LOCUS BC001133 1666 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens asparagine-linked glycosylation 8,
alpha-1,3-glucosyltransferase homolog (S. cerevisiae), mRNA (cDNA
clone MGC:2840 IMAGE:2966827), complete cds.
ACCESSION BC001133
VERSION BC001133.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1666)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1666)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Oct 28, 2003 this sequence version replaced BC001133.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 3 Row: e Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 55743089.
FEATURES Location/Qualifiers
source 1..1666
/db_xref="H-InvDB:HIT000030163"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2840 IMAGE:2966827"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1666
/gene="ALG8"
/db_xref="GeneID:79053"
/db_xref="HGNC:HGNC:23161"
/db_xref="MIM:608103"
CDS 49..1629
/gene="ALG8"
/codon_start=1
/product="asparagine-linked glycosylation 8,
alpha-1,3-glucosyltransferase homolog (S. cerevisiae)"
/protein_id="AAH01133.1"
/db_xref="GeneID:79053"
/db_xref="HGNC:HGNC:23161"
/db_xref="MIM:608103"
/translation="MAALTIATGTGNWFSALALGVTLLKCLLIPTYHSTDFEVHRNWL
AITHSLPISQWYYEATSEWTLDYPPFFAWFEYILSHVAKYFDQEMLNVHNLNYSSSRT
LLFQRFSVIFMDVLFVYAVRECCKCIDGKKVGKELTEKPKFILSVLLLWNFGLLIVDH
IHFQYNGFLFGLMLLSIARLFQKRHMEGAFLFAVLLHFKHIYLYVAPAYGVYLLRSYC
FTANKPDGSIRWKSFSFVRVISLGLVVFLVSALSLGPFLALNQLPQVFSRLFPFKRGL
CHAYWAPNFWALYNALDKVLSVIGLKLKFLDPNNIPKASMTSGLVQQFQHTVLPSVTP
LATLICTLIAILPSIFCLWFKPQGPRGFLRCLTLCALSSFMFGWHVHEKAILLAILPM
SLLSVGKAGDASIFLILTTTGHYSLFPLLFTAPELPIKILLMLLFTIYSISSLKTLFR
KEKPLFNWMETFYLLGLGPLEVCCEFVFPFTSWKVKYPFIPLLLTSVYCAVGVTYAWF
KLYVSVLIDSAIGKTKKQ"
BASE COUNT 407 a 377 c 330 g 552 t
ORIGIN
1 gatcccggat gtggatcaag ttggtgggaa gcgtgcggtg ccgcagcaat ggcggcgctc
61 acaattgcca cgggtactgg caattggttt tcggctttgg cgctcggggt gactcttctc
121 aaatgccttc tcatccccac ataccattcc acagattttg aagtacaccg aaactggctt
181 gctatcactc acagtttgcc aatatcacag tggtattatg aggcaacttc agagtggacg
241 ttggattacc cccctttctt tgcatggttt gagtatatcc tgtcacatgt tgccaaatat
301 tttgatcaag aaatgctgaa tgtccataat ttgaattact ccagctcaag gaccttactt
361 ttccagagat tttccgtcat ctttatggat gtactctttg tgtatgctgt ccgtgagtgc
421 tgtaaatgca ttgatggaaa aaaagtgggt aaagaactta cagaaaagcc aaaatttatt
481 ctgtcggtat tacttctgtg gaacttcggg ttattaattg tggaccatat tcattttcag
541 tacaatggct ttttatttgg attaatgcta ctctccattg cacgattatt tcagaaaagg
601 catatggaag gagcatttct ctttgctgtt ctcctacatt tcaagcatat ctacctctat
661 gtagcaccag cttatggtgt atatctgctg cgatcctact gtttcactgc aaataaacca
721 gatgggtcta ttcgatggaa gagtttcagc tttgttcgtg ttatttccct gggactggtt
781 gttttcttag tttctgctct ttcattgggt cctttcctgg ccttgaatca gctgcctcaa
841 gtcttttccc gactctttcc tttcaagagg ggcctctgtc atgcatattg ggctccaaac
901 ttctgggctt tgtacaatgc tttggacaaa gtgctgtctg tcatcggttt gaaattgaaa
961 tttcttgatc ccaacaatat tcccaaggcc tcaatgacaa gtggtttggt tcagcagttc
1021 caacacacag tccttccctc agtgactccc ttggcaaccc tcatctgcac actgattgcc
1081 atattgccct ctattttctg tctttggttt aaaccccaag ggcccagagg ctttctccga
1141 tgtctaactc tttgtgcctt gagctccttt atgtttgggt ggcatgttca tgaaaaagcc
1201 atacttctag caattctccc aatgagcctt ttgtctgtgg gaaaagcagg agacgcttcg
1261 atttttctga ttctgaccac aacaggacat tattccctct ttcctctgct cttcactgca
1321 ccagaacttc ccattaaaat cttactcatg ttactattca ccatatatag tatttcgtca
1381 ctgaagactt tattcagaaa agaaaaacct ctttttaatt ggatggaaac tttctacctg
1441 cttggcctgg ggcctctgga agtctgctgt gaatttgtat tccctttcac ctcctggaag
1501 gtgaagtacc ccttcatccc tttgttacta acctcagtgt attgtgcagt aggcgtcaca
1561 tatgcttggt tcaaactgta tgtttcagta ttgattgact ctgctattgg caagacaaag
1621 aaacaatgaa taaaggaact gcttagaaaa aaaaaaaaaa aaaaaa
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