LOCUS BC001346 1353 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens cytochrome b5 reductase 2, mRNA (cDNA clone MGC:4869
IMAGE:3451742), complete cds.
ACCESSION BC001346
VERSION BC001346.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1353)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1353)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 4 Row: c Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47778926
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1353
/db_xref="H-InvDB:HIT000030332"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4869 IMAGE:3451742"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1353
/gene="CYB5R2"
/gene_synonym="B5R.2"
/db_xref="GeneID:51700"
/db_xref="HGNC:HGNC:24376"
/db_xref="MIM:608342"
CDS 121..834
/gene="CYB5R2"
/gene_synonym="B5R.2"
/codon_start=1
/product="CYB5R2 protein"
/protein_id="AAH01346.1"
/db_xref="GeneID:51700"
/db_xref="HGNC:HGNC:24376"
/db_xref="MIM:608342"
/translation="MNSRRREPITLQDPEAKYPLPLIEKEKISHNTRRFRFGLPSPDH
VLGLPVGNYVQLLAKIDNELVVRAYTPVSSDDDRGFVDLIIKIYFKNVHPQYPEGGKM
TQYLENMKIGETIFFRGPRGRLFYHGPGNLGIRPDQTSEPKKTLADHLGMIAGGTGIT
PMLQLIRHITKDPSDRTRMSLIFANQTEEDILVRKELEEIARTHPDQFDLWYTLDRPP
IGPWSAEGATLLSNSAQFH"
BASE COUNT 344 a 368 c 336 g 305 t
ORIGIN
1 ggcaactttc cctgtcgggc ttgagttggg agaggagcag ggcggccttg tagggacccg
61 tccctgctcc tgaccatcac cgtcactggg gtcactgtgc tcgtgttggt cctgaagagc
121 atgaactcca ggaggagaga gccaatcacc ttacaggacc ctgaagccaa gtacccgctg
181 cccttgattg agaaagagaa aatcagccac aacacccgga ggttccgctt tggactgcct
241 tcgccggacc atgtcttagg gcttcctgta ggtaactatg tccagctctt ggcaaaaatc
301 gataatgaat tggtggtcag ggcttacacc cctgtctcca gtgatgatga cagaggcttt
361 gtggacctaa ttataaagat ctacttcaaa aatgtacacc cccaatatcc tgaaggtggg
421 aagatgactc agtatttgga gaacatgaaa atcggggaga ccatcttttt tcgagggcca
481 aggggacgct tgttttacca tgggccaggg aatcttggaa tcagaccaga ccagacgagt
541 gagcctaaaa aaacactggc cgatcacctg ggaatgattg ctgggggcac aggcatcaca
601 cccatgttgc agctcattcg ccacatcacc aaggacccca gtgacaggac caggatgtcc
661 ctcatctttg ccaaccagac agaggaggat atcttggtca gaaaagagct tgaagaaatt
721 gccaggactc acccagacca gttcgacctg tggtacaccc tggacaggcc tcccattggt
781 ccctggtcgg cagagggcgc taccctactg agcaattctg cgcagttcca ctaacaccag
841 gccacaccct gccggacaac tccagctggc tgtactgatt cacacacctc ggctggaagt
901 acagctcagg cttcgttact gccgacatga tcaaggagca ccttcctcct ccagcgaagt
961 ccacgctcat cctggtgtgt ggcccgccac cactgatcca gacggcggct caccctaacc
1021 tggagaagct gggttatacc caggacatga ttttcaccta ctaacaaaca cctccatgtg
1081 ctcagcaaat ttgcatgtcc cttttcatct gtttcagagt aagttcaatt tcaccacggt
1141 aaactgggat gttttcaaaa gtgccttgcc atgtaccttc gcgcacacac tggttctcct
1201 cttttgggtg tgggcctaac aaaaagggct caaggggctg gagactggct gctggggcct
1261 ccttgcttgg aggctggcaa gagctccatt tcagtatctt tctccgtggt tttgtgaaat
1321 aaactcaagt acaaagcaaa aaaaaaaaaa aaa
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