:HGNC:8806"
/db_xref="MIM:300502"
CDS 58..1230
/gene="PDHA1"
/gene_synonym="PDHCE1A"
/gene_synonym="PHE1A"
/codon_start=1
/product="pyruvate dehydrogenase (lipoamide) alpha 1"
/protein_id="AAH02406.1"
/db_xref="GeneID:5160"
/db_xref="HGNC:HGNC:8806"
/db_xref="MIM:300502"
/translation="MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLH
RLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCV
GLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNF
YGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPC
IFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSG
KGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEE
LKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS"
BASE COUNT 408 a 302 c 406 g 340 t
ORIGIN
1 ccgcgtcgtg cctcctgggt tgtgaggagt cgccgctgcc gccactgcct gtgcttcatg
61 aggaagatgc tcgccgccgt ctcccgcgtg ctgtctggcg cttctcagaa gccggcaagc
121 agagtgctgg tagcatcccg taattttgca aatgatgcta catttgaaat taagaaatgt
181 gaccttcacc ggctggaaga aggccctcct gtcacaacag tgctcaccag ggaggatggg
241 ctcaaatact acaggatgat gcagactgta cgccgaatgg agttgaaagc agatcagctg
301 tataaacaga aaattattcg tggtttctgt cacttgtgtg atggtcagga agcttgctgt
361 gtgggcctgg aggccggcat caaccccaca gaccatctca tcacagccta ccgggctcac
421 ggctttactt tcacccgggg cctttccgtc cgagaaattc tcgcagagct tacaggacga
481 aaaggaggtt gtgctaaagg gaaaggagga tcgatgcaca tgtatgccaa gaacttctac
541 gggggcaatg gcatcgtggg agcgcaggtg cccctgggcg ctgggattgc tctagcctgt
601 aagtataatg gaaaagatga ggtctgcctg actttatatg gcgatggtgc tgctaaccag
661 ggccagatat tcgaagctta caacatggca gctttgtgga aattaccttg tattttcatc
721 tgtgagaata atcgctatgg aatgggaacg tctgttgaga gagcggcagc cagcactgat
781 tactacaaga gaggcgattt cattcctggg ctgagagtgg atggaatgga tatcctgtgc
841 gtccgagagg caacaaggtt tgctgctgcc tattgtagat ctgggaaggg gcccatcctg
901 atggagctgc agacttaccg ttaccacgga cacagtatga gtgaccctgg agtcagttac
961 cgtacacgag aagaaattca ggaagtaaga agtaagagtg accctattat gcttctcaag
1021 gacaggatgg tgaacagcaa tcttgccagt gtggaagaac taaaggaaat tgatgtggaa
1081 gtgaggaagg agattgagga tgctgcccag tttgccacgg ccgatcctga gccacctttg
1141 gaagagctgg gctaccacat ctactccagc gacccacctt ttgaagttcg tggtgccaat
1201 cagtggatca agtttaagtc agtcagttaa ggggaggaga aggagaggtt ataccttcag
1261 ggggctacca gacagtgttc tcaacttggt taaggaggaa gaaaacccag tcaatgaaat
1321 tcaatgaaat tcttggaaac ttccattaag tgtgtagatt gagcaggtag taattgcatg
1381 cagtttgtac attagtgcat taaaagatga attattgagt gaaaaaaaaa aaaaaaaaaa
1441 aaaaaaaaaa aaaaaa
//