LOCUS BC000973 2497 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens G2/M-phase specific E3 ubiquitin ligase, mRNA (cDNA
clone MGC:4967 IMAGE:3450488), complete cds.
ACCESSION BC000973
VERSION BC000973.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2497)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2497)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (16-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Feb 23, 2001 this sequence version replaced BC000973.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: h Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33620748.
FEATURES Location/Qualifiers
source 1..2497
/db_xref="H-InvDB:HIT000030037"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4967 IMAGE:3450488"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2497
/gene="G2E3"
/gene_synonym="PHF7B"
/db_xref="GeneID:55632"
/db_xref="HGNC:HGNC:20338"
/db_xref="MIM:611299"
CDS 101..2221
/gene="G2E3"
/gene_synonym="PHF7B"
/codon_start=1
/product="G2/M-phase specific E3 ubiquitin ligase"
/protein_id="AAH00973.2"
/db_xref="GeneID:55632"
/db_xref="HGNC:HGNC:20338"
/db_xref="MIM:611299"
/translation="MNESKPGDSQNLACVFCRKHDDCPNKYGEKKTKEKWNLTVHYYC
LLMSSGIWQRGKEEEGVYGFLIEDIRKEVNRASKLKCCVCKKNGASIGCVAPRCKRSY
HFPCGLQRECIFQFTGNFASFCWDHRPVQIITSNNYRESLPCTICLEFIEPIPSYNIL
RSPCCKNAWFHRDCLQVQAINAGVFFFRCTICNNSDIFQKEMLRMGIHIPEKDASWEL
EENAYQELLQHYERCDVRRCRCKEGRDYNAPDSKWEIKRCQCCGSSGTHLACSSLRSW
EQNWECLECRGIIYNSGEFQKAKKHVLPNSNNVGITDCLLEESSPKLPRQSPGSQSKD
LLRQGSKFRRNVSTLLIELGFQIKKKTKRLYINKANIWNSALDAFRNRNFNPSYAIEV
AYVIENDNFGSEHPGSKQEFLSLLMQHLENSSLFEGSLSKNLSLNSQALKENLYYEAG
KMLAISLVHGGPSPGFFSKTLFNCLVYGPENTQPILDDVSDFDVAQIIIRINTATTVA
DLKSIINECYNYLELIGCLRLITTLSDKYMLVKDILGYHVIQRVHTPFESFKQGLKTL
GVLEKIQAYPEAFCSILCHKPESLSAKILSELFTVHTLPDVKALGFWNSYLQAVEDGK
STTTMEDILIFATGCSSIPPAGFKPTPSIECLHVDFPVGNKCNNCLAIPITNTYKEFQ
ENMDFTIRNTLRLEKEESSHYIGH"
BASE COUNT 821 a 426 c 491 g 759 t
ORIGIN
1 cgttgaatgt ggctgctcgc ggtcggcgtg ccccgacgta cagcgggccg ggaaaagtgg
61 cactgaggct ctggaacttc tgcccagctc tccttgtaaa atgaatgaaa gtaaacctgg
121 tgactcacag aaccttgctt gtgttttctg tcgaaaacat gatgactgtc ctaataaata
181 cggagaaaag aaaactaagg agaaatggaa tctcactgta cattactact gtttgttgat
241 gtcaagtgga atttggcaga gaggcaaaga agaagaagga gtttatggtt ttctaataga
301 agatatcagg aaggaagtga atagagcttc taaactgaaa tgctgtgttt gcaagaaaaa
361 tggtgcttca attggatgtg ttgcaccccg atgtaaacga agttatcatt tcccatgtgg
421 acttcagaga gaatgtattt tccagtttac tggcaatttt gcgtcatttt gttgggacca
481 tcgacctgtt caaataatta catctaataa ttatagagag tccttaccat gcaccatttg
541 cttggaattt attgagccta ttccaagtta taacatatta cgaagtcctt gttgtaagaa
601 cgcttggttt catagagact gtttacaggt tcaagcaata aatgcgggag tgtttttctt
661 taggtgtaca atatgcaata atagtgacat ctttcagaaa gagatgttga gaatgggaat
721 tcatattcct gaaaaagatg cttcctggga attagaggaa aacgcttatc aagagcttct
781 gcagcactat gagcgttgtg atgttcgaag atgtcgttgc aaagaagggc gagactataa
841 tgcacctgat agcaaatggg aaataaagcg ctgtcagtgt tgtggttcca gtggcacaca
901 tttagcctgc tcctcattac ggtcatggga gcaaaattgg gagtgtttgg aatgtagggg
961 tattatctac aattcaggag agttccaaaa agccaaaaaa catgtattac ccaattctaa
1021 taatgtgggg attacagatt gtttgttgga agagtcatca cctaaattac ccagacagtc
1081 acctggatcc cagagtaaag atctactgag gcaaggcagc aaatttagaa gaaatgtatc
1141 aacactatta atagagttag gattccaaat taaaaaaaaa actaaaagat tgtatatcaa
1201 caaagccaat atctggaata gtgccttaga tgcattcaga aatcgaaact ttaatccttc
1261 atatgcaatt gaagtagcat atgttattga aaatgataat tttggaagtg agcatcctgg
1321 atcaaagcaa gaatttctga gtctcttaat gcaacatctt gagaactcat cattgtttga
1381 agggtccttg tcaaagaact tgtctctaaa ttctcaagct ctgaaagaga atctttacta
1441 tgaagctggc aaaatgcttg ccatttcttt agttcacggt ggtccttcac ctggtttctt
1501 ttctaaaacc ttgtttaact gccttgttta tggaccagaa aatacccagc caattttaga
1561 tgatgtttca gactttgatg tggcacagat tataatcagg ataaatactg caacaactgt
1621 agctgactta aagtcaataa taaatgaatg ctataactac cttgagttaa ttggatgtct
1681 cagacttata acgacattaa gtgataaata tatgttagta aaagacatac ttggctacca
1741 tgtaattcag agagtccaca caccctttga aagttttaag cagggtctga aaacccttgg
1801 tgttttggag aaaattcagg cttatccaga agcattttgt agcatcctgt gtcataaacc
1861 tgagagtctt tctgcaaaaa tccttagtga gctttttaca gtacacacat tacctgatgt
1921 gaaagctttg gggttttgga acagttactt acaggctgtt gaagatggta aatctacaac
1981 aacaatggaa gacattctta tttttgcaac tggttgcagt tccattcctc cagctggatt
2041 taaacccact ccttcaattg agtgtctgca tgtggatttt cctgttggaa acaagtgtaa
2101 taactgttta gcaattccca tcaccaatac atataaagag tttcaagaaa atatggactt
2161 caccataaga aacactctaa gactagaaaa ggaagaaagt tctcattaca ttggacatta
2221 aaatgtttcc ttgaacaaag agaagcttct ttaaaagctg ctattgataa ctctctttta
2281 tttcactaac cttttcatca tcactttgaa caaactagtt agcttcttga cctaataaaa
2341 tttatgatat gaatataaag gaatattata gccacttagg ctaaaaaaag gttttttttg
2401 ttaaagcata ctagtcaaaa ttggtgataa tttctcattt tcttgatata gatacttcat
2461 aaacctcaat ataaatactc tcaaaaaaaa aaaaaaa
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