LOCUS BC001357 2205 bp mRNA linear HUM 11-SEP-2007
DEFINITION Homo sapiens LAG1 homolog, ceramide synthase 2, mRNA (cDNA clone
MGC:987 IMAGE:3051086), complete cds.
ACCESSION BC001357
VERSION BC001357.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2205)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2205)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-DEC-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC001357.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 4 Row: a Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32455257.
FEATURES Location/Qualifiers
source 1..2205
/db_xref="H-InvDB:HIT000030343"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:987 IMAGE:3051086"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2205
/gene="LASS2"
/gene_synonym="CerS2"
/gene_synonym="L3"
/gene_synonym="SP260"
/gene_synonym="TMSG1"
/db_xref="GeneID:29956"
/db_xref="HGNC:HGNC:14076"
/db_xref="MIM:606920"
CDS 82..1224
/gene="LASS2"
/gene_synonym="CerS2"
/gene_synonym="L3"
/gene_synonym="SP260"
/gene_synonym="TMSG1"
/codon_start=1
/product="LAG1 homolog, ceramide synthase 2"
/protein_id="AAH01357.2"
/db_xref="GeneID:29956"
/db_xref="HGNC:HGNC:14076"
/db_xref="MIM:606920"
/translation="MLQTLYDYFWWERLWLPVNLTWADLEDRDGRVYAKASDLYITLP
LALLFLIVRYFFELYVATPLAALLNIKEKTRLRAPPNATLEHFYLTSGKQPKQVEVEL
LSRQSGLSGRQVERWFRRRRNQDRPSLLKKFREASWRFTFYLIAFIAGMAVIVDKPWF
YDMKKVWEGYPIQSTIPSQYWYYMIELSFYWSLLFSIASDVKRKDFKEQIIHHVATII
LISFSWFANYIRAGTLIMALHDSSDYLLESAKMFNYAGWKNTCNNIFIVFAIVFIITR
LVILPFWILHCTLVYPLELYPAFFGYYFFNSMMGVLQLLHIFWAYLILRMAHKFITGK
LVEDERSDREETESSEGEEAAAGGGAKSRPLANGHPILNNNHRKND"
BASE COUNT 536 a 586 c 503 g 580 t
ORIGIN
1 gcctcggggc ctcggagcaa ccacccgagc agacggagta cacggagcag cggccccggc
61 cccgccaacg ctgccgccgg gatgctccag accttgtatg attacttctg gtgggaacgt
121 ctgtggctgc ctgtgaactt gacctgggcc gatctagaag accgagatgg acgtgtctac
181 gccaaagcct cagatctcta tatcacgctg cccctggcct tgctcttcct catcgttcga
241 tacttctttg agctgtacgt ggctacacca ctggctgccc tcttgaacat aaaggagaaa
301 actcggctgc gggcacctcc caacgccacc ttggaacatt tctacctgac cagtggcaag
361 cagcccaagc aggtggaagt agagcttttg tcccggcaga gcgggctctc tggccgccag
421 gtagagcgtt ggttccgtcg ccgccgcaac caggaccggc ccagtctcct caagaagttc
481 cgagaagcca gctggagatt cacattttac ctgattgcct tcattgccgg catggccgtc
541 attgtggata aaccctggtt ctatgacatg aagaaagttt gggagggata tcccatacag
601 agcactatcc cttcccagta ttggtactac atgattgaac tttccttcta ctggtccctg
661 ctcttcagca ttgcctctga tgtcaagcga aaggatttca aggaacagat catccaccat
721 gtggccacca tcattctcat cagcttttcc tggtttgcca attacatccg agctgggact
781 ctaatcatgg ctctgcatga ctcttccgat tacctgctgg agtcagccaa gatgtttaac
841 tacgcgggat ggaagaacac ctgcaacaac atcttcatcg tcttcgccat tgtttttatc
901 atcacccgac tggtcatcct gcccttctgg atcctgcatt gcaccctggt gtacccactg
961 gagctctatc ctgccttctt tggctattac ttcttcaatt ccatgatggg agttctacag
1021 ctgctgcata tcttctgggc ctacctcatt ttgcgcatgg cccacaagtt cataactgga
1081 aagctggtag aagatgaacg cagtgaccgg gaagaaacag agagctcaga gggggaggag
1141 gctgcagctg ggggaggagc aaagagccgg cccctagcca atggccaccc catcctcaat
1201 aacaaccatc gtaagaatga ctgaaccatt attccagctg cctcccagat taatgcataa
1261 agccaaggaa ctaccctgct ccctgcgcta tagggtcact ttaagctctg gggaaaaagg
1321 agaaagtgag aggagagttc tctgcatcct ccctccttgc ttgtcaccca gttgccttta
1381 aaccaaattc taaccagcct atccccaggt agggggacgt tggttatatt ctgttagagg
1441 gggacggtcg tattttcctc cctacccgcc aagtcatcct ttctactgct tttgaggccc
1501 tccctcagct ctctgtgggt aggggttaca attcacattc cttattctga gaatttggcc
1561 ccagctgttt gcctttgact ccctgacctc cagagccagg gttgtgcctt attgtcccat
1621 ctgtgggcct cattctgcca aagctggacc aaggctaacc tttctaagct ccctaacttg
1681 ggccagaaac caaagctgag cttttaactt tctccctcta tgacacaaat gaattgaggg
1741 taggaggagg gtgcacataa cccttaccct acctctgcca aaaagtgggg gctgtactgg
1801 ggactgctcg gatgatcttt cttagtgcta cttctttcag ctgtccctgt agcgacaggt
1861 ctaagatctg actgcctcct ttctctggcc tcttccccct tccctcttct cttcagctag
1921 gctagctggt ttggagtaga atggcaacta attctaattt ttatttatta aatatttggg
1981 gttttggttt taaagccaga attacggcta gcacctagca tttcagcaga gggaccattt
2041 tagaccaaaa tgtactgtta atgggttttt tttttaaaat taaaagatta aataaaaaat
2101 attaaataaa acatggcaat aagtgtcaga ctattaggaa ttgagaaggg ggatcaacta
2161 aataaacgaa gagagtcttt cttaaaaaaa aaaaaaaaaa aaaaa
//