LOCUS       BC001700                 539 bp    mRNA    linear   SYN 02-SEP-2016
DEFINITION  Synthetic construct Homo sapiens ribosomal protein L27, mRNA (cDNA
            clone MGC:2930 IMAGE:3050183), complete cds.
ACCESSION   BC001700
VERSION     BC001700.2
KEYWORDS    MGC.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2001) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     On Aug 20, 2003 this sequence version replaced BC001700.1.
            Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: ATCC/DCTD/DTP
            cDNA Library Preparation: Rubin Laboratory
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Institute for Systems Biology
            http://www.systemsbiology.org
            contact: amadan@systemsbiology.org
            Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
            Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAL Plate: 3 Row: p Column: 24
            This clone was selected for full length sequencing because it
            passed the following selection criteria: Hexamer frequency ORF
            analysis.
FEATURES             Location/Qualifiers
     source          1..539
                     /db_xref="H-InvDB:HIT000030586"
                     /organism="synthetic construct"
                     /mol_type="mRNA"
                     /isolation_source="Homo sapiens; Skin, melanotic
                     melanoma."
                     /db_xref="taxon:32630"
                     /clone="MGC:2930 IMAGE:3050183"
                     /clone_lib="NIH_MGC_20"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            1..539
                     /gene="RPL27"
                     /db_xref="GeneID:6155"
                     /db_xref="HGNC:HGNC:10328"
                     /db_xref="MIM:607526"
     CDS             69..479
                     /gene="RPL27"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L27"
                     /protein_id="AAH01700.1"
                     /db_xref="GeneID:6155"
                     /db_xref="HGNC:HGNC:10328"
                     /db_xref="MIM:607526"
                     /translation="MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVA
                     GIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFR
                     DPALKRKARREAKVKFEERYKTGKNKWFFQKLRF"
BASE COUNT          173 a          108 c          129 g          129 t
ORIGIN      
        1 gagagagaga actagtctcg agtttttttt tttttttttt gctgtaggcc cgggtggttg
       61 ctgccgaaat gggcaagttc atgaaacctg ggaaggtggt gcttgtcctg gctggacgct
      121 actccggacg caaagctgtc atcgtgaaga acattgatga tggcacctca gatcgcccct
      181 acagccatgc tctggtggct ggaattgacc gctacccccg caaagtgaca gctgccatgg
      241 gcaagaagaa gatcgccaag agatcaaaga taaaatcttt tgtgaaagtg tataactaca
      301 atcacctaat gcccacaagg tactctgtgg atatcccctt ggacaaaact gtcgtcaata
      361 aggatgtctt cagagatcct gctcttaaac gcaaggcccg acgggaggcc aaggtcaagt
      421 ttgaagagag atacaagaca ggcaagaaca agtggttctt ccagaaactg cggttttaga
      481 tgctttgttt tgatcattaa aaattataaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//